促生菌对上海青(Brassica rapa subsp. chinensis)降解噻虫嗪的影响

doi:10.3969/j.issn.1004-1524.20240093

浙江农业学报 ›› 2025, Vol. 37 ›› Issue (2): 394-404.DOI: 10.3969/j.issn.1004-1524.20240093

促生菌对上海青(Brassica rapa subsp. chinensis)降解噻虫嗪的影响

胡睿¹^,²(), 马丽雅², 万群², 王亚², 曹瑶瑶², 邵思城¹, 葛静², 吴祥为¹^,^*(), 余向阳²^,^*()

1.安徽农业大学资源与环境学院,安徽合肥 230031
2.江苏省农业科学院农产品质量安全与营养研究所,省部共建国家重点实验室培育基地-江苏省食品质量安全重点实验室,江苏南京 210014

收稿日期:2024-01-28 出版日期:2025-02-25 发布日期:2025-03-20
作者简介:余向阳,E-mail:yuxy@jaas.ac.cn
*吴祥为,E-mail:wxw@ahau.edu.cn;
胡睿(2000—),女,安徽安庆人,硕士研究生,主要从事环境微生物研究。E-mail:hurui001008@163.com
通讯作者: 吴祥为,余向阳
基金资助:
国家自然科学基金(22206064);国家自然科学基金(32302406)

Effect of growth-promoting bacteria on the degradation of thiamethoxam in Brassica rapa subsp. chinensis

HU Rui¹^,²(), MA Liya², WAN Qun², WANG Ya², CAO Yaoyao², SHAO Sicheng¹, GE Jing², WU Xiangwei¹^,^*(), YU Xiangyang²^,^*()

1. College of Resources and Environment, Anhui Agricultural University, Hefei 230031, China
2. Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology, Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China

Received:2024-01-28 Online:2025-02-25 Published:2025-03-20
Contact: WU Xiangwei,YU Xiangyang

摘要/Abstract

摘要：

为探讨促生菌及其复合菌群对上海青(Brassica rapa subsp. chinensis)降解噻虫嗪的影响,以假单胞菌NS6、肠杆菌NS54和NS62、嗜水气单胞菌NS69为材料,采用拮抗试验测定菌株间的相容性,结合促生特性实验,筛选构建复合菌群的菌株;通过盆栽试验,研究促生单菌株与复合菌群对上海青生长、品质和降解噻虫嗪的影响。结果表明,各菌株间不存在拮抗关系,且NS54+NS62+NS69复合菌群促生效果最好。盆栽结果显示,单菌株和最佳复合菌群处理均能提高上海青的株高、根长、鲜重、干重和叶绿素含量,有效降低噻虫嗪处理后上海青的MDA含量,同时提高上海青的可溶性糖、可溶性蛋白和维生素C含量,且复合菌群的处理效果优于单菌株。此外,NS54+NS62+NS69复合菌群能上调上海青中抗逆植物激素(水杨酸和茉莉酸)相关基因的表达,从而促进上海青中噻虫嗪的降解。综上,NS54+NS62+NS69复合菌群在促进上海青生长、提高上海青品质的同时,有效降低了上海青中噻虫嗪的残留量。研究结果为保障农产品质量安全、开发功能微生物菌剂提供了重要的理论基础。

关键词: 促生菌, 复合菌群, 噻虫嗪, 上海青, 促生, 降解

Abstract:

