浙江农业学报

• 植物保护 • 上一篇    下一篇

班氏跳小蜂对毒死蜱和吡虫啉的敏感性及解毒酶活性的变化

  

  1. (1 浙江农林大学 农业与食品科学学院,浙江 杭州311300;2 浙江省农业科学院 植物保护与微生物研究所,浙江省植物有害生物防控重点实验室—省部共建国家重点实验室培育基地,浙江 杭州310021)
  • 出版日期:2015-03-25 发布日期:2015-03-31

The sensitivity of Aenasius bambawalei to chlorpyrifos and imidacloprid and its detoxification enzyme activities

  1. (1 College of Agriculture and Food Science,Zhejiang Agriculture and Forestry University,Hangzhou 311300,China; 2 State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control,Institute of Plant Protection and Microbiology,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China)
  • Online:2015-03-25 Published:2015-03-31

摘要: 要:采用喷雾法和浸叶法测定毒死蜱、吡虫啉对班氏跳小蜂(Aenasius bambawalei Hayat)成蜂的毒力,并用喷雾法测定毒死蜱、吡虫啉对扶桑绵粉蚧体内班氏跳小蜂的发育、寄生率和相关解毒酶的活性。结果表明,毒死蜱喷药和浸叶处理的LC50分别为2595和0112 μg·L-1,吡虫啉喷药和浸叶处理的LC50分别为0115和0234 mg·L-1,毒死蜱对班氏跳小蜂的杀伤力较吡虫啉强;2种药剂处理下,班氏跳小蜂的雄、雌蜂的后足胫节长度与对照组差异不显著,对扶桑绵粉蚧的寄生率与对照组差异显著;毒死蜱对班氏跳小蜂体内乙酰胆碱酯酶的抑制率为764%,吡虫啉对班氏跳小蜂体内羧酸酯酶、多功能氧化酶的抑制率分别为853%和322%。

关键词: 班氏跳小蜂, 毒死蜱, 吡虫啉, 敏感性, 解毒酶活性

Abstract: In this paper,LC50 of chlorpyrifos and imidacloprid to Aenasius bambawalei were estimated by spray and leaf\|dipping methods,the individual development together with parasitization efficiency and some relative detoxification enzymes of the parasitoids were determined by spray method. The results showed that 1) by spray,LC50 to chlorpyrifos was 2595 μg·L-1,and imidacloprid was 0115 mg·L-1;2) by leaf dipping method,chlorpyrifos was 0112 μg·L-1,and imidacloprid was 0234 mg·L-1;and 3) the lethality of chlorpyrifos was higher than imidacloprid. These two pesticides had significant effects on the parasitization efficiency of the parasitoid,but no effect on the hind tibia length of the adults. Activity of AchE after chlorpyrifos treatment was inhibited by 764%,while activities of CarE and MFO in the imidacloprid treatments were inhibited by 853% and 322%.

Key words: Aenasius bambawalei, chlorpyrifos, imidacloprid, sensitivity, detoxification enzyme activity