静原鸡ELOVL5基因遗传多样性研究

doi:10.3969/j.issn.1004-1524.2019.02.04

浙江农业学报 ›› 2019, Vol. 31 ›› Issue (2): 200-206.DOI: 10.3969/j.issn.1004-1524.2019.02.04

静原鸡ELOVL5基因遗传多样性研究

张娟¹, 母童¹, 赵平², 陈佳萍¹, 冯小芳¹, 郭鹏¹, 武泽文¹, 刘丽元¹, 蒋秋斐¹, 顾亚玲^{1, *}

1.宁夏大学农学院,宁夏银川 750021;
2.彭阳县畜牧技术推广服务中心,宁夏固原 756500

收稿日期:2018-05-29 出版日期:2019-02-25 发布日期:2019-03-06
通讯作者: 顾亚玲,E-mail: guyaling@sina.com
作者简介:张娟(1982—),女,宁夏中卫人,博士,副教授,主要从事畜禽遗传育种与繁殖。E-mail: zhangjkathy@126.com
基金资助:
国家自然科学基金(31860621)

Polymorphism of ELOVL5 gene in Jingyuan chicken

ZHANG Juan¹, MU Tong¹, ZHAO Ping², CHEN Jiaping¹, FENG Xiaofang¹, GUO Peng¹, WU Zewen¹, LIU Liyuan¹, JIANG Qiufei¹, GU Yaling^{1, *}

1. College of Agriculture, Ningxia University, Yinchuan 750021, China;
2. Animal Husbandry and Technology Promotion Service Center of Pengyang County, Guyuan 756500, China

Received:2018-05-29 Online:2019-02-25 Published:2019-03-06

摘要/Abstract

摘要： 为确定静原鸡ELOVL5基因遗传变异位点,探讨其遗传特性,本研究利用单链构象多态性分析(PCR-SSCP)及DNA测序技术对静原鸡ELOVL5基因进行多态性检测。结果表明,静原鸡ELOVL5基因exon2和exon5均无多态性;在intron2扩增片段上共检测到1个SNP位点(g.88620433+1683G>A),其表现为2种基因型(AA、AG)。其中AA为优势基因型,基因型频率为0.84,A为优势等位基因,基因频率为0.92。在248个静原鸡群体中未发现纯合GG型个体。群体遗传学分析表明,该位点纯合度较高(0.84),PIC为0.14(PIC<0.25),呈低度多态。卡方适合性检验结果表明,静原鸡ELOVL5基因intron2突变未偏离Hardy-Weinberg平衡(P>0.05)。综上,静原鸡ELOVL5基因intron2多态性较低,exon2和exon5未发生变异。

关键词: 静原鸡, ELOVL5基因, SNP, 多态性

Abstract: Nucleotide polymorphism and site genetic characteristics of ELOVL5 gene in Jingyuan chicken were studied. Polymorphisms of ELOVL5 gene were detected by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and DNA sequence. The results showed that the ELOVL5 gene exon2 and exon5 had no polymorphism. The total of 1 SNP (g.88620433+1683G>A), 2 genotypes were detected on the intron2 amplified fragment of ELOVL5 gene (AA, AG), genotype AA and allele A was dominant, the frequency was 0.84 and 0.92. No homozygous GG type individual was found in 248 populations of Jingyuan chicken. Population genetic analysis showed that the homozygosity of the locus was higher (0.84) and PIC was 0.14 (PIC<0.25), showing low polymorphism. The results showed that the intron2 mutation of ELOVL5 gene in Jingyuan chicken did not deviate from Hardy-Weinberg equilibrium by χ² test (P>0.05). To sum up, the amplified fragment of the intron2 gene of Jingyuan chicken had low polymorphism, and there was no mutation in exon2 and exon5.

Key words: Jingyuan chicken, ELOVL5 gene, SNP, polymorphism

中图分类号:

S831

张娟, 母童, 赵平, 陈佳萍, 冯小芳, 郭鹏, 武泽文, 刘丽元, 蒋秋斐, 顾亚玲. 静原鸡ELOVL5基因遗传多样性研究[J]. 浙江农业学报, 2019, 31(2): 200-206.

