黄淮白山羊肠道黏膜组织结构及杯状细胞分布特点

doi:10.3969/j.issn.1004-1524.2018.06.09

浙江农业学报 ›› 2018, Vol. 30 ›› Issue (6): 946-952.DOI: 10.3969/j.issn.1004-1524.2018.06.09

黄淮白山羊肠道黏膜组织结构及杯状细胞分布特点

周金星^{1, 2}, 钱兆全¹, 靳二辉¹, 刘文举², 金光明^{1, *}

1.安徽科技学院动物科学学院,安徽凤阳 233100;
2.安徽科技学院细胞与分子生物学研究中心,安徽凤阳 233100

收稿日期:2018-01-04 出版日期:2018-06-20 发布日期:2018-07-02
通讯作者: 金光明,E-mail: jingm@ahstu.edu.cn
作者简介:周金星(1976—),男,安徽五河人,博士,副教授,主要从事动物组织病理与细胞学研究。E-mail: zhoujx@ahstu.edu.cn
基金资助:
安徽省农业科技成果转化项目(1504032001); 安徽省科技攻关项目(1301042102); 安徽省高等学校自然科学研究重点项目(KJ2016A823); 安徽省第七批“115”产业创新团队项目(皖人才〔2014〕4号); 安徽科技学院重点建设学科项目(AKZDXK2015A04); 安徽省高校优秀青年骨干人才国内访学研修项目(gxfx2017078)

Mucous membrane organization structure characteristics and goblet cells distribution observation of Huanghuai white goat's intestinal tract

ZHOU Jinxing^{1, 2}, QIAN Zhaoquan¹, JIN Erhui¹, LIU Wenju², JIN Guangmin^{1, *}

1. College of Animal Science, Anhui Science and Technology University, Fengyang 233100, China;
2. Cell and Molecular Biology Research Center, Anhui Science and Technology University, Fengyang 233100, China

Received:2018-01-04 Online:2018-06-20 Published:2018-07-02

摘要/Abstract

摘要： 为探明黄淮白山羊肠道黏膜组织结构和杯状细胞分布特点,取成年健康黄淮白山羊3头,经麻醉后颈静脉放血致死,立即取小肠和大肠各3段,投入到4%多聚甲醛固定液中固定72 h,制作组织切片,经HE和PAS染色后,观察黄淮白山羊小肠和大肠黏膜组织结构和杯状细胞分布特点。结果表明,黄淮白山羊十二指肠和空肠的上皮细胞呈高柱状,固有层分布有较多的淋巴细胞,回肠黏膜的上皮细胞呈低柱状,黏膜下层中含大量的集合淋巴小结,盲肠、结肠和直肠的上皮细胞呈低柱状,大肠腺发达,腺体间弥散着淋巴细胞,直肠的固有层中可见淋巴小结,上皮之间的杯状细胞呈顶浆分泌的形式分泌黏多糖。黄淮白山羊小肠杯状细胞分泌的黏多糖以中性为主,大肠杯状细胞分泌的黏多糖以酸性为主。大肠三段杯状细胞数量极显著(P<0.01)高于小肠杯状细胞,大肠中结肠杯状细胞数量显著(P<0.05)高于盲肠和直肠,小肠中回肠杯状细胞数量显著(P<0.05)高于十二指肠和空肠。因此,黄淮白山羊从十二指肠到回肠,上皮细胞由高柱状逐渐向低柱状变化,杯状细胞主要分布于大肠,在结肠中数量最多。

