两种树龄腾冲红花油茶花梗内生真菌的多样性

doi:10.3969/j.issn.1004-1524.20230757

浙江农业学报 ›› 2024, Vol. 36 ›› Issue (5): 1076-1085.DOI: 10.3969/j.issn.1004-1524.20230757

两种树龄腾冲红花油茶花梗内生真菌的多样性

黄融¹(), 孟庆鑫¹, 吴晓漫¹, 荀利杰¹, 张俊丽¹, 董霞², 董坤¹^,^*(), 龚雪阳¹^,^*()

1.云南农业大学动物科学技术学院/东方蜜蜂研究所/云南省蜜蜂资源可持续利用工程研究中心,云南昆明 650201
2.云南农业大学食品科技学院,云南昆明 650201

收稿日期:2023-06-13 出版日期:2024-05-25 发布日期:2024-05-29
作者简介:黄融(1998—),女,云南大理人,硕士研究生,主要从事蜜粉源植物授粉研究。E-mail:huangrongg2022@163.com
通讯作者: * 董坤,E-mail:dongkun19722004@aliyun.com;龚雪阳,E-mail:xueyangg11@126.com
基金资助:
国家自然科学基金项目(32060241);国家现代产业技术体系(蜜蜂)项目(CARS-44-KXJ13);云南省教育厅科学研究基金项目(2023Y0980);云南农业大学科研启动基金项目(KY2018-37)

Diversity analysis of endophytic fungi in the pedicels of two tree-age Camellia reticulate

HUANG Rong¹(), MENG Qingxin¹, WU Xiaoman¹, XUN Lijie¹, ZHANG Junli¹, DONG Xia², DONG Kun¹^,^*(), GONG Xueyang¹^,^*()

1. Faculty of Animal Science and Technology, Yunnan Agricultural University/Eastern Bee Research Institute, Yunnan Agricultural University/Yunnan Provincial Engineering and Research Center for Sustainable Utilization of Honeybee Resources, Kunming 650201,China
2. College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China

Received:2023-06-13 Online:2024-05-25 Published:2024-05-29

摘要/Abstract

摘要：

以腾冲红花油茶花梗为试材,采用高通量测序的方法,研究了12年生(N组)和60年生(O组)树龄的腾冲红花油茶花梗内生真菌群落结构及其多样性。结果表明,两种树龄腾冲红花油茶花梗内生真菌OTU和Alpha分析结果存在差异,物种多样性和丰富度差异显著。在两种树龄腾冲红花油茶花梗中子囊菌门和担子菌门是优势菌门(二者丰度之和>90%),但目、科水平上两组样品优势种群差异巨大,很多物种(真菌)只能分类到目水平,其中O组的煤炱目未分类科和N组银耳目未分类科占比最大。内生真菌功能预测得到11个生态共位群,未知(Unknown)占比最高,动物病原菌、植物病原菌、真菌寄生菌等与各种腐生菌组成了多功能的营养型,内生真菌中病原菌、寄生菌和腐生菌的丰度受树龄影响较大。综上所述,树龄大的腾冲红花油茶花梗中内生真菌多样性和丰富度显著增多,并且优势真菌种类、丰度和功能也发生改变,因此,利用内生真菌时树龄因素不可忽视。

关键词: 腾冲红花油茶, 树龄, 内生真菌多样性, 高通量测序

Abstract:

Taking the pedicel of Camellia reticulata as the test material, the community structure and diversity of endophytic fungi in the pedicel of Camellia reticulata at 12 and 60 years old were studied by high-throughput sequencing. The analysis results showed that there were differences in OTU and Alpha results of endophytic fungi in the pedicels of Camellia reticulata of two tree ages, and there were significant differences in species diversity and richness. Ascomycota and Basidiomycota were the dominant phyla (the sum of their abundance>90%) in the pedicels of Camellia reticulata of two tree ages, but the dominant populations of the two groups of samples at the level of order and family were very different. Many species (fungi) can only be classified to the level of order, among which unclassified_o_Capnodiales of the O group and unclassified_o_Tremellales of the N group accounted for a large proportion. The function of endophytic fungi was predicted to obtain 11 ecological symbionts, with the highest proportion of unknown. Animal pathogens, plant pathogens, fungal parasites, and various saprophytic bacteria formed a multifunctional nutrient type. The abundance of pathogens, parasites, and saprophytic bacteria in endophytic fungi was greatly affected by tree age. In summary, the diversity and richness of endophytic fungi in the pedicels of Camellia reticulata increased significantly, and the species, abundance, and function of dominant fungi also changed. Therefore, the age factor cannot be ignored when using endophytic fungi.

