›› 2014, Vol. 26 ›› Issue (3): 0-730735.

• 植物保护 •    

转cry1C和cry2A基因Bt水稻对白背飞虱生长、发育及繁殖的继代影响

刘凯1,2,杨亚军2,*,田俊策2,徐红星2,郑许松2,吕仲贤1,2,*
  

  1. 1浙江师范大学 化学与生命科学学院,浙江 金华 321004;2浙江省农业科学院 植物保护与微生物研究所,浙江 杭州310021
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2014-05-25 发布日期:2014-05-25

Multigeneration effect of Bt rice with cry1C and cry2A on survival, development and reproduction of nontarget pest Sogatella furcifera (Horvth)

LIU Kai;YANG Yajun;*;TIAN Junce;XU Hongxing;ZHENG Xusong;LYU Zhongxian;*   

  1. 1 College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China; 2 Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2014-05-25 Published:2014-05-25

摘要: 转Bt基因水稻对非靶标节肢动物的影响是转基因水稻安全性评价的重要组成部分。目前,已开展了大量关于转Bt基因水稻对非靶标害虫安全性评价的研究,但多限于1代,不能完全反映田间情况。试验以转Bt基因水稻T1C19(含cry1C基因)和T2A1(含cry2A基因)以及其亲本非Bt水稻MH63为材料,在室内条件下连续饲养白背飞虱4代,研究转Bt水稻对非靶标害虫白背飞虱生长发育和繁殖的继代效应,以明确T1C19和T2A1水稻对白背飞虱的潜在影响。结果表明,白背飞虱在3种水稻品种上连续取食4代以后,其生长发育(若虫发育历期、若虫存活率、雌成虫体重)和繁殖(产卵量和卵孵化率)指标均没有受到水稻品种的显著影响。可见,供试的T1C19和T2A1对白背飞虱无显著影响。

关键词: 转Bt水稻, 白背飞虱, 生长发育, 繁殖, 安全评价

Abstract: The assessment on the effect of Bt rice on nontarget arthropods is one of the important items in the ecological safety assessment of Bt rice. So far, many studies on the effect of Bt rice on nontarget insect pests were conducted. However, most of experiments were focus on the assessment for only one generation of nontarget organisms, which could not completely simulate the field condition. In present study, the nontarget pest, white backed planthopper (WBPH), Sogatella furcifera(Horvth), was successively reared on Bt rice varieties, T1C19 with cry1C gene and T2A1 with cry2A gene, and their parental rice MH63 for four generations and the multigeneration effects of Bt rice on survival, development and reproduction of WBPH were evaluated to assess the potential impact of Bt rice on WBPH. The results indicated that the nymph duration and survival rate, female adult weight, longevity and fecundity, and the egg hatchability of WBPH on Bt rice did not significantly differ from those on MH63 over four generations. It implies that T1C19 and T2A1 had no significant effect on nontarget pest WBPH.

Key words: transgenic Bt rice, Sogatella furcifera, development, reproduction, safety assessment