ZHANG Juan, MU Tong, ZHAO Ping, CHEN Jiaping, FENG Xiaofang, GUO Peng, WU Zewen, LIU Liyuan, JIANG Qiufei, GU Yaling. Polymorphism of ELOVL5 gene in Jingyuan chicken[J]. , 2019, 31(2): 200-206.

参考文献

[1] 顾亚玲, 张娟, 何生虎.静原鸡品种选育与饲养管理[M].银川:阳光出版社,2017:4-5.
[2] 姜琳琳, 刘华贵, 齐德生,等. 鸡肉中脂肪酸的研究进展[J]. 肉类研究, 2006(1):37-40.
JIANG L L, LIU H G, QI D S, et al. Review on fatty acid in muscle tissues of chicken[J]. Meat Research, 2006(1):37-40. (in Chinese with English abstract)
[3] 母童, 顾亚玲, 赵平,等. 静原鸡ELOVL2基因遗传多态性研究[J]. 中国畜牧兽医, 2018, 45(3):730-737.
MU T, GU Y L, ZHAO P, et al.Study on polymorphism of ELOVL2 gene in Jingyuan chicken[J]. China Animal Husbandry & Veterinary Medicine, 2018, 45(3):730-737. (in Chinese with English abstract)
[4] NAGANUMA T, SATO Y, SASSA T, et al.Biochemical characterization of the very long-chain fatty acid elongase ELOVL7[J]. FEBS Letters, 2011, 585(20):3337-3341.
[5] 唐慧, 潘志雄, 卢立志,等. 超长链脂肪酸延伸酶家族的功能及表达调控[J]. 生命的化学, 2009, 29(6):898-901.
TANG H, PAN Z X, LU L Z, et al.The function and expression regulation of elongases of very long chain fatty acids[J]. Chemistry of Life, 2009, 29(6):898-901. (in Chinese with English abstract)
[6] SHIKAMA A, SHINOZAKI H, TAKEUCHI Y, et al.Identification of human ELOVL5 enhancer regions controlled by SREBP[J]. Biochemical & Biophysical Research Communications, 2015, 465(4):857-863.
[7] QIN Y, DALEN K T, GUSTAFSSON J Å, et al.Regulation of hepatic fatty acid elongase 5 by LXRα-SREBP-1c[J]. Biochimica et Biophysica Acta, 2009, 1791(2):140-147.
[8] WANG Y, TORRESGONZALEZ M, TRIPATHY S, et al.Elevated hepatic fatty acid elongase-5 activity affects multiple pathways controlling hepatic lipid and carbohydrate composition[J]. Journal of Lipid Research, 2008, 49(7):1538-1552.
[9] 王海燕, 苏玉虹. 编码极长链脂肪酸延长酶基因家族的结构及其产物的功能[J]. 生命的化学, 2005, 25(1):29-31.
WANG H Y, SU Y H.The structure and function of the coding long chain fatty acid gene family[J].Chemistry of Life,2005,25(1):29-30. (in Chinese)
[10] 邵亚明, 罗发兴, 田泽维,等.慢性乙型肝炎基因治疗若干进展[J]. 生命的化学, 2005, 25(1):31-33.
SHAO Y M,LUO F X,TIAN Z W, et al.Advances in gene therapy of chronic hepatitis B[J]. Chemistry of Life, 2005, 25(1):31-33. (in Chinese)
[11] 母童, 张娟, 赵平,等. 静原鸡ELOVL2和ELOVL5基因表达的组织特异性研究[J]. 浙江农业学报, 2017, 29(8):1290-1296.
MU T, ZHANG J, ZHAO P, et al.Tissue-specific expression analysis of ELOVL2 and ELOVL5 genes in Jingyuan chicken[J]. Acta Agriculturae Zhejiangensis, 2017, 29(8):1290-1296. (in Chinese with English abstract)
[12] MATSUMOTO H, SHIMIZU Y, TANAKA A, et al.The SNP in the promoter region of the bovine ELOVL5 gene influences economic traits including subcutaneous fat thickness[J]. Molecular Biology Reports, 2013, 40(4):3231-3237.
