低温弱光胁迫对设施黄瓜叶片面积与干物质量的影响

doi:10.3969/j.issn.1004-1524.2022.03.12

浙江农业学报 ›› 2022, Vol. 34 ›› Issue (3): 517-524.DOI: 10.3969/j.issn.1004-1524.2022.03.12

低温弱光胁迫对设施黄瓜叶片面积与干物质量的影响

唐卫东^a^,^b(), 刘振文^a^,^b, 刘冬生^a^,^b, 胡雪华^c

a. 井冈山大学农作物生长重点实验室,江西吉安 343009
b. 井冈山大学电子与信息工程学完,江西吉安 343009
c. 井冈山大学生命科学学院,江西吉安 343009

收稿日期:2021-05-14 出版日期:2022-03-25 发布日期:2022-03-30
作者简介:唐卫东(1974—),男,江西吉安人,博士,副教授,主要从事园艺栽培、植物生长建模与植物逆境调控研究。E-mail: metangwd@163.com
基金资助:
国家自然科学基金(31860574);江西省自然科学基金(20161BAB204172)

Effects of low temperature and weak light stress on leaf area and dry matter of cucumber in greenhouse

TANG Weidong^a^,^b(), LIU Zhenwen^a^,^b, LIU Dongsheng^a^,^b, HU Xuehua^c

a. Key Laboratory of Crop Growth, Jinggangshan University, Ji’an 343009, Jiangxi, China
b. College of Electronics and Information Engineering, Jinggangshan University, Ji’an 343009, Jiangxi, China
c. College of Life Sciences, Jinggangshan University, Ji’an 343009, Jiangxi, China

Received:2021-05-14 Online:2022-03-25 Published:2022-03-30

摘要/Abstract

摘要：

为研究黄瓜不同叶龄叶片的叶面积、干物质量在低温弱光胁迫下的变化规律,以黄瓜品种碧玉3号为试材,在温室无土栽培管理模式下,分别设置4个低温弱光水平,采用正交试验设计进行实验,测定不同叶龄叶片的叶面积、植株干物质量变化。结果表明:低温胁迫对叶龄小于15 d的叶片形态发育影响较大,与对照相比,叶片面积下降幅度较大;对叶龄为15~25 d的叶片影响相对较小;在中度低温(10~15 ℃)与弱光(光量子通量密度PPFD为100~200 μmol·m^-2·s^-1)胁迫下,处于伸展期(叶龄5~15 d)的叶片叶面积增长量明显减少,叶干物质量有所增加;而重度低温(5~10 ℃)与弱光(PPFD为0~100 μmol·m^-2·s^-1)条件下叶龄小于15 d的叶片形态发育基本停滞,与对照相比,叶片干物质量下降幅度较大;轻度低温弱光(15~20 ℃,PPFD为200~400 μmol·m^-2·s^-1)胁迫对黄瓜叶片的正常发育不构成显著威胁,尤其是对发育初期叶片面积增长与干物质量积累影响较小。研究结果表明,为保证叶片从发育初期至扩展期的正常生长,应避免中度以上低温弱光胁迫(5~15 ℃,PPFD为0~200 μmol·m^-2·s^-1)。

关键词: 黄瓜, 低温, 弱光, 叶片面积, 干物质

Abstract:

To study the change rules of leaf area and dry matter weight of cucumber with different leaf ages under low temperature and weak light stress, cucumber variety Biyu 3 was used as the test material, four treatment levels of low temperature and weak light were set, changes of leaf area, dry matter weight with different leaf ages were measured. The results showed that low temperature stress had a great effect on morphological development of leaves less than 15 days old, compared with the control, the decrease of leaf area was relatively large, but the effect on leaves aged 15-25 days was small; Under moderate low temperature (10-15 ℃) and weak light (photosynthetic photon flux density, PPFD 100-200 μmol·m^-2·s^-1), growth of leaf area decreased significantly and dry matter weight of leaves increased in the extension stage (5-15 d); The morphological development of leaves less than 15 days was basically stagnated under severe low temperature (5-10 ℃) and weak light (0-100 μmol·m^-2·s^-1), compared with the control, the decrease of leaf dry matter weight was relatively large; Mild low temperature and weak light stress (15-20 ℃, PPFD 200-400 μmol·m^-2·s^-1) did not significantly threaten the normal development of cucumber leaves, especially had little effect on the growth of leaf area and dry matter accumulation at the early stage of development. The results showed that in order to ensure the normal growth of leaves from the early stage of development to the expansion stage, moderate or above low temperature and weak light stress (5-15 ℃, PPFD 0-200 μmol·m^-2·s^-1) should be avoided.

