响应面法优化马铃薯茎段高效再生体系

doi:10.3969/j.issn.1004-1524.2018.06.05

浙江农业学报 ›› 2018, Vol. 30 ›› Issue (6): 918-925.DOI: 10.3969/j.issn.1004-1524.2018.06.05

响应面法优化马铃薯茎段高效再生体系

江林娟¹, 邹雪¹, 黄雪丽¹, 倪苏¹, 李立芹¹, 杨世民^{1, 2, *}

1.四川农业大学农学院,四川成都 611130;
2.四川农业大学作物科学国家级实验教学示范中心,四川成都 611130

收稿日期:2017-09-04 出版日期:2018-06-20 发布日期:2018-07-02
通讯作者: 杨世民,E-mail: yangshimin1@163.com
作者简介:江林娟(1985—),女,浙江嘉善人,硕士研究生,主要从事植物组织培养及遗传育种研究。E-mail: juan498@163.com
基金资助:
四川省科技创新苗子工程 (2015120); 国家重点研发计划子课题(2016YFD0300908-03)

Optimization of efficient regeneration system in stem of potato using response surface methodology

JIANG Linjuan¹, ZOU Xue¹, HUANG Xueli¹, NI Su¹, LI Liqin¹, YANG Shimin^{1, 2, *}

1. College of Agronomy, Sichuan Agricultural University, Chengdu 611130, China;
2.National Demonstration Center for Experimental Crop Science Education, Sichuan Agricultural University, Chengdu 611130, China

Received:2017-09-04 Online:2018-06-20 Published:2018-07-02

摘要/Abstract

摘要： 为了探讨马铃薯茎段愈伤组织高效再生的最佳激素浓度配比,建立马铃薯组织培养和快速繁殖技术新体系,以马铃薯品种川芋10号茎段为外植体诱导的愈伤组织为材料,通过采用响应面方法(RSM)中的Box-Behnken设计方法,研究TDZ、2,4-D、GA₃浓度对马铃薯茎段愈伤组织芽分化的影响,优化马铃薯愈伤组织芽分化的最佳激素配比。结果表明,从响应面法优化所得模型可知,培养基中的激素浓度变化对马铃薯愈伤组织出芽敏感性TDZ>GA₃>2,4-D;所得模型的决定系数R²为0.9921,确定了川芋10号茎段愈伤组织芽分化最佳培养基为MS+2.02 mg·L^-1 TDZ+0.08 mg·L^-1 2,4-D+2.25 mg·L^-1 GA₃,在此条件下,实际测得的平均出芽率与预测值基本一致,证实了该模型的有效性。

关键词: 马铃薯, 愈伤组织, 响应面法, 培养基

Abstract: To establish the optimal hormone concentrations of culture medium for efficient regeneration of potato stem callus tissue, as well as build a new system for tissue culture and rapid propagation technology, stem of Chuanyu No.10 was employed as explants to induce callus tissue, and the Box-Behnken design method of response surface methodology (RSM) was used to research the effect of TDZ, 2,4-D and GA₃ on the differentiation of potato callus. Meanwhile, the hormone concentrations were optimized. The results showed that the sensitivity of the hormone concentrations in culture medium to potato callus budding from the response surface method optimization model is TDZ>GA₃>2,4-D. The determination conefficient R² of the model was 0.992 1, the optimal medium for adventitious bud differentiation from stem callus of Chuanyu No.10 was MS+2.02 mg·L^-1 TDZ+0.08 mg·L^-1 2,4-D+2.25 mg·L^-1 GA₃.In this condition, the budding rate was actual measured, which was consistent with the predicted values. That confirmed the validity of the model.

Key words: potato, callus, response surface method, medium

中图分类号:

S532

江林娟, 邹雪, 黄雪丽, 倪苏, 李立芹, 杨世民. 响应面法优化马铃薯茎段高效再生体系[J]. 浙江农业学报, 2018, 30(6): 918-925.

JIANG Linjuan, ZOU Xue, HUANG Xueli, NI Su, LI Liqin, YANG Shimin. Optimization of efficient regeneration system in stem of potato using response surface methodology[J]. , 2018, 30(6): 918-925.

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响应面法优化马铃薯茎段高效再生体系

Optimization of efficient regeneration system in stem of potato using response surface methodology

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