To explore the impact of plant growth-promoting bacteria and their combined consortia on the degradation of thiamethoxam in Brassica rapa subsp. chinensis, this study used Pseudomonas sp. (NS6), Enterobacter sp. (NS54 and NS62), and Aeromonas hydrophila(NS69) as materials. Antagonistic tests were conducted to determine the compatibility between the strains, and based on plant growth-promoting characteristics experiments, the strains for constructing the composite microbial community were screened. Through pot experiments, the effects of single plant growth-promoting bacterium and the best-performing microbial consortium on the growth, quality, and thiamethoxam degradation in B. rapa subsp. chinensis were studied. The results showed that there was no antagonistic relationship among the strains, and the NS54+NS62+NS69 microbial consortium exhibited the best plant growth-promoting performance. The pot experiment results revealed that both the single strain treatments and the optimal microbial consortium treatment could improve the plant height, root length, fresh weight, dry weight, and chlorophyll content of B. rapa subsp. chinensis. They effectively reduced the malondialdehyde (MDA) content after thiamethoxam treatment and increased the contents of soluble sugars, soluble proteins, and vitamin C in B. rapa subsp. chinensis, with the microbial consortium treatment being superior to that of the single strain treatments. Additionally, the NS54+NS62+NS69 microbial consortium upregulated the expression of stress-responsive plant hormones (salicylic acid and jasmonic acid) related genes in B. rapa var. chinensis, thereby promoting the degradation of thiamethoxam. In summary, the NS54+NS62+NS69 microbial consortium not only promoted the growth and improved the quality of B. rapa subsp. Chinensis, but also effectively reduced the residual amount of thiamethoxam in the plant. This study result could provide an important theoretical foundation for ensuring the quality and safety of agricultural products and developing functional microbial agents

Key words: growth-promoting bacterium, microbial consortium, thiamethtoxam, Brassica rapa subsp. chinensis, growth-promotion, degradation

中图分类号:

S481.8

胡睿, 马丽雅, 万群, 王亚, 曹瑶瑶, 邵思城, 葛静, 吴祥为, 余向阳. 促生菌对上海青(Brassica rapa subsp. chinensis)降解噻虫嗪的影响[J]. 浙江农业学报, 2025, 37(2): 394-404.

HU Rui, MA Liya, WAN Qun, WANG Ya, CAO Yaoyao, SHAO Sicheng, GE Jing, WU Xiangwei, YU Xiangyang. Effect of growth-promoting bacteria on the degradation of thiamethoxam in Brassica rapa subsp. chinensis[J]. Acta Agriculturae Zhejiangensis, 2025, 37(2): 394-404.

