浙江省亚洲柑橘木虱黄龙病病原分布与消长规律

doi:10.3969/j.issn.1004-1524.2021.03.11

浙江农业学报 ›› 2021, Vol. 33 ›› Issue (3): 464-469.DOI: 10.3969/j.issn.1004-1524.2021.03.11

浙江省亚洲柑橘木虱黄龙病病原分布与消长规律

孟幼青¹, 汪恩国², 陈吴健³, 李艳敏¹, 程帆³, 孟敏霞⁴

1. 浙江省植保检疫与农药管理总站,浙江杭州 310020
2.临海市农业技术推广中心,浙江临海 317000
3.浙江省检验检疫科学技术研究院,浙江杭州 310016
4.诸暨市农业农村局,浙江诸暨 311800

收稿日期:2020-07-21 出版日期:2021-04-02 发布日期:2021-03-25
作者简介:孟幼青(1963—),女,浙江诸暨人,学士,高级农艺师,主要从事柑橘检疫性有害生物研究。E-mail: yqmeng_77@163.com
基金资助:
浙江省重点研发计划(2015C02007);农业部公益性行业(农业)科研专项(201003067);浙江省科技计划(2004C32087)

Distribution and growth dynamics of Candidatus Liberibacter asiaticus positive Asian citrus psyllid in Zhejiang Province

MENG Youqing¹, WANG Enguo², CHEN Wujian³, LI Yanmin¹, CHENG Fan³, MENG Minxia⁴

1. Zhejiang Plant Protection and Quarantine and Pesticide Management Station, Hangzhou 310020, China
2. Agro-Tech Extension Center of Linhai City, Linhai 317000, China
3. Zhejiang Academy of Science & Technology for Inspection & Quarantine, Hangzhou 310016, China;
4. Bureau of Agriculture and Rural Affairs of Zhuji City, Zhuji 311800, China

Received:2020-07-21 Online:2021-04-02 Published:2021-03-25

摘要/Abstract

摘要：

亚洲柑橘木虱Diaphorina citri Kuwayama 是黄龙病病原Candidatus Liberibacter asiaticus (CLas)的主要传播媒介。为更好地探清亚洲柑橘木虱上的CLas阳性分布特性及其动态变化,提出适宜治虫防病的窗口期,特针对浙江省柑橘果园的分布特点,于2002—2019年采取定园定期和不定园不定期相结合的方式,采检24个县(市、区)重点柑橘果园的亚洲柑橘木虱样品1 662份、黄龙病样品2 900份,通过荧光定量PCR技术测定亚洲柑橘木虱的CLas携菌量及其阳性率。结果显示,浙江省亚洲柑橘木虱的CLas携菌量与所处经纬度分别呈线性关系,根据拟合的线性方程推算,浙江省亚洲柑橘木虱的CLas阳性分布范围为29.20°N以南和119.80°E以东的区域。亚洲柑橘木虱的CLas阳性率随黄龙病发病率增加而上升,两者呈逻辑斯蒂模型(Logistic model)变化规律。亚洲柑橘木虱的CLas阳性率在月际间表现为山峰型波浪式变化结构,峰期分别为4、7、12月;在2001—2019年间的年际变化呈二次曲线,经推算,在2010年达到峰值,然后趋于缓解。据此认为,春、夏、秋3梢初期为最佳窗口期,建议加强3梢初期的治虫防病。

关键词: 亚洲柑橘木虱, 分布特性, 消长规律, 浙江省

Abstract:

Asian citrus psyllid (Diaphorina citri Kuwayama) is the main vector of Candidatus Liberibacter asiaticus(CLas), the pathogen of Huanglongbing (HLB) in China. In order to reveal the distribution characteristics and growth dynamics of CLas in Asian citrus psyllid, and propose suitable time for pest control and disease prevention, 1 662 samples of Asian citrus psyllid and 2 900 samples of HLB were collected from orchards in 24 counties (cities and districts) from 2002 to 2019, according to the distribution characteristics of citrus orchards in Zhejiang Province. The quantity and positive rate of CLas were detected by quantitative PCR. It was shown that there was linear relationship within CLas amount and latitude, longitude, respectively. It was calculated that the north and west boundary for CLas distribution was 29.20°N and 119.80°E in Zhejiang Province. The positive rate of CLas in Zhejiang orchards increased with the elevated HLB incidence rate, and their relationship fitted the Logistic model. The monthly dynamics of CLas in Zhejiang orchards exhibited 3 peaks in April, July and December, respectively, while the annual dynamics fitted conic curves, and reached the peak in 2010. Based on these findings, it was suggested that early spring, summer and autumn were the best time for HLB prevention and control.