[13] 郭鹏程, 戴珊珊, 杨浩然,等. ELOVL5基因遗传变异与中国荷斯坦奶牛乳质性状的关联分析及功能验证[J]. 中国兽医学报, 2017, 37(4):741-745.
GUO P C, DAI S S, YANG H R, et al.ELOVL5 gene variants reveal significant effects of milk quality in Holstein cows[J].Chinese Journal of Veterinary Science, 2017,37(4):741-745. (in Chinese with English abstract)
[14] XIANG L I, GAN Z W, DING Z, et al.Genetic variants in the ELOVL5 but not ELOVL2 gene associated with polyunsaturated fatty acids in han chinese breast milk[J]. Biomedical and Environmental Sciences, 2017, 30(1):64-67.
[15] ZUO R, MAI K, XU W, et al.Molecular cloning, tissue distribution and nutritional regulation of a fatty acyl ELOVL5-like elongase in large yellow croaker, Larimichthys crocea[J]. Aquaculture Research, 2016, 47(8):2393-2406.
[16] CARMONA-ANTONANZAS G, ZHENG X, TOCHER D R, et al.Regulatory divergence of homeologous atlantic salmon ELOVL5 genes following the salmonid-specific whole-genome duplication[J]. Gene, 2016, 591(1):34-42.
[17] 吴景, 郑先虎, 匡友谊,等. 镜鲤脂肪酸延长酶5基因的克隆和表达分析[J]. 中国水产科学, 2015, 22(1):9-16.
WU J, ZHENG X H, KUANG Y Y, et al.Cloning and characteristic analysis of fatty acid elongase 5 in mirror carp (Cyprinus carpio)[J]. Journal of Fishery Sciences of China, 2015, 22(1):9-16. (in Chinese with English abstract)
[18] 覃川杰, 文正勇, 袁登越,等. 瓦氏黄颡鱼(Pelteobagrus vachellii)脂肪酸去饱和酶FAD2和延伸酶ELOVL5的克隆及表达分析[J]. 海洋与湖沼, 2017, 48(4):884-893.
QIN C J, WEN Z Y, YUAN D Y, et al.Molecular cloning and expression of fatty acid desaturase and elongase genes in darkbarbel catfish Pelteobagrus vachellii[J]. Oceanologia Et Limnologia Sinica, 2017, 48(4):884-893. (in Chinese with English abstract)
[19] 谭小音, 金丽, 谢德琼,等. 八眉猪SLA-DRA基因第4外显子多态性分析[J]. 生物技术, 2015, 25(5):463-467.
TAN X Y, JIN L, XIE D Q, et al.Polymorphism of SLA-DRA exon 4 in Bamei pigs[J]. Biotechnology, 2015, 25(5):463-467. (in Chinese with English abstract)
[20] REN H R, YU J, XU P, et al.Single nucleotide polymorphisms of Î6-desaturase and ELOVL5 segments and their associations with common carp (Cyprinus carpio) growth traits[J]. Genetics & Molecular Research, 2015, 14(4):12848-12854.
[21] SHI H B, DU Y, ZHANG C H, et al.Fatty acid elongase 5 (ELOVL5) alters the synthesis of long-chain unsaturated fatty acids in goat mammary epithelial cells[J]. Journal of Dairy Science, 2018, 101(5):4586-4587.
[22] ZHANG M, LI C C, LI F, et al. Estrogen promotes hepatic synthesis of long-chain polyunsaturated fatty acids by regulating ELOVL5 at post-transcriptional level in laying hens[J]. International Journal of Molecular Sciences, 2017, 18(7): pii: E1405.

