亚致死剂量氰戊菊酯对家蚕生长发育的影响

doi:10.3969/j.issn.1004-1524.20230268

浙江农业学报 ›› 2024, Vol. 36 ›› Issue (2): 264-271.DOI: 10.3969/j.issn.1004-1524.20230268

亚致死剂量氰戊菊酯对家蚕生长发育的影响

俞瑞鲜¹^,²(), 胡秀卿¹^,², 柳新菊¹^,², 汤涛¹^,², 吴珉¹^,², 吴声敢¹^,², 赵学平¹^,²^,^*()

1.浙江省农业科学院农产品质量安全与营养研究所,浙江杭州 310021
2.农产品质量安全危害因子与风险防控国家重点实验室,浙江杭州 310021

收稿日期:2023-03-06 出版日期:2024-02-25 发布日期:2024-03-05
作者简介:俞瑞鲜(1982—),女,浙江温岭人,硕士,助理研究员,研究方向为农药应用及环境安全评价。E-mail:yuruixian@163.com
通讯作者: *赵学平,E-mail:zhaoxueping@tom.com

Effect of sublethal dosage of fenvalerate to growth and development of the silkworm, Bombyx mori

YU Ruixian¹^,²(), HU Xiuqing¹^,², LIU Xinju¹^,², TANG Tao¹^,², WU Min¹^,², WU Shenggan¹^,², ZHAO Xueping¹^,²^,^*()

1. Institute of Agro-Product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
2. State Key Laboratory for Managing Biotic and Chemical Threats to Quality and Safety of Agro-Products, Hangzhou 310021, China

Received:2023-03-06 Online:2024-02-25 Published:2024-03-05

摘要/Abstract

摘要：

采用浸叶法测定了氰戊菊酯对家蚕的急性毒性和生长发育毒性,为农桑混栽区害虫防治药剂选择提供参考。急性毒性试验结果表明:氰戊菊酯对2龄起蚕的96 h-LC₅₀为0.251~0.570 mg·L^-1,属于高毒和剧毒级别。生长发育毒性试验结果表明:亚致死剂量(0.062 8、0.020 9、0.006 98、0.002 33、0.000 775 mg·L^-1)的氰戊菊酯处理对家蚕眠蚕体重和发育历期有一定的影响,但随着家蚕龄期的增长其影响逐渐减弱;0.002 33、0.000 775 mg·L^-1氰戊菊酯处理对家蚕化蛹率和死笼率有显著影响(P<0.05),对个体生长发育影响明显;0.062 8、0.020 9、0.006 98 mg·L^-1氰戊菊酯处理组家蚕茧层量显著低于对照组(P<0.05)。可见,氰戊菊酯对家蚕具有极高的急性毒性和慢性毒性效应。因此,在农桑混栽区使用氰戊菊酯时应注意,避免雾滴漂移或其他农事活动污染桑园引起家蚕中毒。

关键词: 氰戊菊酯, 家蚕, 亚致死效应, 生长发育毒性

Abstract:

In order to provide reference for choosing pesticides in controlling pests in agro-mulberry mixed planting areas, the acute toxicity and developmental toxicity of fenvalerate to silkworm were investigated by leaf dipping method. The results of acute toxicity showed that the 96 h-LC₅₀ for the 2nd instar larvae to fenvalerate was 0.251-0.570 mg·L^-1, belonging to high and extreme level toxicity. The results of developmental toxicity showed that after the treatments at the sublethal dosages (0.062 8, 0.020 9, 0.006 98, 0.002 33 and 0.000 775 mg·L^-1) of fenvalerate, the development duration and body weight of molting larvae were affected, and the influence gradually became less abvious with instar. The rate of pupation and dead cocoons of 0.002 33 and 0.000 775 mg·L^-1 fenvalerate treatment groups were significantly affected (P<0.05), which significantly affected the individual growth and development. The cocoon shell weight of 0.062 8, 0.020 9 and 0.006 98 mg·L^-1 fenvalerate treatment groups were significantly lower than that of the control group (P<0.05). Results showed that fenvalerate had high acute and chronic toxic effects on silkworms. Therefore, when using fenvalerate in agro-mulberry mixed planting areas, mulberry pollution from droplet drift or other agricultural activities should be avoided.

Key words: fenvalerate, Bombyx mori, sublethal effect, growth and developmental toxicity

中图分类号:

S482.35

俞瑞鲜, 胡秀卿, 柳新菊, 汤涛, 吴珉, 吴声敢, 赵学平. 亚致死剂量氰戊菊酯对家蚕生长发育的影响[J]. 浙江农业学报, 2024, 36(2): 264-271.

YU Ruixian, HU Xiuqing, LIU Xinju, TANG Tao, WU Min, WU Shenggan, ZHAO Xueping. Effect of sublethal dosage of fenvalerate to growth and development of the silkworm, Bombyx mori[J]. Acta Agriculturae Zhejiangensis, 2024, 36(2): 264-271.

