台州猕猴桃溃疡病病原菌分子鉴定及药剂筛选

doi:10.3969/j.issn.1004-1524.2018.09.15

浙江农业学报 ›› 2018, Vol. 30 ›› Issue (9): 1548-1554.DOI: 10.3969/j.issn.1004-1524.2018.09.15

台州猕猴桃溃疡病病原菌分子鉴定及药剂筛选

钟永军^{1, 2}, 何昕蔚^{2, *}, 余达勇², 蒋晶晶², 江景勇³

1.中国科学院宁波材料技术与工程研究所功能材料与纳米器件事业部,浙江宁波 315201;
2.台州学院生命科学学院,浙江台州 318000;
3.台州市农业科学研究院,浙江临海 317000

收稿日期:2017-12-18 出版日期:2018-09-25 发布日期:2018-10-15
作者简介:钟永军(1986—),男,浙江嘉兴人,博士,讲师,从事病原微生物与生物技术药物研究。E-mail: yjzhong@tzc.edu.cn

Molecular identification of kiwifruit canker pathogen in Taizhou and screening of its control agents

ZHONG Yongjun^{1, 2}, HE Xinwei^{2, *}, YU Dayong², JIANG Jingjing², JIANG Jingyong³

1. Functional Materials and Nanodevices, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo 315201, China;
2. School of Life Sciences, Taizhou University, Taizhou 318000, China;
3. Taizhou Academy of Agricultural Sciences, Linhai 317000, China

Received:2017-12-18 Online:2018-09-25 Published:2018-10-15
Contact: 何昕蔚,E-mail: 710166263@qq.com
Supported by:
浙江省自然科学基金青年基金(LQ18D050001); 台州市科技计划(1701ny06,14NY02); 台州市大学生科技创新项目

摘要/Abstract

摘要： 为了针对性地防治猕猴桃细菌性溃疡病,采用平板涂布分离法从台州主要猕猴桃种植果园分离到1株丁香假单胞猕猴桃致病变种(Pseudomonas syringae pv. actinidae,Psa)。用生物型(biovar)特异性引物检测发现,试验分离到的Psa菌株是biovar 3型。通过抑菌圈大小比较,对23种农用杀菌剂进行了筛选。结果表明,不同农用杀菌剂对Psa菌株的抑菌效果相差很大,且以四环素、乙蒜素和丁子香酚的抑菌效果较好(抑菌圈直径≥15 mm)。此外,用最小抑菌浓度(MIC)和时间-杀菌试验研究了纳米氧化锌对Psa菌株的杀菌效果。结果显示,纳米氧化锌的MIC值为50 μg·mL^-1,且杀菌效果随纳米氧化锌的浓度和作用时间的增加而增强。

关键词: 猕猴桃细菌性溃疡病, 丁香假单胞猕猴桃致病变种, 生物型, 药敏试验, 纳米氧化锌

Abstract: To control the bacterial canker disease, a strain of Pseudomonas syringae pv. actinidae (Psa) from main kiwifruit planting orchards in Taizhou was isolated by using streak plate method in this study. Biovar specific primer detection results showed that the Psa strain was biovar 3. Through the comparison of antibacterial rings, 23 fungicides were examined. The results showed that the antibacterial effect of different fungicides on the Psa strain was quite different, and tetracycline, ethylicin and eugenol exhibited a strong antibacterial effect (the diameter of inhibitory ring≥15 mm). In addition, the antibacterial effect of nano zinc oxide (nano-ZnO) on the Psa strain was examined by minimum inhibitory concentration (MIC) and in vitro time-kill experiments. The results showed that the MIC value of nano-ZnO was 50 μg·mL^-1, and the antibacterial potential increased with the concentration of nano-ZnO and the time of action.

Key words: kiwifruit bacterial canker, Pseudomonas syringae pv. actinidae, biovar, drug sensitivity test, nano zinc oxide

中图分类号:

S436.634

钟永军, 何昕蔚, 余达勇, 蒋晶晶, 江景勇. 台州猕猴桃溃疡病病原菌分子鉴定及药剂筛选[J]. 浙江农业学报, 2018, 30(9): 1548-1554.

ZHONG Yongjun, HE Xinwei, YU Dayong, JIANG Jingjing, JIANG Jingyong. Molecular identification of kiwifruit canker pathogen in Taizhou and screening of its control agents[J]. , 2018, 30(9): 1548-1554.

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台州猕猴桃溃疡病病原菌分子鉴定及药剂筛选

Molecular identification of kiwifruit canker pathogen in Taizhou and screening of its control agents

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