干旱胁迫对黄薇光合特性和若干生理生化指标的影响

doi:10.3969/j.issn.1004-1524.2021.09.09

浙江农业学报 ›› 2021, Vol. 33 ›› Issue (9): 1650-1659.DOI: 10.3969/j.issn.1004-1524.2021.09.09

干旱胁迫对黄薇光合特性和若干生理生化指标的影响

郑钢^a^,^b^,^c(), 顾翠花^a^,^b^,^c, 王杰^a^,^b^,^c, 林琳^a^,^b^,^c^,^*()

浙江农林大学 a.风景园林与建筑学院
b.浙江省园林植物种质创新与利用重点实验室
c.南方园林植物种质创新与利用国家林业和草原局重点实验室,浙江杭州 311300

收稿日期:2020-11-13 出版日期:2021-09-25 发布日期:2021-10-09
作者简介:* 林琳,E-mail: 928299135@qq.com
郑钢(1964—),男,浙江诸暨人,学士,实验师,主要从事园林植物遗传育种与种质创新研究。E-mail: 305788868@qq.com
通讯作者: 林琳
基金资助:
浙江省自然科学基金(LY21C160001);浙江省大学生科技活动计划暨新苗人才计划(2021R412051)

Effects of drought stress on photosynthetic characteristics and several physiological and biochemical indexes of Heimia myrtifolia Cham.et Schlechtend.

ZHENG Gang^a^,^b^,^c(), GU Cuihua^a^,^b^,^c, WANG Jie^a^,^b^,^c, LIN Lin^a^,^b^,^c^,^*()

a. School of Landscape and Architecture
b. Zhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants
c. Key Laboratory of National Forestry and Grassland Administration on Germplasm Innovation and Utilization for Southern Garden Plants, Zhejiang A & F University, Hangzhou 311300, China

Received:2020-11-13 Online:2021-09-25 Published:2021-10-09
Contact: LIN Lin

摘要/Abstract

摘要：

为探究黄薇(Heimia myrtifolia)对干旱胁迫的适应能力,以黄薇一年生扦插苗为试验材料,设置5个水分处理(CK、T1、T2、T3、T4,土壤相对含水量分别保持在65%~75%、45%~60%、30%~45%、15%~30%、5%~15%),采用盆栽试验连续处理20 d,研究黄薇部分生理生化指标、光合作用和气孔变化对干旱胁迫的响应。结果表明:随着干旱程度的加深,黄薇叶片的净光合速率、蒸腾速率、气孔导度显著(P<0.05)下降,胞间二氧化碳浓度先降后升,类胡萝卜素、叶绿素和叶绿素a、b含量呈上升趋势,叶片下表皮气孔随着土壤水分散失而关闭,气孔形态结构也发生适应性的变化;丙二醛含量持续增高,过氧化氢酶活性和过氧化物酶活性先上升后下降,超氧化物歧化酶在T1~T2处理下保持较高活性,在T3处理下降至CK水平;脯氨酸含量先下降后上升,可溶性糖含量显著(P<0.05)增加。综上,黄薇对轻中度干旱具有良好的适应性,但不能承受长时间的重度干旱。研究结果可为后续黄薇的引种驯化和培育应用提供理论依据。

关键词: 黄薇, 干旱胁迫, 抗氧化酶, 渗透调节物质, 光合参数, 气孔

Abstract:

To explore the resistance of Heimia myrtifolia Cham.et Schlechtend. to drought stress, the one-year cutting seedlings of Heimia myrtifolia were used as the experimental materials with five treatments (namely, CK, T1, T2, T3, T4, and the soil water content was controled at 65%-75%, 45%-60%, 30%-45%, 15%-30%, 5%-15%, accordingly) for 20 d, to study responses of several physiological and biochemical indexes, and photosynthesis and stomatal characteristics of Heimia myrtifolia under drought stress. The results showed that the net photosynthetic rate, transpiration rate and stomatal conductance decreased significantly (P<0.05) with the elevated drought stress, while the intercellular carbon dioxide concentration decreased firstly then increased. The contents of carotenoids, chlorophyll and chlorophyll a, b all showed an upward trend with the elevated drought stress, while the inferior epidermal porosity of leaves closed with the loss of soil water, and the stomatal morphology and structure also changed adaptively. Malondialdehyde content kept on increasing, while the catalase activity and peroxidase activity first increased and then decreased with the elevated drought stress. The superoxide dismutase activity remained relatively active under T1 and T2 treatments, yet went down to CK level under T3 treatment. Proline content decreased first and then increased, while soluble sugar content increased significantly (P<0.05) with the elevated drought stress. To sum up, Heimia myrtifolia could resistant to mild and moderate drought, but it could not withstand prolonged, severe drought. The results could provide theoretical basis for the introduction, domestication and cultivation of Heimia myrtifolia in the future.