图/表 9

参考文献 38

[1]	HLADIK M L, MAIN A R, GOULSON D. Environmental risks and challenges associated with neonicotinoid insecticides[J]. Environmental Science & Technology, 2018, 52(6): 3329-3335.
[2]	ZHAN H L, WAN Q, WANG Y, et al. An endophytic bacterial strain, Enterobacter cloacae TMX-6, enhances the degradation of thiamethoxam in rice plants[J]. Chemosphere, 2021, 269: 128751.
[3]	JESCHKE P, NAUEN R, SCHINDLER M, et al. Overview of the status and global strategy for neonicotinoids[J]. Journal of Agricultural and Food Chemistry, 2011, 59(7): 2897-2908.
[4]	WU R L, HE W, LI Y L, et al. Residual concentrations and ecological risks of neonicotinoid insecticides in the soils of tomato and cucumber greenhouses in Shouguang, Shandong Province, East China[J]. Science of the Total Environment, 2020, 738: 140248.
[5]	MONTIEL-LEÓN J M, DUY S V, MUNOZ G, et al. Occurrence of pesticides in fruits and vegetables from organic and conventional agriculture by QuEChERS extraction liquid chromatography tandem mass spectrometry[J]. Food Control, 2019, 104: 74-82.
[6]	谭颖, 张琪, 赵成, 等. 蔬菜水果中的新烟碱类农药残留量与人群摄食暴露健康风险评价[J]. 生态毒理学报, 2016, 11(6): 67-81.
	TAN Y, ZHANG Q, ZHAO C, et al. Residues of neonicotinoid pesticides in vegetables and fruit and health risk assessment of human exposure via food intake[J]. Asian Journal of Ecotoxicology, 2016, 11(6): 67-81. (in Chinese with English abstract)
[7]	MITCHELL E A D, MULHAUSER B, MULOT M, et al. A worldwide survey of neonicotinoids in honey[J]. Science, 2017, 358(6359): 109-111.
[8]	GREEN T, TOGHILL A, LEE R, et al. Thiamethoxam induced mouse liver tumors and their relevance to humans. part 1: mode of action studies in the mouse[J]. Toxicological Sciences, 2005, 86(1): 36-47.
[9]	HAN W C, TIAN Y, SHEN X M. Human exposure to neonicotinoid insecticides and the evaluation of their potential toxicity: an overview[J]. Chemosphere, 2018, 192: 59-65.
[10]	代金霞, 田平雅, 沈聪, 等. 耐盐植物根际促生菌筛选及促生效应研究[J]. 生态环境学报, 2021, 30(5): 968-975.
	DAI J X, TIAN P Y, SHEN C, et al. Screening of rhizosphere bacteria from salt tolerant plants and their growth promoting effects[J]. Ecology and Environmental Sciences, 2021, 30(5): 968-975. (in Chinese with English abstract)
[11]	ZHOU X G, ZHANG X H, MA C L, et al. Biochar amendment reduces cadmium uptake by stimulating cadmium-resistant PGPR in tomato rhizosphere[J]. Chemosphere, 2022, 307: 136138.
[12]	郑培峰, 姜小蕾, 翟彦霖, 等. PGPR对莠去津污染土壤中结缕草生长及生理的影响[J]. 中国农学通报, 2022, 38(5): 124-131.
	ZHENG P F, JIANG X L, ZHAI Y L, et al. PGPR in atrazine contaminated soil: effect on the growth and physiology of Zoysia japonica Steud[J]. Chinese Agricultural Science Bulletin, 2022, 38(5): 124-131. (in Chinese with English abstract)
[13]	HE W, MEGHARAJ M, WU C Y, et al. Endophyte-assisted phytoremediation: mechanisms and current application strategies for soil mixed pollutants[J]. Critical Reviews in Biotechnology, 2020, 40(1): 31-45.
[14]	ISLAM F, YASMEEN T, ALI Q, et al. Influence of Pseudomonas aeruginosa as PGPR on oxidative stress tolerance in wheat under Zn stress[J]. Ecotoxicology and Environmental Safety, 2014, 104: 285-293.
[15]	ZHANG C L, YU Z P, ZHANG M Y, et al. Serratia marcescens PLR enhances lateral root formation through supplying PLR-derived auxin and enhancing auxin biosynthesis in Arabidopsis[J]. Journal of Experimental Botany, 2022, 73(11): 3711-3725.
[16]	刘悦, 徐伟慧, 王志刚. 大豆根际促生菌的筛选鉴定与促生效应[J]. 浙江农业学报, 2023, 35(12): 2775-2784.
	LIU Y, XU W H, WANG Z G. Screening and identification of soybean rhizosphere growth-promoting bacteria and their growth-promoting effects[J]. Acta Agriculturae Zhejiangensis, 2023, 35(12): 2775-2784. (in Chinese with English abstract)
[17]	翟凯辉, 张影影, 高夕全. 种子内生菌促生机制和抗病机理研究进展[J]. 农业生物技术学报, 2023, 31(9): 1965-1979.
	ZHAI K H, ZHANG Y Y, GAO X Q. Research progress on mechanisms of growth promotion and disease resistance of seed endophytes[J]. Journal of Agricultural Biotechnology, 2023, 31(9): 1965-1979. (in Chinese with English abstract)
[18]	宋歌, 才满, 杜克久. 具多氯联苯降解特性绦柳内生菌的分离筛选及其移除性能[J]. 生态学杂志, 2016, 35(4): 1038-1046.
	SONG G, CAI M, DU K J. Isolation and characterization of polychlorinated biphenyl-degrading endophytic bacterium from Salix matsudana f. pendula[J]. Chinese Journal of Ecology, 2016, 35(4): 1038-1046. (in Chinese with English abstract)
[19]	FENG F Y, CHEN X L, WANG Q, et al. Use of Bacillus-siamensis-inoculated biochar to decrease uptake of dibutyl phthalate in leafy vegetables[J]. Journal of Environmental Management, 2020, 253: 109636.
[20]	CHEN S, MA Z, LI S Y, et al. Colonization of polycyclic aromatic hydrocarbon-degrading bacteria on roots reduces the risk of PAH contamination in vegetables[J]. Environment International, 2019, 132: 105081.
