浙江农业学报 ›› 2022, Vol. 34 ›› Issue (6): 1175-1182.DOI: 10.3969/j.issn.1004-1524.2022.06.08
收稿日期:
2021-09-14
出版日期:
2022-06-25
发布日期:
2022-06-30
通讯作者:
马春晖
作者简介:
*马春晖,E-mail: machunhui2000@163.com基金资助:
LI Hanfena(), LI Dinglib, WANG Ranb, MA Chunhuib,*(
)
Received:
2021-09-14
Online:
2022-06-25
Published:
2022-06-30
Contact:
MA Chunhui
摘要:
为探索不同梨矮化中间砧对树体生长发育的影响,本试验以杜梨为基砧,嫁接23种不同中间砧与黄金梨砧穗组合为试材,于第4个生长季调查了嫁接口周长、中间砧周长、接穗新梢长度、新梢叶片数、以及叶片卷曲度等指标,统计分析结果显示:BA29、OHF97、K6矮化中间砧对黄金梨的致矮效果最好;中间砧嫁接亲和性与砧木周长、枝梢及叶片生长存在一定的相关性,相关性顺序依次为基砧周长>新梢节间长度>上接口周长与接穗周长的比值(D/E)>下接口周长与中间砧周长比值(B/C)>新梢叶片卷曲度>新梢长度,在亲和性早期鉴定中可用新梢叶片卷曲度来判断亲和力的大小,以上接口与接穗周长的比值(D/E)来表示亲和力更准确。
中图分类号:
李含芬, 李鼎立, 王然, 马春晖. 不同矮化中间砧对黄金梨树体生长的影响及嫁接亲和性相关性分析[J]. 浙江农业学报, 2022, 34(6): 1175-1182.
LI Hanfen, LI Dingli, WANG Ran, MA Chunhui. Effects of different dwarfing interstocks on Whangkumbae pear growth and correlation analysis of graft-compatibility[J]. Acta Agriculturae Zhejiangensis, 2022, 34(6): 1175-1182.
中间砧 Interstocks | 株高 Plant height/cm | 新梢 Shoots | |||
---|---|---|---|---|---|
长度 Length/cm | 节间长 Internode length/cm | 叶数 Leaf number | 叶卷曲度 Leaf curl | ||
E6-3 | 247.7±6.7 | 47.9±5.8 | 4.7±0.7 | 15.0±1.5 | 3.7±0.4 |
E6-5 | 264.9±3.2 | 40.2±3.0 | 5.3±0.5 | 14.0±2.0 | 3.7±0.4 |
E6-7 | 279.0±16.7 | 38.3±6.6 | 5.1±0.4 | 12.7±0.8 | 3.7±0.4 |
E6-16 | 243.9±5.9 | 54.0±1.5 | 4.5±0.3 | 14.5±2.1 | 4.0±0.1 |
E6-22 | 254.0±21.2 | 57.0±4.5 | 4.8±0.4 | 15.5±1.1 | 3.5±0.3 |
E6-42 | 259.1±4.6 | 45.6±3.8 | 4.7±0.2 | 14.7±1.1 | 3.0±0.7 |
E6-50 | 222.2±26.3 | 53.8±4.4 | 4.3±0.5 | 20.7±0.8 | 2.0±0.7 |
K1 | 230.4±11.2 | 41.4±5.9 | 4.2±0.2 | 13.5±1.1 | 3.5±0.3 |
K2 | 219.2±18.1 | 48.2±3.4 | 4.2±0.5 | 15.0±2.0 | 3.0±0.1 |
K3 | 273.2±14.0 | 40.3±0.5 | 4.5±0.3 | 14.5±1.1 | 3.0±0.1 |
K4 | 233.6±3.5 | 41.1±5.5 | 3.7±0.5 | 14.3±1.5 | 3.0±0.1 |
K6 | 201.0±6.8 | 40.8±4.7 | 3.9±0.3 | 13.5±0.3 | 3.0±0.1 |
K7 | 262.8±20.6 | 47.8±7.5 | 4.4±0.4 | 17.0±2.2 | 4.0±0.1 |
K8 | 250.0±7.6 | 46.8±3.2 | 4.6±0.4 | 12.0±0.7 | 3.0±0.7 |
