浙江农业学报 ›› 2018, Vol. 30 ›› Issue (6): 926-931.DOI: 10.3969/j.issn.1004-1524.2018.06.06

• 动物科学 • 上一篇    下一篇

猪乙型脑炎病毒SCYA201201株感染小鼠后突破血脑屏障能力研究

柴春霞, 王侨, 赖雅兰, 徐伊璇, 文心田, 曹三杰, 黄小波, 文翼平, 赵勤, 伍锐*   

  1. 四川农业大学 动物医学院 猪病研究中心,四川 成都 611130
  • 收稿日期:2017-10-30 出版日期:2018-06-20 发布日期:2018-07-02
  • 通讯作者: 伍锐,E-mail: wurui1977@163.com
  • 作者简介:柴春霞(1993—),女,内蒙古呼和浩特人,硕士,主要从事猪乙型脑炎病毒研究。E-mail: chaichunxia_ccx@126.com
  • 基金资助:
    国家重点研发计划(2016YFD0500403)

Ability to break through blood-brain barrier of porcine Japanese encephalitis virus SCYA201021 strain after infection in mice

CHAI Chunxia, WANG Qiao, LAI Yalan, XU Yixuan, WEN Xintian, CAO Sanjie, HUANG Xiaobo, WEN Yiping, ZHAO Qin, WU Rui*   

  1. Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
  • Received:2017-10-30 Online:2018-06-20 Published:2018-07-02

摘要: 将猪乙型脑炎病毒SCYA201201株(F122代次,弱毒株)脑内接种小鼠,评估该病毒株对小鼠的致病性。通过腹腔接种小鼠,分别在接种后6、12、18、24 h观察小鼠脑部病理变化,同时利用荧光定量PCR方法检测其血液、脑组织E基因拷贝数,探究该毒株突破小鼠血脑屏障的能力,从而评估SCYA201201株(F122代次)作为弱毒活疫苗的潜力。结果显示,SCYA201201株(F122代次)高度弱化,对乳鼠的脑内半数致死量(LD50)仅为4×105 PFU·40 μL-1。该毒株腹腔接种24 h内,小鼠脑部未出现病理变化,且荧光定量PCR检测为阴性,表明该毒株不能突破小鼠的血脑屏障,具有很高的安全性。

关键词: 猪乙型脑炎病毒, 半数致死量, 荧光定量PCR, 血脑屏障

Abstract: In the present study, SPF Kuming mice were inoculated with SCYA201021 strain (F122 generation, attenuated strain of porcine Japanese encephalitis virus) by intracerebral injection in order to evaluate its potential value as the attenuated live vaccine, and its ability to break through the blood-brain barrier in mice. After infection in mice, pathological changes in mice brain were observed, and the E gene copes were measured by fluorescent quantitative PCR in the blood and brain at 6, 12, 18, 24 h after inoculation, respectively. The results indicated that the virulence of SCYA201021 strain (F122 generation) was significantly weakened as the half lethal does (LD50) in suckling mice brain was only 4×105 PFU·40 μL-1. Moreover, the mice did not show pathological changes in the brain during the 24 h after intraperitoneal injection, and the quantitative PCR results were negative, indicating that the SCYA201021 strain could not break through the blood-brain barrier in mice and the SCYA201021 strain had high safety.

Key words: porcine Japanese encephalitis virus, half lethal does, fluorescent quantitative PCR, blood-brain barrier

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