浙江农业学报

• 园艺科学 • 上一篇    下一篇

砂糖椰子对干旱和水分补偿的生理变化

  

  1. (1. 厦门市园林植物园,福建 厦门 361003; 2. 国家林业局棕榈植物保育中心,福建 厦门 361003)
  • 出版日期:2016-01-25 发布日期:2016-01-19

Physiological changes of Arenga pinnata to drought stress and re\|watering process

  1. (1. Xiamen Botanical Garden, Xiamen 361003, China; 2. Palm Conservation & Breeding Center of National Forestry Bureau, Xiamen 361003, China)
  • Online:2016-01-25 Published:2016-01-19

摘要: 为了研究干旱胁迫和复水对砂糖椰子(Arenga pinnata)幼苗叶片和根系的影响,以两年生砂糖椰子幼苗为材料,分别进行干旱胁迫0,7,14和21 d,再复水3和6 d,然后测定叶片和根系的超氧化物歧化酶(SOD)活性、脯氨酸(Pro)含量、可溶性蛋白含量、丙二醛(MDA)含量、相对电导率以及叶绿素含量。结果表明,叶片的SOD活性在各个处理中均呈显著的波动性变化,根系的SOD活性在干旱胁迫和复水后都显著高于对照。叶片和根系的Pro含量在重度干旱胁迫下显著升高,复水后显著降低;而可溶性蛋白含量在中度和重度干旱胁迫时均显著减少,复水3 d后显著增加;叶片和根系的MDA含量变化趋势具有互补性;叶片和根系的相对电导率均随着干旱胁迫时间的延长而增加,在中度和重度干旱胁迫时显著高于其他处理,复水后减少;叶绿素含量在重度干旱胁迫时达到最高值。总之,在干旱胁迫下,叶片和根系不同指标变化规律不一样,脯氨酸含量、可溶性蛋白含量和相对电导率在叶片和根系中的变化趋势相似,SOD活性和MDA含量的变化趋势不一致,表明砂糖椰子地上部和地下部对干旱胁迫的响应具有协同效应。

关键词: 砂糖椰子, 干旱胁迫, 复水, 叶片, 根系

Abstract: To evaluate the physiological response of Arenga pinnata to drought stress and re\|watering process, activity of superoxide dismutase (SOD), the contents of proline, soluble protein and malondialdehyde (MDA), relative electrical conductivity and chlorophyll contents in the leaves and roots of A.pinnata were determined when the two-year seedlings were subjected to 0 (control), 7, 14, 21 d drought stress, and 3, 6 d re-watering. The results showed as follows: The SOD activities in leaves varied significantly under different treatments, and SOD activities in roots under drought stresses and re\|watering were significantly higher than those of the control. The contents of proline increased significantly under 21 d drought stress, and decreased drastically then. However, the contents of soluble protein in leaves and roots decreased significantly under 14 and 21 d drought stress, and increased drastically during re-watering process. MDA contents in leaves and roots showed the contrary change trends. The relative electrical conductivity in leaves and roots increased drastically under 14 and 21 d drought stresses, then decreased during re-watering process. The contents of chlorophyll increased and reached to the maximum under 21 d drought stress. The dynamic tendencies of proline content, soluable protein content and relative electrical conductivity in leaves were similar to those in roots. However, different change trends of SOD activities were observed in leaves and roots, which also for MDA contents. The physiological change trends indicated the synergistic effects in leaves and roots of A.pinnata to drought stress and re-watering process.

Key words: Arenga pinata, drought stress, re-watering, leaves, roots