关键词: 杯状细胞, 组织结构, 小肠, 大肠, 黄淮白山羊

Abstract: To observe mucous membrane organization structure characteristics and goblet cells distribution of Huanghuai white goat's intestinal tract, three adult healthy Huanghuai white goats were bleed from jugular vein to death, the samples of duodenum, jejunum, ileum, cecum, colon and rectum were taken immediately and immersed into 4% paraformaldehyde fixative to be fixed for 72 h. Tissue slices were thus made, and stained with HE and PAS. The results showed that epithelial cells of the duodenum and jejunum of Huanghuai white goat were highly columnar, and lymphocytes were distributed in lamina propria. The epithelial cells of the ileum mucosa were low columnar, and a large collection of lymph nodes were distributed in submucosa. The epithelial cells of cecum, colon and rectum were also low columnar, large intestinal gland were developed, and there were diffused lymphocytes between glands. The lymphoid nodule was seen in the lamina propria of rectum. The goblet cells in the epithelium secreted mucopolysaccharide in the form of apical secretion. The small intestinal goblet cells of Huanghuai white goat secreted neutral-mucopolysaccharide, while the large intestinal goblet cells of Huanghuai white goat secreted acid-mucopolysaccharide. The goblet cell's number of large intestine was significantly (P<0.01) higher than that of small intestine. The goblet cell number of colon were significantly (P<0.05) higher than those of the cecum and rectum, and the goblet cell number of ileum were significantly(P<0.05) higher than those of the duodenum and jejunum. Therefore, from duodenum to ileum, the epithelial cells gradually changed from high column to low column, the goblet cells were mainly distributed in the large intestine, and the highest number of goblet cells was found in the colon.

Key words: goblet cells, tissue structure, small intestine, large intestine, Huanghuai white goat

中图分类号:

S852.16

周金星, 钱兆全, 靳二辉, 刘文举, 金光明. 黄淮白山羊肠道黏膜组织结构及杯状细胞分布特点[J]. 浙江农业学报, 2018, 30(6): 946-952.

ZHOU Jinxing, QIAN Zhaoquan, JIN Erhui, LIU Wenju, JIN Guangmin. Mucous membrane organization structure characteristics and goblet cells distribution observation of Huanghuai white goat's intestinal tract[J]. , 2018, 30(6): 946-952.