K9 | 282.0±6.1 | 44.8±6.6 | 3.9±0.3 | 13.5±1.1 | 2.5±0.3 |
K10 | 216.0±9.2 | 47.5±2.8 | 4.0±0.4 | 16.0±0.7 | 2.0±0.1 |
K11 | 209.9±20.7 | 47.7±4.1 | 4.4±0.5 | 17.3±2.2 | 2.0±0.1 |
S2 | 225.1±23.0 | 45.8±4.0 | 4.7±0.6 | 14.0±0.8 | 3.3±0.8 |
OHF51 | 232.5±5.8 | 41.9±3.0 | 4.6±0.4 | 12.7±1.2 | 4.0±0.1 |
OHF40 | 204.5±8.2 | 52.5±5.6 | 4.2±0.6 | 13.0±0.7 | 2.3±0.4 |
OHF333 | 201.3±8.8 | 42.1±4.9 | 4.0±1.2 | 13.7±1.9 | 2.5±0.3 |
OHF97 | 201.3±12.9 | 40.1±3.4 | 4.0±0.4 | 15.3±0.7 | 2.7±0.4 |
BA29 | 184.5±14.3 | 43.5±2.5 | 4.2±0.1 | 13.5±3.0 | 2.5±0.1 |
表1 不同中间砧对黄金梨生长发育的影响
Table 1 Effect of different interstocks on growth of Whangkumbae pear
中间砧 Interstocks | 株高 Plant height/cm | 新梢 Shoots | |||
---|---|---|---|---|---|
长度 Length/cm | 节间长 Internode length/cm | 叶数 Leaf number | 叶卷曲度 Leaf curl | ||
E6-3 | 247.7±6.7 | 47.9±5.8 | 4.7±0.7 | 15.0±1.5 | 3.7±0.4 |
E6-5 | 264.9±3.2 | 40.2±3.0 | 5.3±0.5 | 14.0±2.0 | 3.7±0.4 |
E6-7 | 279.0±16.7 | 38.3±6.6 | 5.1±0.4 | 12.7±0.8 | 3.7±0.4 |
E6-16 | 243.9±5.9 | 54.0±1.5 | 4.5±0.3 | 14.5±2.1 | 4.0±0.1 |
E6-22 | 254.0±21.2 | 57.0±4.5 | 4.8±0.4 | 15.5±1.1 | 3.5±0.3 |
E6-42 | 259.1±4.6 | 45.6±3.8 | 4.7±0.2 | 14.7±1.1 | 3.0±0.7 |
E6-50 | 222.2±26.3 | 53.8±4.4 | 4.3±0.5 | 20.7±0.8 | 2.0±0.7 |
K1 | 230.4±11.2 | 41.4±5.9 | 4.2±0.2 | 13.5±1.1 | 3.5±0.3 |
K2 | 219.2±18.1 | 48.2±3.4 | 4.2±0.5 | 15.0±2.0 | 3.0±0.1 |
K3 | 273.2±14.0 | 40.3±0.5 | 4.5±0.3 | 14.5±1.1 | 3.0±0.1 |
K4 | 233.6±3.5 | 41.1±5.5 | 3.7±0.5 | 14.3±1.5 | 3.0±0.1 |
K6 | 201.0±6.8 | 40.8±4.7 | 3.9±0.3 | 13.5±0.3 | 3.0±0.1 |
K7 | 262.8±20.6 | 47.8±7.5 | 4.4±0.4 | 17.0±2.2 | 4.0±0.1 |
K8 | 250.0±7.6 | 46.8±3.2 | 4.6±0.4 | 12.0±0.7 | 3.0±0.7 |
K9 | 282.0±6.1 | 44.8±6.6 | 3.9±0.3 | 13.5±1.1 | 2.5±0.3 |
K10 | 216.0±9.2 | 47.5±2.8 | 4.0±0.4 | 16.0±0.7 | 2.0±0.1 |
K11 | 209.9±20.7 | 47.7±4.1 | 4.4±0.5 | 17.3±2.2 | 2.0±0.1 |
S2 | 225.1±23.0 | 45.8±4.0 | 4.7±0.6 | 14.0±0.8 | 3.3±0.8 |
OHF51 | 232.5±5.8 | 41.9±3.0 | 4.6±0.4 | 12.7±1.2 | 4.0±0.1 |
