耐低温玉米秸秆降解菌群的优化及其效果

doi:10.3969/j.issn.1004-1524.2022.12.15

浙江农业学报 ›› 2022, Vol. 34 ›› Issue (12): 2720-2727.DOI: 10.3969/j.issn.1004-1524.2022.12.15

耐低温玉米秸秆降解菌群的优化及其效果

王一然¹^,²(), 康志超¹, 朱国鹏¹, 王洋³, 其格其³^,^*(), 于洪文¹^,^*()

1.中国科学院东北地理与农业生态研究所,吉林长春 130102
2.长春理工大学化学与环境工程学院,吉林长春 130022
3.兴安盟农牧局兴安盟农牧科学研究所,内蒙古兴安 137400

收稿日期:2022-03-22 出版日期:2022-12-25 发布日期:2022-12-26
通讯作者: 其格其,于洪文
作者简介:其格其,E-mail:535830909@qq.com
*于洪文,E-mail:yuhw@neigae.ac.cn;
王一然(1996—),女,吉林长春人,硕士研究生,研究方向为物理化学。E-mail:1142402148@qq.com
基金资助:
黑土地保护与利用科技创新工程专项(XDA28010502);中国科学院科研仪器设备研制项目(YJKYYQ20200013);吉林省科技发展计划(20210203007SF)

Optimization of low temperature resistant corn stalk degrading bacterial community and its effect

WANG Yiran¹^,²(), KANG Zhichao¹, ZHU Guopeng¹, WANG Yang³, QI Geqi³^,^*(), YU Hongwen¹^,^*()

1. Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, China
2. School of Chemistry and Environmental Engineering, Changchun University of Science and Technology, Changchun 130022, China
3. Hinggan League Institute of Agricultural and Animal Husbandry Sciences, Agriculture and Animal Husbandry Bureau of Hinggan League, Hinggan 137400, Inner Mongolia, China

Received:2022-03-22 Online:2022-12-25 Published:2022-12-26
Contact: QI Geqi,YU Hongwen

摘要/Abstract

摘要：

为适应中国东北地区年平均气温低、冻融频繁的气候特点,优化构建能在低温环境下高效降解秸秆的菌群,通过宏基因组测序分析原始耐低温秸秆降解菌群JZ5中鞘氨醇杆菌(Sphingobacter)、节杆菌(Arthrobacter)、黄杆菌(Flavobacterium)、地杆菌(Geobacter)、代尔夫特菌(Delfteia)和鞘氨醇单胞菌(Sphingosphinomonas)共6个菌属间的相关性,简化原始菌群JZ5组成。结果表明,优化后的菌群CJZ1、CJZ2在15 ℃培养12 d后,pH值相较初始值分别提高0.76和0.66,秸秆降解率较原始菌群分别提高0.47和0.25百分点,差异不显著;同时,CJZ1、CJZ2的漆酶、木质素过氧化物酶活性峰值显著下降,但纤维素酶活性峰值分别提高43.66%、37.99%,半纤维素酶活性峰值分别提高21.40%、12.91%,说明优化后的菌群玉米秸秆降解潜力显著升高。试验结果可为菌群的优化构建提供新的思路,并为低温环境下秸秆的高效降解提供优质菌群。

关键词: 低温, 秸秆, 菌群, 降解率, 木质素过氧化物酶, 纤维素酶, 半纤维素酶

Abstract:

In order to adapt to the climate characteristics of low average annual temperature and frequent freezing and thawing in northeast China, this study optimized the construction of microbial community which could efficiently degrade straw in low temperature environment. The metagenomic sequencing technology was employed for correlation analysis of six bacterial genera (Sphingobacter, Arthrobacter, Flavobacterium, Geobacter, Delfteia and Sphingosphinomonas) in JZ5, an original frigostable straw degrading bacterial community. Besides, the composition of the original community JZ5 was simplified. The results showed that after 12 d of culture at 15 ℃, pH value of optimized community CJZ1 and CJZ2 increased by 0.76 and 0.66 compared with the initial value, respectively, and the degradation rate of straw increased by 0.47 and 0.25 percentage point compared with the original community, respectively, with no significant difference. In addition, the active peak of laccase and lignin peroxidase significantly decreased, while cellulase activity increased by 43.66% and 37.99%, hemicellulase activity increase by 21.40% and 12.91%, respectively in CJZ1 and CJZ2. The results could provide a new idea for the optimization of bacterial community, and a high quality bacterial community for the efficient degradation of straw at low temperature.

Key words: low temperature, straw, bacterial community, degradation rate, Lignin peroxidase, cellulase, hemicellulase

中图分类号:

S141.4
X712

王一然, 康志超, 朱国鹏, 王洋, 其格其, 于洪文. 耐低温玉米秸秆降解菌群的优化及其效果[J]. 浙江农业学报, 2022, 34(12): 2720-2727.

WANG Yiran, KANG Zhichao, ZHU Guopeng, WANG Yang, QI Geqi, YU Hongwen. Optimization of low temperature resistant corn stalk degrading bacterial community and its effect[J]. Acta Agriculturae Zhejiangensis, 2022, 34(12): 2720-2727.

图/表 7

表1 菌群组成

Table 1 Bacteria composition

分组 Group	不同属的菌株数量Number of strains from different genera
分组 Group	鞘氨醇杆菌 Sphingobacter	代尔夫特菌 Delfteia	鞘氨醇单胞菌 Sphingosphinomonas	地杆菌 Geobacter	黄杆菌 Flavobacterium	节杆菌 Arthrobacter
JZ5	10	1	1	4	2	2
CJZ1	10	1	1	4	2	0
CJZ2	10	1	1	4	0	0

图1 不同菌群的pH值

Fig.1 pH of different bacterial communities

图2 不同菌群的漆酶活性

Fig.2 Laccase activity of different bacterial communities

图3 不同菌群木质素过氧化物酶活性

Fig.3 Lignin peroxidase activity of different bacterial communities

图4 不同菌群纤维素酶活性

Fig.4 Cellulase activity curve of different bacterial communities

图5 不同菌群半纤维素酶活性

Fig.5 Hemicellulase activity of different bacterial communities

图6 不同菌群的秸秆降解率

Fig.6 Degradation rate of straw by different bacterial communities

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耐低温玉米秸秆降解菌群的优化及其效果

Optimization of low temperature resistant corn stalk degrading bacterial community and its effect

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