浙江农业学报

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大豆异黄酮对去卵巢大鼠脾脏NGF、IL2蛋白表达的影响

  

  1. (1.四川农业大学 动物医学院,动物疾病与人类健康中心四川省重点实验室,四川 成都611130; 2.四川农业大学 动物医学院 实验动物疾病模型研究室,四川 成都611130; 3.四川农业大学 预防兽医研究所,四川 成都611130)
  • 出版日期:2016-07-25 发布日期:2016-07-08

Effect of soybean isoflavone on NGF, IL-2 protein in ovariectomized rats spleen

  1. (1. Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China; 2. Laboratory of Animal Disease Model, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China; 3. Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China)
  • Online:2016-07-25 Published:2016-07-08

摘要: 为了探讨大豆异黄酮(SI)对去卵巢大鼠脾脏中神经生长因子(NGF)、白细胞介素2(IL-2)蛋白表达的影响,本研究在成功建立去卵巢大鼠模型的基础上,对去卵巢大鼠分别隔天皮下注射高(1.5 mg·kg-1)、中(1.0 mg·kg-1)、低(0.5 mg·kg-1 )剂量的SI,假手术组(仅手术而不去卵巢)和溶媒组(去卵巢)大鼠隔天皮下注射溶媒剂(乙醇)。补充SI至14 d和42 d时,每组随机剖杀5只大鼠,采用免疫组织化学方法,对大鼠脾脏NGF、IL-2蛋白的表达变化进行研究。结果表明,脾脏中广泛分布着NGF、IL-2蛋白,主要分布于被膜下方的红髓区,在白髓分布量极少。去卵巢后,大鼠脾脏中NGF、IL-2蛋白的表达强度和阳性细胞数目均显著下降,而且随着去除卵巢时间的延长下降加剧。体外补充SI后14 d,各剂量组和42 d低、中剂量组仅有部分恢复,变化不显著;补充高剂量SI 42 d后,脾脏NGF、IL-2蛋白的表达强度和阳性细胞数目基本恢复至假手术组的水平。大豆异黄酮能上调NGF、IL-2蛋白在脾脏内的表达,并呈现时间和剂量的依赖性。

关键词: 大豆异黄酮, 免疫组化, NGF, IL-2, 脾脏

Abstract: To study the effect of soybean isoflavone on NGF, IL-2 protein in the ovariectomized rats spleen, young female SD rats were ovariectomized successfully, and then were supplied with soy isoflavone (SI) in high dosage (1.5 mg·kg-1), medium dosage (1.0 mg·kg-1), low dosage (0.5 mg·kg-1), respectively. The rats of sham operation group and the control group were supplied with solvent agent. All of the above groups endured hypodermic injection once every other day. After 14 and 42 d treatment, 5 rats per group were killed for study. The expression and distribution of NGF, IL-2 in spleen were studied by immunohistochemical methods. The NGF, IL-2 protein were mainly distributed in red pulp close to the capsule. The intensity and number of NGF, IL-2 positive cells of the ovariectomized rats were significantly decreased, and after ovariectomizing, the damage was enhanced with prolonged time. After soy isoflavone treatment, the intensity and number of NGF and IL2 positive cells of ovariectomized rats increased and showed a timedependent manner. During the same period, with the increased concentration of soy isoflavone, the intensity and number of NGF and IL-2 positive cells increased and showed a dosedependent manner. The above results suggested that soy isoflavone could upregulate the expression of NGF and IL2 in a timeand dosedependent manner.

Key words: soy isoflavone, immunohistochemical, NGF, IL-2, spleen