参考文献

[1] 朱继开,钟煜,王璐璇,等. 肠道杯状细胞与黏蛋白的研究进展[J]. 继续医学教育,2017,31(7):139-140.
ZHU J K, ZHONG Y, WANG L X, et al.Research progress of intestinal goblet cells and mucin[J]. Continuing Medical Education, 2017, 31(7):139-140. (in Chinese with English abstract)
[2] 李冰,于岩波. 肠黏液屏障在肠道中的作用[J]. 世界华人消化杂志,2017,25(19):1764-1771.
LI B, YU Y B.Role of mucus barrier in the gut[J]. World Chinese Journal of Digestology, 2017, 25(19):1764-1771. (in Chinese with English abstract)
[3] LUU Y, JUNKER W, RACHAGANI S, et al.Human intestinal MUC17 mucin augments intestinal cell restitution and enhances healing of experimental colitis[J]. International Journal of Biochemistry & Cell Biology, 2010, 42(6):996-1006.
[4] 陈付菊, 陈耀星, 王子旭, 等. 新生犊牛小肠黏膜结构的早期发育及上皮内淋巴细胞和杯状细胞的数量变化[J]. 中国兽医科学, 2007, 37(6): 519-523.
CHEN F J, CHEN Y X, WANG Z X, et al.Early development of mucosal structure and quantitative changes of intraepithelial lymphocyte and goblet cell in the small intestine of neonatal calf[J]. Veterinary Science in China, 2007, 37(6):519-523. (in Chinese with English abstract)
[5] 田波, 李栋, 方梅, 等. 牦牛大肠组织结构及黏膜免疫相关细胞的分布[J]. 中国兽医学报, 2014, 34(12):1989-1995.
TIAN B, LI D, FANG M, et al.Observation on the distribution of mucosal immunity-associated cells and micro-structure of yak large intestine[J]. Chinese Journal of Veterinary Science, 2014, 34(12):1989-1995. (in Chinese with English abstract)
[6] 马兰, 常兰. 不同海拔藏绵羊小肠上皮内淋巴细胞、杯状细胞的数量和分布比较及其意义[J]. 青海医学院学报, 2012, 33(2):99-102.
MA L, CHANG L.Comparative study and significance discussion of quantity and distribution on small intestine intraepithelial lymphocytes and goblet cells between Tibetan sheep at different elevation regions[J]. Journal of Qinghai Medical College, 2012, 33(2):99-102. (in Chinese with English abstract)
[7] 刘小龙, 范寰, 陈龙宾, 等. 天蚕素和合生素对AA肉鸡小肠黏膜形态和小肠免疫细胞的影响[J]. 中国畜牧兽医, 2017, 44(11):3187-3194.
LIU X L, FAN H, CHEN L B, et al.Effects of cecropin and synbiotics on the intestinal mucosal morphology and intestinal mucosal immune cells of AA broilers[J]. China Animal Husbandry & Veterinary Medicine, 2017, 44(11):3187-3194. (in Chinese with English abstract)
[8] 唐秀莹, 陈正礼, 罗启慧, 等. 大豆异黄酮对大鼠肠道上皮内淋巴细胞、杯状细胞及瘦素长型受体的影响[J]. 浙江大学学报(农业与生命科学版), 2013, 39(3):343-350.
TANG X Y, CHEN Z L, LUO Q H, et al.Effect of soy isoflavones on intraepithelial lymphocytes, goblet cells and long leptin receptor in intestinal of rats[J]. Journal of Zhejiang University (Agriculture & Life Sciences.), 2013, 39(3):343-350. (in Chinese with English abstract)
[9] 张美曦, 隋欣, 刘超男, 等. 双歧杆菌对雏鸡肠道杯状细胞数量及黏蛋白2含量的影响[J]. 中国兽医杂志, 2016, 52(12):24-28.
ZHANG M X, SUI X, LIU C N, et al.Effects of bifidobacterium to the number of goblet cells and the content of MUC2 in intestinal tract of chicks[J]. Chinese Journal of Veterinary Medicine, 2016, 52(12):24-28. (in Chinese with English abstract)
[10] 邢亚丽, 宋慧, 姜云瑶, 等. 树舌发酵浸膏多糖对肉鸡肠黏膜组织结构及肠道免疫细胞的影响[J]. 中国农业大学学报, 2015, 20(3):126-132.
XING Y L, SONG H, JIANG Y Y, et al.Effects of the polysaccharides from the fermentation extract of Ganoderma applanatum on the mucosal structure and intestinal immunocyte of broilers[J]. Journal of China Agricultural University, 2015, 20(3):126-132. (in Chinese with English abstract)
[11] 闫晓刚, 张芳毓, 郎洪彦, 等. 人参茎叶多糖对雏鸡小肠黏膜组织发育和免疫细胞的影响[J]. 畜牧兽医学报, 2015, 46(1):144-155.
YAN X G, ZHANG F Y, LANG H Y, et al.Effects of ginseng stem and leaf polysaccharide on small intestinal mucous tissue development and immune cells of chicken[J]. Acta Veterinaria et Zootechnica Sinica, 2015, 46(1):144-155. (in Chinese with English abstract)
