浙江农业学报 ›› 2019, Vol. 31 ›› Issue (7): 1154-1160.DOI: 10.3969/j.issn.1004-1524.2019.07.16

• 食品科学 • 上一篇    下一篇

大孔树脂纯化桑葚渣中α-淀粉酶抑制剂的工艺优化及其活性成分研究

范铭1, 向露1, 徐文慧1, 曹艳2, 刘哲2, 陆胜民1,2,*   

  1. 1.浙江师范大学 化学与生命科学学院,浙江 金华 321004;
    2.浙江省农业科学院 食品科学研究所,浙江省果蔬保鲜与加工技术研究重点实验室,农业农村部果品采后处理重点实验室,浙江 杭州 310021
  • 收稿日期:2019-03-04 出版日期:2019-07-25 发布日期:2019-08-07
  • 通讯作者: *陆胜民,E-mail: lushengmin@hotmail.com
  • 作者简介:范铭(1993—),男,江苏溧阳人,硕士研究生,研究方向为果品加工。E-mail: fanm1009@outlook.com
  • 基金资助:
    浙江省重点研发计划(2017C02004); (原)农业部农产品加工重点实验室开放课题

Purification of α-amylase inhibitor from mulberry pomace by macroporous resin and analysis into its active components

FAN Ming1, XIANG Lu1, XU Wenhui1, CAO Yan2, LIU Zhe2, LU Shengmin1,2,*   

  1. 1. College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China;
    2. Key Laboratory of Fruit and Vegetables Postharvest and Processing Technology of Zhejiang Province, Key Laboratory of Post-Harvest Handling of Fruit, Ministry of Agriculture and Rural Affairs, Institute of Food Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
  • Received:2019-03-04 Online:2019-07-25 Published:2019-08-07

摘要: 以桑葚渣为原料,研究了桑葚渣水提液中α-淀粉酶抑制剂的分离纯化方法及其成分。以α-淀粉酶抑制率为指标,对比了5种树脂(AB-8、S-8、D101、NKA-9和HZ-806)对桑葚渣水提液中α-淀粉酶抑制剂静态吸附和解吸的影响,从而筛选出适于分离α-淀粉酶抑制剂的大孔树脂;以此为基础,对桑葚渣水提液中α-淀粉酶抑制剂的分离、纯化参数,如上样流速、洗脱剂体积分数、洗脱剂流速进行优化;利用高相液相色谱分析不同体积分数(10%、30%、50%、70%、90%)乙醇洗脱液中的成分,以期探明桑葚渣中具有α-淀粉酶抑制活性的成分。结果表明:AB-8大孔树脂适于分离桑葚渣水提液中α-淀粉酶抑制剂,分离纯化最佳工艺为上样流速12 mL·min-1,70%体积分数乙醇溶液作为洗脱剂,洗脱剂流速24 mL·min-1。通过分析不同体积分数乙醇洗脱液中的物质组成及其对α-淀粉酶的抑制活性发现,槲皮苷的质量浓度变化与洗脱液对α-淀粉酶的抑制率呈相似趋势,推测其可能是桑葚渣水提物中α-淀粉酶抑制活性的主要贡献因子。

关键词: 桑葚渣, α-淀粉酶抑制剂, 纯化, 活性成分, 槲皮苷

Abstract: In this research, purification process of α-amylase inhibitors derived from mulberry pomace was optimized, and the effective substances was also studied. The effects of different macroporous resins on the static adsorption and desorption of α-amylase inhibitors were investigated. Furthermore, high performance liquid chromatography (HPLC) was applied to analyze the components of eluents obtained from macroporous resin by deionized water or ethanol solutions with different concentrations (10%, 30%, 50%, 70%, 90%). The results showed that AB-8 macroporous resin was the most optimal resin for separating α-amylase inhibitors. The optimum process parameters for dynamic adsorption and desorption of α-amylase inhibitors from mulberry pomace were as follows: sampling rate of 12 mL·min-1, eluent of 70% ethanol solution, and elution flow rate of 24 mL·min-1. It was found that the content of quercetin in the eluent varied accordingly with the α-amylase inhibitory activity of purified mulberry pomace extracts, thus it was inferred that quercetin might be the main contributor of α-amylase inhibitory activity of mulberry pomace extracts.

Key words: mulberry pomace, α-amylase inhibitors, purification, active ingredients, quercetin

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