[21]	张帆, 马丽雅, 冯发运, 等. 接种嗜水气单胞菌对水稻降解毒死蜱的影响及作用机制[J]. 农药学学报, 2023, 25(2): 422-434.
	ZHANG F, MA L Y, FENG F Y, et al. Effect and mechanism of inoculation with Aeromonas hydrophila on the degradation of chlorpyrifos in rice[J]. Chinese Journal of Pesticide Science, 2023, 25(2): 422-434. (in Chinese with English abstract)
[22]	张秀雨, 马秀花, 李玮, 等. 芽孢杆菌复合菌群的构建及其对野燕麦除草活性研究[J]. 南方农业学报, 2022, 53(12): 3444-3452.
	ZHANG X Y, MA X H, LI W, et al. Construction of Bacillus compound flora and its herbicidal activity against Avena fatua L[J]. Journal of Southern Agriculture, 2022, 53(12): 3444-3452. (in Chinese)
[23]	WANG W F, WAN Q, LI Y X, et al. Application of an endophyte Enterobacter sp. TMX13 to reduce thiamethoxam residues and stress in Chinese cabbage (Brassica chinensis L)[J]. Journal of Agricultural and Food Chemistry, 2020, 68(34): 9180-9187.
[24]	马莹, 王玥, 石孝均, 等. 植物促生菌在重金属生物修复中的作用机制及应用[J]. 环境科学, 2022, 43(9): 4911-4922.
	MA Y, WANG Y, SHI X J, et al. Mechanism and application of plant growth-promoting bacteria in heavy metal bioremediation[J]. Environmental Science, 2022, 43(9): 4911-4922. (in Chinese with English abstract)
[25]	HA-TRAN D M, NGUYEN T T M, HUNG S H, et al. Roles of plant growth-promoting rhizobacteria (PGPR) in stimulating salinity stress defense in plants: a review[J]. International Journal of Molecular Sciences, 2021, 22(6): 3154.
[26]	TRIVEDI P, LEACH J E, TRINGE S G, et al. Plant-microbiome interactions: from community assembly to plant health[J]. Nature Reviews Microbiology, 2020, 18(11): 607-621.
[27]	GLICK B R. Plant growth-promoting bacteria: mechanisms and applications[J]. Scientifica, 2012, 2012: 963401.
[28]	EKE P, KUMAR A, SAHU K P, et al. Endophytic bacteria of desert Cactus(Euphorbia trigonas Mill) confer drought tolerance and induce growth promotion in tomato (Solanum lycopersicum L.)[J]. Microbiological Research, 2019, 228: 126302.
[29]	GIAUQUE H, CONNOR E W, HAWKES C V. Endophyte traits relevant to stress tolerance, resource use and habitat of origin predict effects on host plants[J]. New Phytologist, 2019, 221(4): 2239-2249.
[30]	WITTEBOLLE L, MARZORATI M, CLEMENT L, et al. Initial community evenness favours functionality under selective stress[J]. Nature, 2009, 458(7238): 623-626.
[31]	车永梅, 刘广超, 郭艳苹, 等. 一种耐盐复合菌剂的制备和促生作用研究[J]. 生物技术通报, 2023, 39(11): 217-225.
	CHE Y M, LIU G C, GUO Y P, et al. Preparation of compound halotolerant bioinoculant and study on its growth-promoting effect[J]. Biotechnology Bulletin, 2023, 39(11): 217-225. (in Chinese with English abstract)
[32]	潘宇, 张昊, 李湘, 等. 耐盐促生菌与其复合菌剂对盐胁迫狼尾草生长及生理生化的影响[J]. 贵州农业科学, 2023, 51(7): 39-49.
	PAN Y, ZHANG H, LI X, et al. Effect of salt-tolerant and growth-promoting bacteria and composite microbial agent on growth and physicoch-emical of Pennisetum alopecuroides under salt stress[J]. Guizhou Agricultural Sciences, 2023, 51(7): 39-49. (in Chinese with English abstract)
[33]	张希平, 田菊, 木其尔, 等. 基于代谢组学研究噻虫嗪和戊唑醇农药对蔬菜生理代谢的影响[J]. 沈阳农业大学学报, 2022, 53(6): 693-700.
	ZHANG X P, TIAN J, MU Q E, et al. Metabolomics reveals the effects of pesticide thiamethoxam and pentazolol on the physiological metabolism of vegetable[J]. Journal of Shenyang Agricultural University, 2022, 53(6): 693-700. (in Chinese with English abstract)
[34]	张亚, 师杨杰, 姚均伟, 等. 村镇有机生活垃圾处理产物对青菜品质及土壤性质的影响[J]. 江苏农业学报, 2023, 39(1): 88-96.
	ZHANG Y, SHI Y J, YAO J W, et al. Effects of the products from treatment of organic domestic waste in villages and towns on the quality of Brassica chinensis and soil properties[J]. Jiangsu Journal of Agricultural Sciences, 2023, 39(1): 88-96. (in Chinese with English abstract)
[35]	余冬冬, 李永军. 不同微生物菌剂对草莓灰霉病的防治效果、植株生长及果实品质的影响[J]. 浙江农业科学, 2023, 64(10): 2482-2486.
	YU D D, LI Y J. Effects of different microbial agents on the control of gray mold, plant growth, and fruit quality of strawberry[J]. Journal of Zhejiang Agricultural Sciences, 2023, 64(10): 2482-2486. (in Chinese with English abstract)
[36]	李婷, 张静, 曲明山, 等. 浇灌不同促生菌剂对网纹甜瓜生长发育及果实品质的影响[J]. 北方园艺, 2023(21): 36-42.
	LI T, ZHANG J, QU M S, et al. Effects of different growth-promoting agents on growth and fruit quality of netted melon[J]. Northern Horticulture, 2023(21): 36-42. (in Chinese with English abstract)
[37]	勾宇春, 王宗抗, 张志鹏, 等. 植物根际促生菌作用机制研究进展[J]. 应用与环境生物学报, 2023, 29(2): 495-506.
	GOU Y C, WANG Z K, ZHANG Z P, et al. Advance in role mechanisms of plant growth-promoting rhizobacteria[J]. Chinese Journal of Applied and Environmental Biology, 2023, 29(2): 495-506. (in Chinese with English abstract)
[38]	ALIZADEH H, BEHBOUDI K, AHMADZADEH M, et al. Induced systemic resistance in cucumber and Arabidopsis thaliana by the combination of Trichoderma harzianum Tr6 and Pseudomonas sp. Ps14[J]. Biological Control, 2013, 65(1): 14-23.