[12] 曹贵彬, 易凤珍, 游金明, 等. 陈皮苷制剂对爱拨益加肉鸡生长性能、免疫器官指数及小肠黏膜形态结构的影响[J]. 动物营养学报, 2014, 26(3):710-717.
CAO G B, YI F Z, YOU J M, et al.Effects of hesperidin preparation on growth performance, immune organ indexes and small intestinal mucosal morphology of arbor acres broilers[J]. Chinese Journal of Animal Nutrition, 2014, 26(3):710-717. (in Chinese with English abstract)
[13] 周金星, 盛洁, 高文, 等. 黄淮白山羊兴盛期毛囊和皮肤组织结构的观察[J]. 中国兽医科学, 2017, 47(6): 779-784.
ZHOU J X, SHENG J, GAO W, et al.Observation of tissue structure characteristics of the follicle and skin of Huanghuai white goat in a prosperous period[J]. Chinese Veterinary Science, 2017, 47(6):779-784. (in Chinese with English abstract)
[14] 周金星, 盛洁, 高文, 等. 黄淮白山羊蹄部形态结构的观察[J]. 中国兽医科学, 2017, 47(5):655-659.
ZHOU J X, SHENG J, GAO W, et al.Observation of morphological structures of the hoof in Huanghuai white goat[J]. Chinese Veterinary Science, 2017, 47(5):655-659. (in Chinese with English abstract)
[15] 周金星, 靳二辉, 胡倩倩, 等. 黄淮白山羊冠状动脉和心肌组织结构及CX-43蛋白的分布观察[J]. 中国兽医科学, 2016, 46(8):1031-1037.
ZHOU J X, JIN E H, HU Q Q, et al.Characteristic observation of coronary artery, myocardial tissue structure and CX-43protein distribution in Huanghuai white goat[J]. Chinese Veterinary Science, 2016, 46(8):1031-1037. (in Chinese with English abstract)
[16] 华金玲, 郭亮, 王立克, 等. 不同精粗比日粮对黄淮白山羊瘤胃内环境的影响[J]. 中国兽医学报, 2013, 33(6):913-917.
HUA J L, GUO L, WANG L K, et al.The effect of forage to concentrate ratio on ruminal parameters in Huanghuai white goat[J]. Chinese Journal of Veterinary Science, 2013, 33(6):913-917. (in Chinese with English abstract)
[17] 华金玲, 郭亮, 王立克, 等. 不同精粗比日粮对黄淮白山羊瘤胃挥发性脂肪酸影响[J]. 东北农业大学学报, 2013, 44(6):58-61.
HUA J L, GUO L, WANG L K, et al.Effect of different forage to concentrate ratios on VFA of Huanghuai white goat[J]. Journal of Northeast Agricultural University, 2013, 44(6):58-61. (in Chinese with English abstract)
[18] 李斌, 韩伟, 方俊顺, 等. 黄淮白山羊孤雌胚胎的体外培养[J]. 中国兽医学报, 2008, 28(12):1480-1482.
LI B, HAN W, FANG J S, et al.Development of Huanghuai-white goat parthenogenetic embryos in vitro[J]. Chinese Journal of Veterinary Science, 2008, 28(12):1480-1482. (in Chinese with English abstract)
[19] 来景辉, 范红杰. 安徽省黄淮白山羊衣原体病的血清学调查[J]. 中国农学通报, 2014, 30(11):26-28.
LAI J H, FAN H J.Serological investigation of chlamydiosis in Huanghuai-white goats in Anhui province[J]. Chinese Agricultural Science Bulletin, 2014, 30(11):26-28. (in Chinese with English abstract)
[20] 陈珍, 周作红, 管远红, 等. 60日龄内玉山黑猪小肠形态学发育的研究[J]. 江西农业大学学报, 2008, 30(4):297-302.
CHEN Z, ZHOU Z H, GUAN Y H, et al.A study on the tissue morphological development of small intestine of Yushan black piglets in 60 day-age[J]. Acta Agriculturae Universitatis Jiangxiensis, 2008, 30(4):297-302. (in Chinese with English abstract)
[21] 闫金坤, 陈耀星, 王子旭. 雌激素对大鼠肠黏膜结构及上皮内淋巴细胞和杯状细胞数量、分布的影响[J]. 畜牧兽医学报, 2008, 39(9):1267-1271.
YAN J K, CHEN Y X, WANG Z X.Effect of estrogen on structure of mucosal epithelium and number of intraepithelial lymphocytes and goblet cells in small intestine of rat[J]. Acta Veterinaria et Zootechnica Sinica, 2008, 39(9):1267-1271. (in Chinese with English abstract)
[22] WU X, RUAN Z, CAO Y, et al.Dietary supplementation with L-arginine or N-carbamylglutamate enhances intestinal growth and heat shock protein-70 expression in weanling pigs fed a corn and soybean meal-based diet[J]. Amino Acids, 2010, 39(3):831-839.
[23] 刘玥宏, 丁悦, 徐敬东. 肠道杯状细胞及其功能研究进展[J]. 世界华人消化杂志, 2017, 25(14):1279-1286.
LIU Y H, DING Y, XU J D. Goblet cells and their functions[J]. World Chinese Journal of Digestology, 2017, 25(14):1279-1286. (in Chinese with English abstract)

