磺胺间甲氧嘧啶对蛋鸡粪便菌群结构的影响

doi:10.3969/j.issn.1004-1524.2022.02.09

浙江农业学报 ›› 2022, Vol. 34 ›› Issue (2): 284-292.DOI: 10.3969/j.issn.1004-1524.2022.02.09

磺胺间甲氧嘧啶对蛋鸡粪便菌群结构的影响

王静鸽¹^,²^,³(), 吉小凤²^,³, 吴静²^,³, 杨华²^,³, 唐标²^,³^,^*(), 丁保安¹^,^*()

1.青海大学农牧学院,青海西宁 810016
2.浙江省农业科学院农产品质量安全与营养研究所,浙江杭州 310021
3.浙江省农业科学院农产品质量安全危害因子与风险防控国家重点实验室,浙江杭州 310021

收稿日期:2021-03-15 出版日期:2022-02-25 发布日期:2022-03-02
通讯作者: 唐标,丁保安
作者简介:唐标,E-mail: tb_411@163.com
丁保安,E-mail: dingbaoan1967@163.com;
王静鸽(1994—),女,河南洛阳人,硕士研究生,研究方向为动物营养与饲料科学。E-mail: wangjingge2021@163.com
基金资助:
浙江省重点研发计划(2020C02031);国家自然科学基金(31700007);省部共建农产品质量安全危害因子与风险防控国家重点实验室项目(2010DS700124-ZZ2008);浙江省农业重大技术协同推广计划(2021XTTGXM03-3)

Effects of sulfamonomethoxine on bacterial community structure in feces of laying hens

WANG Jingge¹^,²^,³(), JI Xiaofeng²^,³, WU Jing²^,³, YANG Hua²^,³, TANG Biao²^,³^,^*(), DING Bao'an¹^,^*()

1. College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China
2. Institute of Agro-Product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
3. State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2021-03-15 Online:2022-02-25 Published:2022-03-02
Contact: TANG Biao,DING Bao'an

摘要/Abstract

摘要：

为研究蛋鸡养殖过程中,向饮用水中添加磺胺间甲氧嘧啶(SMM)对蛋鸡粪便菌群结构的影响,选取海兰褐蛋鸡260只,随机平均分为两组(A1组,饮用水中不添加SMM;B1组,饮用水中添加SMM),饲养5 d后采集新鲜粪便,之后B1组停止添加SMM作为B2组,A1组重新编号为A2组,作为B2组的对照组,继续饲养7 d后采集新鲜粪便样品。样品抽提总DNA后,利用Illumina MiSeq高通量测序技术,基于16S rDNA的V3~V4可变区进行多样性分析和粪便细菌群落结构特征分析。结果显示,SMM的使用显著降低了蛋鸡粪便菌群的多样性和丰富度,但乳杆菌属(Lactobacillus)和不动杆菌属(Acinetobacter)的丰度显著(P<0.05)增加。停止使用SMM一周后,蛋鸡粪便菌群的多样性和丰富度得到恢复,不动杆菌属(Acinetobacter)的丰度显著(P<0.05)下降。综上,饲喂SMM改变了蛋鸡肠道的菌群结构,停药后,蛋鸡肠道的菌群可得到恢复。研究结果可为明确抗生素的危害、规范SMM使用等提供参考。

关键词: 磺胺间甲氧嘧啶, 蛋鸡, 细菌群落结构

Abstract:

In order to explore the effects of sulfamonomethoxine (SMM) addition into the drinking water during breeding on the structure of fecal bacterial community of laying hens, a total of 260 Hailan brown laying hens were randomly divided into two groups (group A1: no SMM was added into drinking water; group B1: SMM was added into drinking water), and fresh feces were collected on the 5th day of feeding. Then, no SMM was added in group B1, and this group was renamed as group B2, and group A1 was renamed as A2 (as the control of group B2), and fresh feces were collected again after 7 days of feeding. The total DNA was extracted and sequenced by Illumina MiSeq platform. Based on the variable region of V3-V4 of 16S rDNA, diversity and fecal microbial community structure were analyzed. The addition of SMM reduced the bacterial diversity and richness of the laying hens, but the abundance of Lactobacillus and Acinetobacter were significantly (P<0.05) increased. One week after stopping SMM addtiion, the bacterial diversity and richness of the laying hens recovered, and the abundance of Acinetobacter was significantly (P<0.05) decreased. It was concluded that feeding SMM could change the structure of the intestinal community of laying hens, yet the intestinal community could recover after discontinuation of SMM feeding. The results increased insight into the harm of antibiotics and provided references for the standard use of antibiotics.

Key words: sulfamonomethoxine, laying hen, bacterial community structure

中图分类号:

S811.6

王静鸽, 吉小凤, 吴静, 杨华, 唐标, 丁保安. 磺胺间甲氧嘧啶对蛋鸡粪便菌群结构的影响[J]. 浙江农业学报, 2022, 34(2): 284-292.

WANG Jingge, JI Xiaofeng, WU Jing, YANG Hua, TANG Biao, DING Bao'an. Effects of sulfamonomethoxine on bacterial community structure in feces of laying hens[J]. Acta Agriculturae Zhejiangensis, 2022, 34(2): 284-292.

图/表 6

图1 样品OTU韦恩图

Fig.1 Venn diagram of sample OTUs

表1 多样性分析统计

Table 1 Diversity statistics

样品 Sample	Coverage指数 Coverage index	Ace指数 Ace index	Simpson指数 Simpson index	Sobs指数 Sobs index
A1	0.996	683.674	0.092	569.727
B1	0.997	610.480	0.133	442.667
A2	0.995	746.428	0.080	615.636
B2	0.995	745.682	0.113	589.533

图2 不同样品在门水平上的菌群组成Circos图

Fig.2 Circos diagram of bacterial communities composition of different samples at phylum level

图3 不同样品在属水平上的菌群组成热图

Fig.3 Heat map of bacterial communities composition of different samples at genus level

图4 样品间属水平上的菌群差异分析

Fig.4 Differences within groups at genus level

图5 A1组与B1组、B1组与B2组样品菌群属水平上的差异圆点对应的数值表示物种在两组中平均相对丰度的差值,圆点颜色显示为物种丰度占比较大的分组颜色,圆点上的I型区间为差值的上下限值,最右边为P值。*,0.01<P≤0.05;**,0.001<P≤0.01;***,P≤0.001。

Fig.5 Differences within groups at genus level between group A1 and B1, group B1 and B2 The value corresponding to the dot represents the difference in the average relative abundance of species in the two groups. The color of the dot shows the grouping color with a relatively larger species abundance, and the type I interval on the dot is the upper and lower limit of the difference. The P value is on the right. *, 0.01<P≤0.05; **, 0.001<P≤0.01; ***, P≤0.001.

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磺胺间甲氧嘧啶对蛋鸡粪便菌群结构的影响

Effects of sulfamonomethoxine on bacterial community structure in feces of laying hens

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