基因 Gene	正向引物序列 Forward primer sequence(5'→3')	反向引物序列 Reverse primer sequence(5'→3')
BraA05g023030.3C	GGCTGGACCTACGCTATA	CGCAACACCTTCTTACGA
BraA03g024150.3C	ACCAATCCGCCATCTACT	TCACCGTCCACCATTCT
BraA03g042180.3C	TCGGAGGAACAAGTAGAGG	CCATCAGGCAACAGAACTC
BraA03g021140.3C	ACTTCCGCCTCGTCTTACTC	AGCCGCCTTGTCTGAGATAG
BraA09g044270.3C	CGCTATGGCTTCGTATTGC	GTTCTTCACCGTCTGTCTC
BraA05g036420.3C	CCTAAGCAGCCTCACTAAC	CACTCGGTCCACACTCTT
BraA07g016780.3C	GAGAGGAAGAGACTCATTGC	GCTCACCAACCGTAACTC
BraA07g028130.3C	GTTCTTCCGTGACCTTGAC	GAGTAGCAGCCAACATTGAG
BraA08g009510.3C	GGCTCTGGAATCATTGGAC	CCGACACAACCGCATTAG
BraA10g033710.3C	AGCGGAGGAGGCAATAGT	CGAATACGGCAGTGTCAAC
BraA05g007500.3C	ATGGAGAAGAGGCGATAGTC	CTGAACGAGTAAGGCAACG
BraA07g025310.3C	GGATGGTGGTGAGTGATG	CAAGTGGAGGCGATAGCA
BraA07g029730.3C	GCTCTGTAAGGTGGTGGA	GGCGTATGGATTGTTGGT
BraA04g019480.3C	CGTAGCCTCTTCTCTTCAG	CCTTAACCATCTCACCTCTC
BraA05g032610.3C	TCGGAGATTGGTCGGTGGT	ATGGAGAGCAGGATGAGTG