Key words: Camellia reticulate, tree age, diversity of endophytic fungi, high-throughput sequencing

中图分类号:

S794.4

黄融, 孟庆鑫, 吴晓漫, 荀利杰, 张俊丽, 董霞, 董坤, 龚雪阳. 两种树龄腾冲红花油茶花梗内生真菌的多样性[J]. 浙江农业学报, 2024, 36(5): 1076-1085.

HUANG Rong, MENG Qingxin, WU Xiaoman, XUN Lijie, ZHANG Junli, DONG Xia, DONG Kun, GONG Xueyang. Diversity analysis of endophytic fungi in the pedicels of two tree-age Camellia reticulate[J]. Acta Agriculturae Zhejiangensis, 2024, 36(5): 1076-1085.

图/表 12

表1 两种树龄腾冲红花油茶花梗内生真丰富度比较

Table 1 Comparison of richness of endophytic fungi in pedicels of Camellia reticulate of two tree ages

样本 Sample	OTU数 Number of OTU	门 Phylum	纲 Class	目 Order	科 Family	属 Genus
O组Group O	1 032	4	22	68	150	240
N组Group N	528	4	21	65	123	185

图1 OTU水平Venn图分析按树龄分为N组(12 a)和O组(60 a)。下同。

Fig.1 Venn analysis on OTU level The experimental samples were divided into two groups according to tree age: group N (12 a) and group O (60 a). The same as below.

图2 两种树龄的腾冲红花油茶花梗内生真菌Sobs稀释曲线(A)和Shannon指数稀释曲线(B)

Fig.2 Rarefaction curves of Sobs index (A) and Shannon index (B) of endophytic fungi detected in pedicels of Camellia reticulate of two tree ages

表2 两种树龄腾冲红花油茶花梗内生真菌多样性指数

Table 2 Endophytic fungi diversity indexes in pedicels of Camellia reticulate of two tree ages

样本 Sample	Sobs指数 Sobs index	Shannon指数 Shannon index	Simpson指数 Simpson index	Chao1指数 Chao1 index	ACE指数 ACE index	覆盖度 Coverage/%
O组Group O	530.33 ± 53.17 a	3.64 ± 0.29 a	0.08 ± 0.02 b	619.90 ± 87.17 a	373.90 ± 35.85 a	99.82
N组Group N	283.67 ± 45.12 b	2.29 ± 0.37 b	0.20 ± 0.05 a	343.70 ± 38.53 b	617.80 ±38.53 b	99.88

图3 两种树龄腾冲红花油茶花梗内生真菌群落在门水平上的群落多样性

Fig.3 Relative abundance of endophytic fungi at phylum level in pedicels of Camellia reticulate of two tree ages

图4 两种树龄腾冲红花油茶花梗内生真菌群落在目水平上的群落多样性

Fig.4 Relative abundance of endophytic fungi at order level in pedicels of Camellia reticulate of two tree ages

图5 两种树龄的腾冲红花油茶花梗内生真菌群落在科水平上的群落多样性

Fig.5 Relative abundance of endophytic fungi at family level in pedicels of Camellia reticulate of two tree ages

图6 两种树龄的腾冲红花油茶花梗内生真菌群落在门水平上的丰度差异比较

Fig.6 Comparison of differences in abundance of endophytic fungi at phylum level in pedicels of Camellia reticulate of two tree ages

图7 两种树龄的腾冲红花油茶花梗内生真菌群落在科水平上的丰度差异比较

Fig.7 Comparison of differences in abundance of endophytic fungi at family level in pedicels of Camellia reticulate of two tree ages

图8 两种树龄腾冲红花油茶花梗内生真菌群落PCoA图

Fig.8 PCoA diagram of endophytic fungi community in pedicels of Camellia reticulate of two tree ages

图9 两种树龄腾冲红花油茶花梗内生真菌群落聚类树

Fig.9 Clustering tree of endophytic fungi community in pedicels of Camellia reticulate of two tree ages

图10 两种树龄腾冲红花油茶花梗内生真菌功能预测

Fig.10 Function prediction of endophytic fungi from pedicels of Camellia reticulate of two tree ages

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两种树龄腾冲红花油茶花梗内生真菌的多样性

Diversity analysis of endophytic fungi in the pedicels of two tree-age Camellia reticulate

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