图/表 5

参考文献 43

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家蚕品种 Silkworm variety	时间 Time/h	毒力回归曲线 Toxicity regressive equation	LC₅₀/(mg·L^-1)	95%可置信限 95% FI/(mg·L^-1)	R²
芙蓉Furong	72	Y=5.807+6.249X	0.743	0.687~0.828	0.896
	96	Y=7.137+8.757X	0.570	0.469~0.667	0.905
湘辉Xianghui	72	Y=10.974+13.038X	0.348	0.315~0.411	0.893
	96	Y=11.415+13.085X	0.323	0.299~0.366	0.918
皓月Haoyue	72	Y=8.406+6.491X	0.299	0.257~0.331	0.958
	96	Y=8.745+6.239X	0.251	0.179~0.299	0.916

家蚕品种 Silkworm variety	时间 Time/h	毒力回归曲线 Toxicity regressive equation	LC₅₀/(mg·L^-1)	95%可置信限 95% FI/(mg·L^-1)	R²
芙蓉Furong	72	Y=5.807+6.249X	0.743	0.687~0.828	0.896
	96	Y=7.137+8.757X	0.570	0.469~0.667	0.905
湘辉Xianghui	72	Y=10.974+13.038X	0.348	0.315~0.411	0.893
	96	Y=11.415+13.085X	0.323	0.299~0.366	0.918
皓月Haoyue	72	Y=8.406+6.491X	0.299	0.257~0.331	0.958
	96	Y=8.745+6.239X	0.251	0.179~0.299	0.916

氰戊菊酯浓度 Fenvalerate concentration/(mg·L^-1)	幼虫历期 Larval duration/h
氰戊菊酯浓度 Fenvalerate concentration/(mg·L^-1)	2龄2nd instar	3龄3rd instar	4龄4th instar	5龄5th instar
0.062 8	75.33±0.63 c	87.67±2.41 ab	100.07±3.01 ab	173.63±2.13 a
0.020 9	76.67±0.93 c	85.80±4.17 ab	101.73±1.40 a	180.37±3.20 a
0.006 98	79.83±4.49 bc	83.30±3.72 b	101.00±1.23 a	176.53±6.65 a
0.002 33	77.90±2.77 c	85.73±1.42 ab	100.03±1.72 ab	172.80±1.04 a
0.000 775	76.97±1.69 c	90.57±1.35 a	96.37±0.894 b	173.97±2.86 a
0(空白Control)	85.70±3.12 a	83.17±3.55 b	103.13±2.67 a	172.87±4.08 a
0(助剂Adjuvant)	83.30±1.99 ab	83.80±1.77 b	100.43±1.14 a	176.30±4.09 a

氰戊菊酯浓度 Fenvalerate concentration/(mg·L^-1)	幼虫历期 Larval duration/h
氰戊菊酯浓度 Fenvalerate concentration/(mg·L^-1)	2龄2nd instar	3龄3rd instar	4龄4th instar	5龄5th instar
0.062 8	75.33±0.63 c	87.67±2.41 ab	100.07±3.01 ab	173.63±2.13 a
0.020 9	76.67±0.93 c	85.80±4.17 ab	101.73±1.40 a	180.37±3.20 a
0.006 98	79.83±4.49 bc	83.30±3.72 b	101.00±1.23 a	176.53±6.65 a
0.002 33	77.90±2.77 c	85.73±1.42 ab	100.03±1.72 ab	172.80±1.04 a
0.000 775	76.97±1.69 c	90.57±1.35 a	96.37±0.894 b	173.97±2.86 a
0(空白Control)	85.70±3.12 a	83.17±3.55 b	103.13±2.67 a	172.87±4.08 a
0(助剂Adjuvant)	83.30±1.99 ab	83.80±1.77 b	100.43±1.14 a	176.30±4.09 a

氰戊菊酯浓度 Fenvalerate concentration/ (mg·L^-1)	全茧量 Cocoon weight/g	茧层量 Cocoon shell weight/g	蛹重 Pupa weight/g	幼虫死亡率 Larvae mortality/%	结茧率 Cocooning rate/%	化蛹率 Pupation rate/%	死笼率 Rate of dead cocoon/%
0.062 8	1.660±0.044 1 a	0.335±0.005 8 b	1.316±0.025 1 a	4.44 a	95.56 a	83.76 bc	16.24 bc
0.020 9	1.656±0.014 9 a	0.335±0.008 1 b	1.311±0.030 1 a	2.22 a	97.78 a	88.73 abc	11.27 bcd
0.006 98	1.687±0.025 3 a	0.339±0.013 1 b	1.339±0.020 7 a	6.67 a	93.33 a	88.39 abc	11.61 bcd
0.002 33	1.727±0.078 2 a	0.355±0.016 5 ab	1.362±0.055 5 a	4.44 a	95.56 a	79.21 c	18.41 b
0.000 775	1.691±0.037 1 a	0.356±0.014 4 ab	1.325±0.038 7 a	4.44 a	95.56 a	64.92 d	35.08 a
0(空白Control)	1.760±0.016 6 a	0.366±0.002 6 a	1.387±0.020 1 a	2.22 a	97.78 a	95.56 a	4.44 d
0(助剂Adjuvant)	1.703±0.146 4 a	0.363±0.010 0 a	1.339±0.153 9 a	2.22 a	97.78 a	93.17 ab	6.83 cd

亚致死剂量氰戊菊酯对家蚕生长发育的影响

Effect of sublethal dosage of fenvalerate to growth and development of the silkworm, Bombyx mori

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