基因 Gene	正向引物序列 Forward primer sequence(5'→3')	反向引物序列 Reverse primer sequence(5'→3')
BraA05g023030.3C	GGCTGGACCTACGCTATA	CGCAACACCTTCTTACGA
BraA03g024150.3C	ACCAATCCGCCATCTACT	TCACCGTCCACCATTCT
BraA03g042180.3C	TCGGAGGAACAAGTAGAGG	CCATCAGGCAACAGAACTC
BraA03g021140.3C	ACTTCCGCCTCGTCTTACTC	AGCCGCCTTGTCTGAGATAG
BraA09g044270.3C	CGCTATGGCTTCGTATTGC	GTTCTTCACCGTCTGTCTC
BraA05g036420.3C	CCTAAGCAGCCTCACTAAC	CACTCGGTCCACACTCTT
BraA07g016780.3C	GAGAGGAAGAGACTCATTGC	GCTCACCAACCGTAACTC
BraA07g028130.3C	GTTCTTCCGTGACCTTGAC	GAGTAGCAGCCAACATTGAG
BraA08g009510.3C	GGCTCTGGAATCATTGGAC	CCGACACAACCGCATTAG
BraA10g033710.3C	AGCGGAGGAGGCAATAGT	CGAATACGGCAGTGTCAAC
BraA05g007500.3C	ATGGAGAAGAGGCGATAGTC	CTGAACGAGTAAGGCAACG
BraA07g025310.3C	GGATGGTGGTGAGTGATG	CAAGTGGAGGCGATAGCA
BraA07g029730.3C	GCTCTGTAAGGTGGTGGA	GGCGTATGGATTGTTGGT
BraA04g019480.3C	CGTAGCCTCTTCTCTTCAG	CCTTAACCATCTCACCTCTC
BraA05g032610.3C	TCGGAGATTGGTCGGTGGT	ATGGAGAGCAGGATGAGTG