Key words: cucumber, low temperature, weak light, leaf area, dry matter

中图分类号:

S642.2

唐卫东, 刘振文, 刘冬生, 胡雪华. 低温弱光胁迫对设施黄瓜叶片面积与干物质量的影响[J]. 浙江农业学报, 2022, 34(3): 517-524.

TANG Weidong, LIU Zhenwen, LIU Dongsheng, HU Xuehua. Effects of low temperature and weak light stress on leaf area and dry matter of cucumber in greenhouse[J]. Acta Agriculturae Zhejiangensis, 2022, 34(3): 517-524.

图/表 5

表1 正交试验设计

Table 1 Orthogonal design of experiment

试验号 Test number	温度 Temperature	光照 Light
1	1	1
2	1	2
3	1	3
4	1	4
5	2	1
6	2	2
7	2	3
8	2	4
9	3	1
10	3	2
11	3	3
12	3	4
13	4	1
14	4	2
15	4	3
16	4	4

图1 CK条件下弱光胁迫对不同叶龄叶片面积与干物质量的影响同处理各叶龄后不同小写字母表示显著差异(P<0.05),下同。

Fig.1 Effects of weak light stress on leaf area and dry matter of different leaf ages under CK Different lowercase letters behind the leaf ages in the same treatment indicated significant differences (P<0.05). The same as below.

图2 轻度低温处理下弱光胁迫对不同叶龄叶片面积与干物质量的影响

Fig.2 Effects of weak light stress on leaf area and dry matter of different leaf ages under mild low temperature

图3 中度低温处理下弱光胁迫对不同叶龄叶片面积与干物质量的影响

Fig.3 Effects of weak light stress on leaf area and dry matter of different leaf ages under moderate low temperature

图4 重度低温处理下弱光胁迫对不同叶龄叶片面积与干物质量的影响

Fig.4 Effects of weak light stress on leaf area and dry matter of different leaf ages under severe low temperature