OHF40 | 204.5±8.2 | 52.5±5.6 | 4.2±0.6 | 13.0±0.7 | 2.3±0.4 |
OHF333 | 201.3±8.8 | 42.1±4.9 | 4.0±1.2 | 13.7±1.9 | 2.5±0.3 |
OHF97 | 201.3±12.9 | 40.1±3.4 | 4.0±0.4 | 15.3±0.7 | 2.7±0.4 |
BA29 | 184.5±14.3 | 43.5±2.5 | 4.2±0.1 | 13.5±3.0 | 2.5±0.1 |
中间砧 Interstocks | 周长Circumference/cm | 嫁接亲和性Graft compatibility | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
基砧 Base stock | 下接口 Lower graft union | 中间砧Interstock | 上接口 Upper graft union | 接穗 Scion | B/C | D/E | A/C | C/E | ||
E6-3 | 10.9±0.8 | 11.1±0.3 | 7.9±0.3 | 13.1±1.1 | 5.8±0.4 | 1.4±0.1 | 2.3±0.1 | 1.4±0.1 | 1.4±0.1 | |
E6-5 | 10.5±0.7 | 10.9±0.6 | 7.9±0.3 | 13.3±0.5 | 5.7±0.2 | 1.4±0.1 | 2.3±0.1 | 1.3±0.1 | 1.4±0.1 | |
E6-7 | 8.4±0.5 | 9.6±0.5 | 7.3±0.6 | 11.4±0.6 | 5.7±0.3 | 1.3±0.2 | 2.0±0.2 | 1.2±0.1 | 1.3±0.1 | |
E6-16 | 9.9±0.3 | 10.9±0.7 | 7.2±0.3 | 12.6±0.5 | 5.6±0.4 | 1.5±0.1 | 2.2±0.3 | 1.4±0.1 | 1.3±0.1 | |
E6-22 | 12.0±1.9 | 10.3±1.6 | 6.3±0.7 | 12.3±0.3 | 5.7±0.6 | 1.6±0.1 | 2.2±0.1 | 1.9±0.1 | 1.1±0.1 | |
E6-42 | 12.8±0.3 | 13.4±0.2 | 9.2±0.4 | 14.8±0.8 | 6.0±0.1 | 1.5±0.1 | 2.3±0.1 | 1.4±0.1 | 1.5±0.1 | |
E6-50 | 9.1±0.9 | 9.8±1.1 | 7.8±1.2 | 11.4±1.5 | 5.0±0.6 | 1.3±0.1 | 2.3±0.1 | 1.2±0.1 | 1.6±0.1 | |
K1 | 10.1±0.6 | 11.5±0.5 | 8.4±0.5 | 9.7±0.6 | 6.9±0.2 | 1.4±0.2 | 1.4±0.1 | 1.2±0.1 | 1.2±0.1 | |
K2 | 9.8±0.4 | 11.0±0.7 | 5.6±0.5 | 10.1±0.4 | 7.0±0.4 | 1.3±1.2 | 1.4±0.1 | 1.8±1.2 | 0.8±0.3 | |
K3 | 10.2±0.5 | 12.0±0.4 | 8.5±0.3 | 11.2±0.6 | 6.9±0.5 | 1.4±0.0 | 1.6±0.1 | 1.2±0.1 | 1.2±0.1 | |
K4 | 9.5±0.6 | 10.0±1.2 | 8.7±0.2 | 11.2±0.8 | 6.6±0.3 | 1.2±0.1 | 1.7±0.2 | 1.1±0.1 | 1.3±0.1 | |
K6 | 8.4±0.4 | 9.4±0.8 | 7.3±0.6 | 9.2±0.7 | 6.5±0.5 | 1.3±0.2 | 1.4±0.2 | 1.2±0.1 | 1.1±0.1 | |
K7 | 9.7±0.1 | 12.3±0.2 | 9.3±0.2 | 10.3±0.2 | 6.7±0.2 | 1.3±0.0 | 1.5±0.1 | 1.0±0.0 | 1.4±0.1 | |
K8 | 9.1±0.8 | 11.8±0.6 | 8.3±0.2 | 10.3±1.1 | 6.9±0.2 | 1.4±0.1 | 1.5±0.1 | 1.1±0.1 | 1.2±0.1 | |