组合编号 Combination number	产IAA量 IAA production/(mg·L^-1)	有效磷含量 Vailable phosphorus/(mg·L^-1)	铁载体活性 Iron carrier activity/%	固氮 Nitrogen fixation
NS6	83.16±0.01 a	133.9±0.1 j	5.16±0.14 k	-
NS54	71.09±0.10 bcd	167.3±2.1 e	22.73±0.79 h	-
NS62	70.96±0.08 bcd	170.1±1.0 d	25.69±0.73 f	-
NS69	59.37±0.05 e	53.3±0.1 m	41.40±0.31 a	+
C1:NS6+NS54	74.32±0.02 abc	155.7±0.3 h	18.42±0.45 j	-
C2:NS6+NS62	72.18±0.02 bcd	162.1±0.1 f	20.44±0.10 i	+
C3:NS6+NS69	69.41±0.03 bcd	94.3±0.1 l	19.64±0.28 ij	-
C4:NS54+NS62	79.10±0.05 a	183.9±0.2 a	31.35±0.49 c	-
C5:NS54+NS69	68.06±0.06 cde	151.0±0.6 h	26.42±0.32 e	+
C6:NS62+NS69	65.06±0.04 de	143.5±0.3 i	23.49±0.45 fg	+
C7:NS6+NS54+NS62	78.27±0.06 ab	174.5±0.5 c	28.52±0.29 d	-
C8:NS6+NS54+NS69	66.37±0.04 de	125.3±0.2 k	26.46±0.35 e	+
C9:NS54+NS62+NS69	76.43±0.05 abc	180.9±0.1 b	33.47±0.42 b	+
C10:NS6+NS54+NS62+NS69	75.29±0.01 abc	156.3±0.3 g	22.48±0.42 gh	+

组合编号 Combination number	产IAA量 IAA production/(mg·L^-1)	有效磷含量 Vailable phosphorus/(mg·L^-1)	铁载体活性 Iron carrier activity/%	固氮 Nitrogen fixation
NS6	83.16±0.01 a	133.9±0.1 j	5.16±0.14 k	-
NS54	71.09±0.10 bcd	167.3±2.1 e	22.73±0.79 h	-
NS62	70.96±0.08 bcd	170.1±1.0 d	25.69±0.73 f	-
NS69	59.37±0.05 e	53.3±0.1 m	41.40±0.31 a	+
C1:NS6+NS54	74.32±0.02 abc	155.7±0.3 h	18.42±0.45 j	-
C2:NS6+NS62	72.18±0.02 bcd	162.1±0.1 f	20.44±0.10 i	+
C3:NS6+NS69	69.41±0.03 bcd	94.3±0.1 l	19.64±0.28 ij	-
C4:NS54+NS62	79.10±0.05 a	183.9±0.2 a	31.35±0.49 c	-
C5:NS54+NS69	68.06±0.06 cde	151.0±0.6 h	26.42±0.32 e	+
C6:NS62+NS69	65.06±0.04 de	143.5±0.3 i	23.49±0.45 fg	+
C7:NS6+NS54+NS62	78.27±0.06 ab	174.5±0.5 c	28.52±0.29 d	-
C8:NS6+NS54+NS69	66.37±0.04 de	125.3±0.2 k	26.46±0.35 e	+
C9:NS54+NS62+NS69	76.43±0.05 abc	180.9±0.1 b	33.47±0.42 b	+
C10:NS6+NS54+NS62+NS69	75.29±0.01 abc	156.3±0.3 g	22.48±0.42 gh	+

处理 Treatment	丙二醛含量 MDA content/ (μmol·L^-1)	可溶性糖含量 Soluble sugar content/(g·kg^-1)	可溶性蛋白含量 Soluble protein content/(g·kg^-1)	维生素C含量 Vitamin C content/(mg·kg^-1)
TMX	3.86±0.25 a	9.35±0.51 c	11.17±0.73 c	79.3±6.3 c
NS54+TMX	2.78±0.15 bc	12.63±0.88 ab	13.57±0.30 a	106.6±9.3 ab
NS62+TMX	3.18±0.22 b	11.39±0.93 b	12.42±0.45 b	90.8±5.9 bc
NS69+TMX	2.65±0.42 cd	12.70±0.40 ab	13.03±0.37 ab	105.4±5.1 ab
C9+TMX	2.25±0.11 d	13.30±0.50 a	13.01±0.46 ab	113.7±6.1 a

促生菌对上海青(Brassica rapa subsp. chinensis)降解噻虫嗪的影响

Effect of growth-promoting bacteria on the degradation of thiamethoxam in Brassica rapa subsp. chinensis