Key words: Heimia myrtifolia, drought stress, antioxidant enzymes, osmotic regulation substances, photosynthetic parameters, stomata

中图分类号:

S793.9

郑钢, 顾翠花, 王杰, 林琳. 干旱胁迫对黄薇光合特性和若干生理生化指标的影响[J]. 浙江农业学报, 2021, 33(9): 1650-1659.

ZHENG Gang, GU Cuihua, WANG Jie, LIN Lin. Effects of drought stress on photosynthetic characteristics and several physiological and biochemical indexes of Heimia myrtifolia Cham.et Schlechtend.[J]. Acta Agriculturae Zhejiangensis, 2021, 33(9): 1650-1659.

图/表 6

图1 不同处理下黄薇叶片的丙二醛含量和抗氧化酶活性变化柱上无相同字母的表示处理间差异显著(P<0. 05)。下同。

Fig.1 Changes of malondialdehyde content and antioxidant enzymes activities in leaves of Heimia myrtifolia under different treatments Bars marked without the same letters indicated significant difference at P<0.05. The same as below. MDA, Malondialdehyde; CAT, Catalase; SOD, Superoxide dismutase; POD, Peroxidase.The same as below.

图2 不同处理下黄薇叶片的渗透调节物质含量变化

Fig.2 Changes of osmotic regulation substances in leaves of Heimia myrtifolia under different treatments SS, Soluble sugar.

图3 不同处理下黄薇叶片的光合参数变化 Pn,净光合速率;Tr,蒸腾速率;Ci,胞间CO2深度;Gs,气孔导度。

Fig.3 Changes of photosynthetic parameters in leaves of Heimia myrtifolia under different treatments Pn, Net photosynthetic rate; Tr, Transpiration rate; Ci, Intercellular carbon dioxide; Gs, Stomatal conductance.

图4 不同处理下黄薇叶片的光合色素含量变化

Fig.4 Contents of photosynthetic pigments in leaves of Heimia myrtifolia under different treatments

表1 不同处理下黄薇叶片的气孔长、宽、面积、密度变化

Table 1 Stomatal length, width, area and density in leaves of Heimia myrtifolia under different treatments

处理Treatment	SL/μm	SW/μm	SA/μm²	SD/μm^-2
CK	30.8±1.8 a	20.6±1.7 a	487.1±53.6 a	177.6±8.7 a
T1	29.1±1.3 b	19.4±1.4 b	435.1±32.0 b	164.3±5.4 b
T2	25.2±1.8 c	16.2±1.2 c	327.3±34.1 c	162.7±4.0 b
T3	23.1±1.1 d	15.5±1.2 d	297.4±19.6 d	149.8±4.4 c
T4	22.9±1.3 e	16.0±1.4 cd	285.9±24.7 d	143.3±5.5 d

表2 不同处理下黄薇叶片各生理生化指标的相关性

Table 2 Correlation of physiological and biochemical indexes in leaves of Heimia myrtifolia under different treatments

指标 Index	MDA	POD	CAT	SOD	Pro	SS	Chl	P_n	C_i	G_s	T_r	SA
POD	-0.524^**
CAT	-0.483^*	0.877^**
SOD	-0.875^**	0.676^**	0.730^*
Pro	0.782^**	-0.838^**	-0.679^**	-0.818^**
SS	0.882^**	-0.255	-0.077	-0.642^**	0.653^**
Chl	0.904^**	-0.409^*	-0.221	-0.661^**	0.729^**	0.943^**
P_n	-0.945^**	0.348	0.242	0.767^**	-0.711^**	-0.974^**	-0.926^**
C_i	-0.271	-0.579^**	-0.672^**	-0.066	0.129	-0.589^**	-0.462^*	0.493^*
G_s	-0.889^**	0.202	0.067	0.607^**	-0.583^**	-0.973^**	-0.942^**	0.963^**	0.631^**
T_r	-0.740^**	-0.146	-0.177	0.418^*	-0.265	-0.861^*	-0.786^**	0.842^**	0.808^**	0.925^*
SA	-0.856^**	0.222	0.034	0.612^**	-0.617^**	-0.984^**	-0.916^**	0.953^**	0.611^**	0.964^**	0.865^**
SD	-0.875^**	0.202	0.140	0.631^**	-0.533^**	-0.905^**	-0.906^**	0.913^**	0.555^**	0.946^**	0.891^**	0.897^**

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	WU Y B, YE B. Effects of combined elevated temperature and drought stress on anti-oxidative enzyme activities and reactive oxygen species metabolism of Broussonetia papyrifera seedlings[J]. Acta Ecologica Sinica, 2016, 36(2):403-410.(in Chinese with English abstract)

干旱胁迫对黄薇光合特性和若干生理生化指标的影响

Effects of drought stress on photosynthetic characteristics and several physiological and biochemical indexes of Heimia myrtifolia Cham.et Schlechtend.

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