黄淮白山羊肠道黏膜组织结构及杯状细胞分布特点

Mucous membrane organization structure characteristics and goblet cells distribution observation of Huanghuai white goat's intestinal tract

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	刘磊, 王讯, 罗毅, 梁继元, 颜培祺, 李昕怡, 刘薇, 刘辰恺, 李佳佳. 鸽小肠上皮细胞的分离培养与鉴定[J]. 浙江农业学报, 2021, 33(2): 239-247.
[2]	杨华, 马艳, 刘秀婷, 吕文涛, 卢立志, 肖英平. 即食鸭制品大肠埃希菌污染状况、毒力基因和耐药特征分析[J]. 浙江农业学报, 2020, 32(10): 1841-1848.
[3]	常江, 罗怡, 唐标, 张玲, 戴贤君, 裘罕琦, 杨华, 夏效东. 一株分离自鸡肉样品的多重耐药大肠埃希菌的全基因组测序及耐药性研究[J]. 浙江农业学报, 2019, 31(8): 1249-1256.
[4]	彭成璐, 张瑜, 丁雪东, 李玉, 冯士彬, 王希春, 李锦春, 吴金节. 11S通过NF-κB信号通路引起猪小肠上皮细胞损伤[J]. 浙江农业学报, 2019, 31(3): 384-391.
[5]	彭成璐, 张瑜, 夏晓冬, 贺濛初, 舒迎霜, 冯士彬, 李玉, 王希春, 李锦春, 吴金节. β-伴大豆球蛋白通过p38/JNK MAPK信号通路引起IPEC-J2细胞损伤[J]. 浙江农业学报, 2019, 31(2): 242-249.
[6]	彭珂楠, 周雪珂, 殷鑫欢, 李飞, 蔡瑶, 曾喻兵, 江朝源, 张儒博, 杨泽晓, 徐志文, 朱玲. 四川地区猪源致病性大肠埃希菌分子分群、生物被膜形成能力及耐药性研究[J]. 浙江农业学报, 2019, 31(10): 1599-1607.
[7]	周金星, 钱兆全, 靳二辉, 刘文举, 金光明. 复方中药制剂对SD大鼠胸腺组织结构及Caspase-3蛋白分布的影响[J]. 浙江农业学报, 2018, 30(5): 722-729.
[8]	刘翠艳, 陈四玉, 孙桃桃, 尚莉莉, 杨明川, 杨孜生, 韩春杨. 马齿苋-益生菌联用对热毒证小鼠腹泻的预防效果[J]. 浙江农业学报, 2018, 30(2): 211-219.
[9]	王希春, 张娅菲, 储小燕, 朱雷, 朱电锋, 冯士彬, 李玉, 吴金节. DON暴露对断奶仔猪抗氧化水平、血清生化指标及小肠黏膜形态的影响[J]. 浙江农业学报, 2018, 30(10): 1655-1661.
[10]	杨宗英, 张一柳, 胡鲲, 杨先乐, 刘力硕, 张凤翔, 蔡红桂. 溴氰菊酯对中华绒螯蟹肝胰腺氧化胁迫效应和组织结构的影响[J]. 浙江农业学报, 2017, 29(8): 1261-1270.
[11]	杨华, 何祥祥, 肖英平, 钱鸣蓉, 张巧艳, 唐标. 浙江省三地区冷鲜鸡中大肠埃希菌耐药谱测定及MLST分子分型分析[J]. 浙江农业学报, 2017, 29(6): 888-893.
[12]	黄书伦, 郑凯文, 胡洋, 李章程, 程方俊, 宋振辉, 周作勇, 刘国林, 林春发, 林永润, 张婷婷. 鸭源大肠杆菌Ⅰ型菌毛的鉴定及FimA、FimH基因特征分析[J]. 浙江农业学报, 2017, 29(12): 1994-1999.
[13]	靳二辉, 周金星, 任曼, 胡倩倩, 金光明, 李升和. 酵母硒和硼联合添加对肉鸡免疫器官组织结构及免疫功能的影响[J]. 浙江农业学报, 2017, 29(11): 1783-1795.
[14]	刘勇亮, 陈波, 刘明刚, 徐毓琴, 戴茜茜, 谷笑笑, 潘康成. 连梅止痢中药制剂对大肠埃希菌K88感染仔猪的疗效[J]. 浙江农业学报, 2016, 28(9): 1493-1500.
[15]	陈秀萍，肖正润，林晶，汪燕秋，张洁，黄启发，俞道进*. 氮磷营养盐对大肠杆菌四环素耐药性的影响[J]. 浙江农业学报, 2016, 28(2): 247-.