K9 | 10.3±0.5 | 11.8±0.7 | 8.7±0.3 | 10.3±0.2 | 6.9±0.1 | 1.4±0.1 | 1.5±0.1 | 1.2±0.1 | 1.3±0.1 | |
K10 | 8.1±0.6 | 8.3±0.4 | 7.6±0.9 | 8.8±0.5 | 6.3±0.6 | 1.1±0.2 | 1.4±0.2 | 1.1±0.2 | 1.2±0.1 | |
K11 | 10.1±0.4 | 11.1±0.8 | 8.0±1.2 | 9.5±1.4 | 6.0±0.4 | 1.4±0.3 | 1.6±0.2 | 1.3±0.2 | 1.3±0.1 | |
S2 | 8.2±0.6 | 9.9±0.5 | 8.0±0.7 | 8.8±0.4 | 5.8±0.2 | 1.2±0.1 | 1.5±0.1 | 1.0±0.1 | 1.4±0.1 | |
OHF51 | 9.1±0.4 | 11.2±0.6 | 8.3±0.1 | 9.5±0.4 | 6.2±0.3 | 1.4±0.1 | 1.5±0.0 | 1.1±0.1 | 1.3±0.1 | |
OHF40 | 7.7±0.8 | 13.9±1.1 | 9.2±0.7 | 13.0±0.4 | 5.8±0.2 | 1.5±0.2 | 1.9±0.1 | 0.8±0.1 | 1.6±0.1 | |
OHF333 | 8.7±1.2 | 10.8±0.9 | 8.5±0.3 | 11.5±1.0 | 5.5±0.2 | 1.3±0.1 | 1.8±0.2 | 1.0±0.2 | 1.6±0.1 | |
OHF97 | 8.5±0.4 | 10.5±0.8 | 7.6±1.0 | 7.6±1.1 | 5.3±0.6 | 1.4±0.1 | 1.6±0.1 | 1.1±0.2 | 1.4±0.1 | |
BA29 | 6.7±0.3 | 11.8±0.8 | 8.0±0.5 | 9.6±0.7 | 9.6±0.5 | 1.5±0.2 | 1.0±0.3 | 0.8±0.1 | 0.8±0.1 |
表2 不同梨中间砧与黄金梨嫁接亲和性的差异
Table 2 Effect of different interstock on the graft compatibility of Whangkumbae pear
中间砧 Interstocks | 周长Circumference/cm | 嫁接亲和性Graft compatibility | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
基砧 Base stock | 下接口 Lower graft union | 中间砧Interstock | 上接口 Upper graft union | 接穗 Scion | B/C | D/E | A/C | C/E | ||
E6-3 | 10.9±0.8 | 11.1±0.3 | 7.9±0.3 | 13.1±1.1 | 5.8±0.4 | 1.4±0.1 | 2.3±0.1 | 1.4±0.1 | 1.4±0.1 | |
E6-5 | 10.5±0.7 | 10.9±0.6 | 7.9±0.3 | 13.3±0.5 | 5.7±0.2 | 1.4±0.1 | 2.3±0.1 | 1.3±0.1 | 1.4±0.1 | |
E6-7 | 8.4±0.5 | 9.6±0.5 | 7.3±0.6 | 11.4±0.6 | 5.7±0.3 | 1.3±0.2 | 2.0±0.2 | 1.2±0.1 | 1.3±0.1 | |
E6-16 | 9.9±0.3 | 10.9±0.7 | 7.2±0.3 | 12.6±0.5 | 5.6±0.4 | 1.5±0.1 | 2.2±0.3 | 1.4±0.1 | 1.3±0.1 | |
E6-22 | 12.0±1.9 | 10.3±1.6 | 6.3±0.7 | 12.3±0.3 | 5.7±0.6 | 1.6±0.1 | 2.2±0.1 | 1.9±0.1 | 1.1±0.1 | |
E6-42 | 12.8±0.3 | 13.4±0.2 | 9.2±0.4 | 14.8±0.8 | 6.0±0.1 | 1.5±0.1 | 2.3±0.1 | 1.4±0.1 | 1.5±0.1 | |
E6-50 | 9.1±0.9 | 9.8±1.1 | 7.8±1.2 | 11.4±1.5 | 5.0±0.6 | 1.3±0.1 | 2.3±0.1 | 1.2±0.1 | 1.6±0.1 | |
