四种鱚属鱼类线粒体Cyt b基因的序列变异及系统发育研究

doi:10.3969/j.issn.1004-1524.2020.01.05

浙江农业学报 ›› 2020, Vol. 32 ›› Issue (1): 35-42.DOI: 10.3969/j.issn.1004-1524.2020.01.05

四种鱚属鱼类线粒体Cyt b基因的序列变异及系统发育研究

袁冬皓¹, 杨天燕^1,*, 孟玮², 郑瑶¹, 郑德育¹

1. 浙江海洋大学水产学院,浙江舟山 316022;
2. 浙江省海洋水产研究所, 浙江省海洋渔业资源可持续利用技术研究重点实验室, 农业农村部重点渔场渔业资源科学观测实验站, 浙江舟山316021

收稿日期:2019-06-13 出版日期:2020-01-25 发布日期:2020-03-11
通讯作者: ^*杨天燕,E-mail:hellojelly1130@163.com
作者简介:袁冬皓(1997—),男,浙江嘉兴人,学士,主要从事海洋生物资源与环境研究。E-mail:545892170@qq.com
基金资助:
国家自然科学基金(31572227,41776171); 浙江省重点研发计划(2019C02056); 2018年度国家级大学生创新创业训练计划(201810340010); 浙江省大学生科技创新活动计划暨新苗人才计划(2018R411057); 浙江海洋大学2016—2017年度人才引进科研基金; 浙江海洋大学水产省一流学科大学生创新性科研项目

Sequence variation and molecular phylogeny of mitochondrial Cyt b gene segments from four Sillago species

YUAN Donghao¹, YANG Tianyan^1,*, MENG Wei², ZHENG Yao¹, ZHENG Deyu¹

1. Fishery College, Zhejiang Ocean University, Zhoushan 316022, China;
2. Key Laboratory of Sustainable Utilization of Technology Research for Fisheries Resources of Zhejiang Province, Scientific Observing and Experimental Station of Fishery Resources for Key Fishing Grounds, Ministry of Agriculture and Rural Affairs, Marine Fishery Research Institute of Zhejiang Province, Zhoushan 316021, China

Received:2019-06-13 Online:2020-01-25 Published:2020-03-11

摘要/Abstract

摘要： 测定和分析了4种鱚属(Sillago)鱼类:斑鱚(Sillago aeolus)、亚洲鱚(S. asiatica)、多鳞鱚(S. sihama)和少鳞鱚(S. japonica)线粒体细胞色素b基因序列片段,比较了不同鱚属鱼类种间的序列差异,探讨了彼此间系统发育关系和分类地位。结果显示,4种鱼类平均核苷酸组成为T 29.9%、C 29.2%、A 21.5%、G 19.3%,种间平均净遗传距离在0.157到0.280之间。最大似然法构建的系统树显示,少鳞鱚和亚洲鱚首先聚类、再与多鳞鱚和斑鱚相聚,斑鱚是最晚分化出的鱼类。基于Cyt b基因序列核苷酸分歧速率计算得出4种鱼类发生遗传分化时间在距今785—1400年前的第三纪中新世(Miocene)。

关键词: 鱚属, Cyt b, 序列变异, 系统发育, 分化时间

Abstract: Mitochondrial DNA cytochrome b gene sequence fragments of four Sillago species (Sillago aeolus, S. asiatica, S. sihama and S. japonica) were sequenced and analyzed to compare the sequence differences, phylogenetic relationships and taxonomic status among them. The results showed that the average nucleotide composition of the four species was T 29.9%, C 29.2%, A 21.5% and G 19.3%, and the average interspecific genetic distance was between 0.157 and 0.280. The maximum likelihood (ML) phylogenetic tree showed that S. japonica and S. asiatica clustered together first, and then clustered with S. sihama and S. aeolus, respectively. S.aeolus was the latest to divergent from other Sillago species. The genetic divergence time of four Sillago fishes based on the nucleotide divergence rate of Cyt b gene sequence was 785-1400 years ago in the Miocene period.

Key words: Sillago, mitochondrial cytochrome b gene, sequence variance, phylogenetics, divergence time

中图分类号:

S917

袁冬皓, 杨天燕, 孟玮, 郑瑶, 郑德育. 四种鱚属鱼类线粒体Cyt b基因的序列变异及系统发育研究[J]. 浙江农业学报, 2020, 32(1): 35-42.

YUAN Donghao, YANG Tianyan, MENG Wei, ZHENG Yao, ZHENG Deyu. Sequence variation and molecular phylogeny of mitochondrial Cyt b gene segments from four Sillago species[J]. , 2020, 32(1): 35-42.

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四种鱚属鱼类线粒体Cyt b基因的序列变异及系统发育研究

Sequence variation and molecular phylogeny of mitochondrial Cyt b gene segments from four Sillago species

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