耐镉菌株N-17的分离、鉴定及其强化印度芥菜富集土壤镉的潜力

doi:10.3969/j.issn.1004-1524.2017.05.18

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (5): 812-817.DOI: 10.3969/j.issn.1004-1524.2017.05.18

耐镉菌株N-17的分离、鉴定及其强化印度芥菜富集土壤镉的潜力

杜东霞, 杜宇, 许隽, 尹红梅, 贺月林^*

湖南省微生物研究院,湖南长沙 410009

收稿日期:2017-01-09 出版日期:2017-05-20 发布日期:2017-06-06
通讯作者: 贺月林,E-mail: 719641546@qq.com
作者简介:杜东霞(1980-),女,山东荷泽人,硕士,助理研究员,主要从事农牧废弃物处理及农田土壤污染防控等方面的研究。E-mail: xiaxia414@126.com
基金资助:
湖南省自然科学基金项目 (13JJ2035); 农业部、财政部“长株潭”耕地重金属污染修复及农作物种植结构调整子项目(农办财函〔2016〕6号)

Isolation, identification of a Cd-resistant strain N-17 and its potential in enhancing cadmium accumulation by Brassica juncea

DU Dongxia, DU Yu, XU Jun, YIN Hongmei, HE Yuelin^*

Hunan Academy of Microbiology, Changsha 410009, China

Received:2017-01-09 Online:2017-05-20 Published:2017-06-06

摘要/Abstract

摘要： 从镉污染稻田土壤中分离到一株兼具活化碳酸镉、分泌铁载体和固氮能力的耐镉菌株N-17,结合菌体形态、生理生化及16S rDNA分析,初步鉴定其为伯克氏菌属。将其用于强化印度芥菜修复镉污染土壤,盆栽试验结果表明,该菌株能增加污染土壤中可交换态镉的含量,与对照相比,接种该菌后,印度芥菜的生物量提高了9.41%,株高增加了20.2%,地上部和地下部镉含量分别比对照提高了49%和67%。

关键词: 铁载体, 固氮, 伯克氏菌属, 植物修复

Abstract: A Cd-resistant strain N-17, with siderophore-producing, nitrogen-fixing and CdCO₃-solubilizing capacity, was isolated from paddy soil with Cd pollution. Combined with the colony morphology, physiological and biochemical characteristics and 16S rDNA sequence analysis, N-17 was identified as Burkholderia sp. A pot experiment was done to investigate the effect of N-17 on phytoremediation of Cd polluted soil by Brassica juncea. It was shown that this strain could significantly increase the content of exchangeable cadmium in contaminated soil. Compared with control, the biomass and plant height of Brassica juncea inoculated with N-17 was increased by 9.41% and 20.2%, respectively. The cadmium content in aboveground and root of Brassica juncea inoculated with N-17 was increased by 49% and 67%, respectively.

Key words: siderophore, nitrogen-fixing capacity, Burkholderia sp., phytoremediation

中图分类号:

X172

杜东霞, 杜宇, 许隽, 尹红梅, 贺月林. 耐镉菌株N-17的分离、鉴定及其强化印度芥菜富集土壤镉的潜力[J]. 浙江农业学报, 2017, 29(5): 812-817.

DU Dongxia, DU Yu, XU Jun, YIN Hongmei, HE Yuelin. Isolation, identification of a Cd-resistant strain N-17 and its potential in enhancing cadmium accumulation by Brassica juncea[J]. , 2017, 29(5): 812-817.

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耐镉菌株N-17的分离、鉴定及其强化印度芥菜富集土壤镉的潜力

Isolation, identification of a Cd-resistant strain N-17 and its potential in enhancing cadmium accumulation by Brassica juncea

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