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 9

参考文献 38

相关文章 15

编辑推荐

Metrics

本文评价

[1]	鲁子正钢, 朱立新, 季宏兵, 汪康. 鞘氨醇单胞菌修复土壤重金属污染研究进展[J]. 浙江农业学报, 2024, 36(5): 1208-1216.
[2]	杨乐, 李文杨, 骆建莉, 郑伟, 张毕阳, 王震, 阎腾飞. 地膜覆盖和自然生草对桃园土壤养分、pH及水热的影响[J]. 浙江农业学报, 2024, 36(12): 2719-2726.
[3]	谢晓杰, 许双燕, 王文凡, 杨健, 赵卓群, 王敏, 郑华宝. 畜禽养殖废弃物中抗生素的微生物降解研究进展[J]. 浙江农业学报, 2023, 35(8): 1975-1992.
[4]	田玉刚, 万素梅, 林皎, 陈国栋, 李浩, 胡宇凯, 李燕芳, 胡守林, 毛廷勇, 赵书珍. 不同地膜类型与灌溉量对棉花光合参数和产量、品质的影响[J]. 浙江农业学报, 2023, 35(7): 1523-1531.
[5]	朱燕, 魏佳, 许自龙, 林天宝, 杨升, 刘岩, 吕志强, 刘培刚. 促生长植物激素对桑树叶片衰老过程生理生化指标的影响[J]. 浙江农业学报, 2023, 35(6): 1278-1285.
[6]	刘悦, 徐伟慧, 王志刚. 大豆根际促生菌的筛选鉴定与促生效应[J]. 浙江农业学报, 2023, 35(12): 2775-2784.
[7]	黄东慧, 钟鹏, 王建丽, 胡云龙, 王志刚. 环境条件对Bacillus altitudinis LZP02生物膜形成的影响[J]. 浙江农业学报, 2022, 34(7): 1466-1473.
[8]	孙淑媛, 侯丽娜, 杨桂玲, 杜雨婷, 赵芸. 葡萄中几种常用防治绿盲蝽农药的残留动态与风险评估[J]. 浙江农业学报, 2022, 34(7): 1513-1518.
[9]	李丽艳, 谭海霞, 李婧, 王连龙, 杜迎辉, 徐志文. 耐盐促生芽孢杆菌的筛选及其对盐胁迫下燕麦生长的影响[J]. 浙江农业学报, 2022, 34(6): 1268-1276.
[10]	冯娟, 朱廷恒, 罗春萍, 杨佳玥, 祝思瑜, 李彤. 黄粉虫（Tenebrio molitor）肠道中聚乳酸塑料降解菌的筛选及其降解特性[J]. 浙江农业学报, 2022, 34(6): 1277-1287.
[11]	张鑫鹏, 王信, 孙健, 伊国云, 李松龄. 一株假单胞菌的分离鉴定及其在青海地区堆肥中的应用潜力[J]. 浙江农业学报, 2022, 34(2): 343-351.
[12]	王一然, 康志超, 朱国鹏, 王洋, 其格其, 于洪文. 耐低温玉米秸秆降解菌群的优化及其效果[J]. 浙江农业学报, 2022, 34(12): 2720-2727.
[13]	冯欣欣, 李凤兰, 徐永清, 李磊, 贺付蒙, 冯艳忠, 袁强, 刘娣. 新疆寒冷地区腐木中产纤维素酶菌株的筛选与低温产酶特性[J]. 浙江农业学报, 2021, 33(8): 1468-1476.
[14]	刘丹丹, 孙宛玉, 王鹤. 三株降解阿特拉津菌株的特性与固定载体分析[J]. 浙江农业学报, 2021, 33(6): 1078-1087.
[15]	李福艳, 刘晓玉, 颜静婷, 蔡燕飞. 三株产吲哚乙酸根际促生芽孢杆菌的筛选鉴定及其促生作用[J]. 浙江农业学报, 2021, 33(5): 873-884.