K1 | 10.1±0.6 | 11.5±0.5 | 8.4±0.5 | 9.7±0.6 | 6.9±0.2 | 1.4±0.2 | 1.4±0.1 | 1.2±0.1 | 1.2±0.1 | |
K2 | 9.8±0.4 | 11.0±0.7 | 5.6±0.5 | 10.1±0.4 | 7.0±0.4 | 1.3±1.2 | 1.4±0.1 | 1.8±1.2 | 0.8±0.3 | |
K3 | 10.2±0.5 | 12.0±0.4 | 8.5±0.3 | 11.2±0.6 | 6.9±0.5 | 1.4±0.0 | 1.6±0.1 | 1.2±0.1 | 1.2±0.1 | |
K4 | 9.5±0.6 | 10.0±1.2 | 8.7±0.2 | 11.2±0.8 | 6.6±0.3 | 1.2±0.1 | 1.7±0.2 | 1.1±0.1 | 1.3±0.1 | |
K6 | 8.4±0.4 | 9.4±0.8 | 7.3±0.6 | 9.2±0.7 | 6.5±0.5 | 1.3±0.2 | 1.4±0.2 | 1.2±0.1 | 1.1±0.1 | |
K7 | 9.7±0.1 | 12.3±0.2 | 9.3±0.2 | 10.3±0.2 | 6.7±0.2 | 1.3±0.0 | 1.5±0.1 | 1.0±0.0 | 1.4±0.1 | |
K8 | 9.1±0.8 | 11.8±0.6 | 8.3±0.2 | 10.3±1.1 | 6.9±0.2 | 1.4±0.1 | 1.5±0.1 | 1.1±0.1 | 1.2±0.1 | |
K9 | 10.3±0.5 | 11.8±0.7 | 8.7±0.3 | 10.3±0.2 | 6.9±0.1 | 1.4±0.1 | 1.5±0.1 | 1.2±0.1 | 1.3±0.1 | |
K10 | 8.1±0.6 | 8.3±0.4 | 7.6±0.9 | 8.8±0.5 | 6.3±0.6 | 1.1±0.2 | 1.4±0.2 | 1.1±0.2 | 1.2±0.1 | |
K11 | 10.1±0.4 | 11.1±0.8 | 8.0±1.2 | 9.5±1.4 | 6.0±0.4 | 1.4±0.3 | 1.6±0.2 | 1.3±0.2 | 1.3±0.1 | |
S2 | 8.2±0.6 | 9.9±0.5 | 8.0±0.7 | 8.8±0.4 | 5.8±0.2 | 1.2±0.1 | 1.5±0.1 | 1.0±0.1 | 1.4±0.1 | |
OHF51 | 9.1±0.4 | 11.2±0.6 | 8.3±0.1 | 9.5±0.4 | 6.2±0.3 | 1.4±0.1 | 1.5±0.0 | 1.1±0.1 | 1.3±0.1 | |
OHF40 | 7.7±0.8 | 13.9±1.1 | 9.2±0.7 | 13.0±0.4 | 5.8±0.2 | 1.5±0.2 | 1.9±0.1 | 0.8±0.1 | 1.6±0.1 | |
OHF333 | 8.7±1.2 | 10.8±0.9 | 8.5±0.3 | 11.5±1.0 | 5.5±0.2 | 1.3±0.1 | 1.8±0.2 | 1.0±0.2 | 1.6±0.1 | |
OHF97 | 8.5±0.4 | 10.5±0.8 | 7.6±1.0 | 7.6±1.1 | 5.3±0.6 | 1.4±0.1 | 1.6±0.1 | 1.1±0.2 | 1.4±0.1 | |
BA29 | 6.7±0.3 | 11.8±0.8 | 8.0±0.5 | 9.6±0.7 | 9.6±0.5 | 1.5±0.2 | 1.0±0.3 | 0.8±0.1 | 0.8±0.1 |
图5 不同嫁接亲和性指标参数主成分分析 A,基砧周长;B,下接口周长;C,中间砧周长;D,上接口周长;E,接穗周长;AC,A/C; BC,B/C; DE,D/E; Height,株高;Internode,节间长;Curl,叶片卷曲度;Leaf,叶片数;Shoot,新梢。
Fig. 5 Principal components analysis of graft compatibility index A,Perimeter of base anvil; B, Perimeter of lower interface; C, Perimeter of middle anvil; D, Perimeter of upper interface; E, Scion perimeter.
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