静原鸡ELOVL5基因遗传多样性研究

Polymorphism of ELOVL5 gene in Jingyuan chicken

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	陈文强, 汪小福, 陈笑芸, 彭城, 徐俊锋, 蔡健. 基于ITS2和SNP技术鉴定浙江铁皮石斛的初步研究[J]. 浙江农业学报, 2021, 33(1): 69-76.
[2]	廖朝美, 游敏芳, 谭光辉, 李杰章, 张依裕, 覃媛钰, 刘若余. 三穗鸭PRKCB基因内含子14的变异对蛋壳品质的影响[J]. 浙江农业学报, 2020, 32(9): 1574-1580.
[3]	刘新雨, 田洁. 大蒜转录组简单重复序列标记分析与分子标记开发[J]. 浙江农业学报, 2020, 32(9): 1615-1625.
[4]	喻宗岗, 蒋隽, 姚亚铃, 郭英, 李闯, 燕海峰. 雪峰乌骨鸡HSP70基因多态性与精液品质关联分析[J]. 浙江农业学报, 2020, 32(8): 1378-1384.
[5]	吴佳, 陈朗, 姜涛, 黄国明, 李倬, 李耀东, 张丽, 刘丽霞. 奶牛CSF3基因遗传多态性筛查及其生物信息学分析[J]. 浙江农业学报, 2020, 32(6): 986-993.
[6]	白俊艳, 卢军浩, 付学言, 武晓红, 杨又兵, 雷莹, 庞有志, 卢小宁, 巩慧荣, 胡陆星, 刘红涛, 樊红灯, 曹恒, 时坤鹏, 陈梦柯, 马永康. 蛋用鹌鹑IGF-1R基因的多态性与体尺性状相关性分析[J]. 浙江农业学报, 2020, 32(3): 398-405.
[7]	谭光辉, 覃媛钰, 李杰章, 吴磊, 岳雍, 张依裕. 樱桃谷鸭ATF4基因SNPs筛查及生物信息学分析[J]. 浙江农业学报, 2020, 32(2): 218-225.
[8]	彭帅, 陈朗, 郑天宇, 陆会宁, 张丽, 刘丽霞. 大通牦牛TLR2基因SNP位点筛选及生物信息学分析[J]. 浙江农业学报, 2019, 31(8): 1280-1285.
[9]	陈琳, 周青青, 顾青, 郦萍. 实时定量PCR法快速检测水产品中的副溶血性弧菌[J]. 浙江农业学报, 2019, 31(5): 823-828.
[10]	乔中全, 王晓明, 李永欣, 曾慧杰, 蔡能, 刘思思, 陈艺. 38个紫薇品种亲缘关系的ISSR分析[J]. 浙江农业学报, 2019, 31(4): 565-571.
[11]	杨又兵, 卞军平, 吴燕, 娄然, 李世豪. 猪FTO基因多态性与肉质性状的关联分析[J]. 浙江农业学报, 2019, 31(12): 1971-1978.
[12]	魏庆镇, 王五宏, 胡天华, 胡海娇, 汪精磊, 包崇来. 浙茄类型茄子品种DNA指纹图谱构建[J]. 浙江农业学报, 2019, 31(11): 1863-1870.
[13]	农伟伦, 毕英杰, 李宏旭, 张丽. 甘肃黑猪H-FABP基因克隆、SNPs筛查及生物信息学分析[J]. 浙江农业学报, 2018, 30(3): 378-385.
[14]	贾小平, 张博, 全建章, 王永芳, 董志平, 袁玺垒, 李剑峰. 洛阳、吉林生态区谷子抗倒伏性的全基因组关联分析[J]. 浙江农业学报, 2018, 30(12): 1981-1991.
[15]	任卫合, 任稳稳, 马博妍, 高成宏, 李宏旭, 刘丽霞, 曹忻, 张丽. 大耳白兔MSTN基因SNPs位点筛查及生物信息学分析[J]. 浙江农业学报, 2018, 30(12): 2018-2023.