Key words: Asian citrus psyllid, distribution characteristics, growth dynamic, Zhejiang Province

中图分类号:

S436.66

孟幼青, 汪恩国, 陈吴健, 李艳敏, 程帆, 孟敏霞. 浙江省亚洲柑橘木虱黄龙病病原分布与消长规律[J]. 浙江农业学报, 2021, 33(3): 464-469.

MENG Youqing, WANG Enguo, CHEN Wujian, LI Yanmin, CHENG Fan, MENG Minxia. Distribution and growth dynamics of Candidatus Liberibacter asiaticus positive Asian citrus psyllid in Zhejiang Province[J]. Acta Agriculturae Zhejiangensis, 2021, 33(3): 464-469.

图/表 5

参考文献 16

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果园地点 Orchard location	调查株数 Plant number	HLB病株率 Percentage of HLB infected plants/%	采集样品虫数 Number of Asian citrus psyllid samples	亚洲柑橘木虱检测虫数 Number of Asian citrus psyllids for detection	亚洲柑橘木虱CLas阳性率 Positive rate of CLas in Asian citrus psyllid/%
黄岩北洋Beiyang, Huangyan	250	0	50	30	6.7
黄岩院桥Yuanqiao, Huangyan	250	0	50	30	7.0
黄岩北洋Beiyang, Huangyan	250	0	50	30	3.3
黄岩澄江Chengjiang, Huangyan	250	2.4	50	30	30.0
黄岩院桥Yuanqiao, Huangyan	270	7.0	50	36	13.3
黄岩院桥Yuanqiao, Huangyan	270	7.0	50	36	13.3
黄岩院桥Yuanqiao, Huangyan	270	12.9	50	30	13.3
黄岩院桥Yuanqiao, Huangyan	270	12.9	50	30	13.3
黄岩院桥Yuanqiao, Huangyan	270	13.1	50	30	10.0
黄岩院桥Yuanqiao, Huangyan	270	13.1	50	30	10.0
乐清湖雾Huwu, Yueqing	270	49.2	50	30	16.7
莲都高溪Gaoxi, Liandu	270	64.8	50	36	36.7
乐清湖雾Huwu, Yueqing	270	98.5	50	30	66.7

果园地点 Orchard location	调查株数 Plant number	HLB病株率 Percentage of HLB infected plants/%	采集样品虫数 Number of Asian citrus psyllid samples	亚洲柑橘木虱检测虫数 Number of Asian citrus psyllids for detection	亚洲柑橘木虱CLas阳性率 Positive rate of CLas in Asian citrus psyllid/%
黄岩北洋Beiyang, Huangyan	250	0	50	30	6.7
黄岩院桥Yuanqiao, Huangyan	250	0	50	30	7.0
黄岩北洋Beiyang, Huangyan	250	0	50	30	3.3
黄岩澄江Chengjiang, Huangyan	250	2.4	50	30	30.0
黄岩院桥Yuanqiao, Huangyan	270	7.0	50	36	13.3
黄岩院桥Yuanqiao, Huangyan	270	7.0	50	36	13.3
黄岩院桥Yuanqiao, Huangyan	270	12.9	50	30	13.3
黄岩院桥Yuanqiao, Huangyan	270	12.9	50	30	13.3
黄岩院桥Yuanqiao, Huangyan	270	13.1	50	30	10.0
黄岩院桥Yuanqiao, Huangyan	270	13.1	50	30	10.0
乐清湖雾Huwu, Yueqing	270	49.2	50	30	16.7
莲都高溪Gaoxi, Liandu	270	64.8	50	36	36.7
乐清湖雾Huwu, Yueqing	270	98.5	50	30	66.7

浙江省亚洲柑橘木虱黄龙病病原分布与消长规律

Distribution and growth dynamics of Candidatus Liberibacter asiaticus positive Asian citrus psyllid in Zhejiang Province

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