参考文献 28

[1]	WRIGHT I J, REICH P B, WESTOBY M, et al. The worldwide leaf economics spectrum[J]. Nature, 2004, 428(6985): 821-827. DOI URL
[2]	ASHRAF M, HARRIS P J C. Photosynthesis under stressful environments: an overview[J]. Photosynthetica, 2013, 51(2): 163-190. DOI URL
[3]	薛立, 曹鹤. 逆境下植物叶性状变化的研究进展[J]. 生态环境学报, 2010, 19(8): 2004-2009.
	XUE L, CAO H. Changes of leaf traits of plants under stress resistance[J]. Ecology and Environmental Sciences, 2010, 19(8): 2004-2009. (in Chinese with English abstract)
[4]	高青海, 王亚坤, 陆晓民, 等. 低温弱光下外源褪黑素对黄瓜幼苗生长及抗氧化系统的影响[J]. 西北植物学报, 2014, 34(8): 1608-1613.
	GAO Q H, WANG Y K, LU X M, et al. Effects of exogenous melatonin on growth and antioxidant system of leaves in cucumber seedlings under low temperature and weak light stress[J]. Acta Botanica Boreali-Occidentalia Sinica, 2014, 34(8): 1608-1613. (in Chinese with English abstract)
[5]	赵春江, 陆声链, 郭新宇, 等. 数字植物研究进展: 植物形态结构三维数字化[J]. 中国农业科学, 2015, 48(17): 3415-3428.
	ZHAO C J, LU S L, GUO X Y, et al. Advances in research of digital plant: 3D digitization of plant morphological structure[J]. Scientia Agricultura Sinica, 2015, 48(17): 3415-3428. (in Chinese with English abstract)
[6]	唐卫东, 刘振文, 刘冬生, 等. 基于形态重构的叶片性状特征可视化表达方法[J]. 农业机械学报, 2019, 50(8): 249-256, 212.
	TANG W D, LIU Z W, LIU D S, et al. Visual expression method of leaf traits based on morphological reconstruction[J]. Transactions of the Chinese Society for Agricultural Machinery, 2019, 50(8): 249-256, 212. (in Chinese with English abstract)
[7]	熊宇, 杨再强, 薛晓萍, 等. 遮光处理对温室黄瓜幼龄植株叶片光合参数的影响[J]. 中国农业气象, 2016, 37(2): 222-230.
	XIONG Y, YANG Z Q, XUE X P, et al. Effect of shading on photosynthetic parameters in greenhouse cucumber leaves[J]. Chinese Journal of Agrometeorology, 2016, 37(2): 222-230. (in Chinese with English abstract)
[8]	PAN J S, TAN J Y, WANG Y H, et al. STAYGREEN (CsSGR) is a candidate for the anthracnose (Colletotrichum orbiculare) resistance locus cla in Gy14 cucumber[J]. Theoretical and Applied Genetics, 2018, 131(7): 1577-1587. DOI URL
[9]	MUÑOZ P, MUNNÉ-BOSCH S. Photo-oxidative stress during leaf, flower and fruit development[J]. Plant Physiology, 2018, 176(2): 1004-1014. DOI URL
[10]	陈春宏, 向邦银. 大型温室黄瓜生长发育特性研究[J]. 农业工程学报, 2005, 21(S2): 189-193.
	CHEN C H, XIANG B Y. Growth and development properties of cucumber under modern greenhouse cultivation[J]. Transactions of the Chinese Society of Agricultural Engineering, 2005, 21(S2): 189-193. (in Chinese with English abstract)
[11]	NIKOLAOU G, NEOCLEOUS D, CONSTANTINOS KITTAS N K. Modelling transpiration of soilless greenhouse cucumber and its relationship with leaf temperature in a Mediterranean climate[J]. Emirates Journal of Food and Agriculture, 2017, 29(12): 911.
[12]	SKUPIEŃ J, WÓJTOWICZ J, KOWALEWSKA Ł, et al. Dark-chilling induces substantial structural changes and modifies galactolipid and carotenoid composition during chloroplast biogenesis in cucumber (Cucumis sativus L.) cotyledons[J]. Plant Physiology and Biochemistry, 2017, 111: 107-118. DOI URL
[13]	李丹丹, 张丹, 张有利, 等. 弱光对黄瓜幼苗叶片光合产物积累的影响[J]. 黑龙江八一农垦大学学报, 2019, 31(1): 1-6.
	LI D D, ZHANG D, ZHANG Y L, et al. Effects of low-light stress on accumulation of photosynthetic product in leaves of cucumber seedlings[J]. Journal of Heilongjiang Bayi Agricultural University, 2019, 31(1): 1-6. (in Chinese with English abstract)
[14]	李伟, 眭晓蕾, 张振贤. 温度对黄瓜幼苗光合生理弱光耐受性的影响[J]. 应用生态学报, 2008, 19(12): 2643-2650.
	LI W, SUI X L, ZHANG Z X. Effects of temperature regime on low-light tolerance of Cucumis sativus seedling leaves in their photosynthesis[J]. Chinese Journal of Applied Ecology, 2008, 19(12): 2643-2650. (in Chinese with English abstract)
[15]	裴孝伯, 李世诚, 蔡润. 连续弱光处理对黄瓜生育及光合速率的影响[J]. 安徽农业大学学报, 2005, 32(3): 373-376.
	PEI X B, LI S C, CAI R. Effects of sustained low intensity light treatment on growth development and photosynthetic rate of cucumber[J]. Journal of Anhui Agricultural University, 2005, 32(3): 373-376. (in Chinese with English abstract)
[16]	李静, 李志军, 张富仓, 等. 水氮供应对温室黄瓜叶绿素含量及光合速率的影响[J]. 干旱地区农业研究, 2016, 34(5): 198-204.
	LI J, LI Z J, ZHANG F C, et al. Effects of water and nitrogen supply on chlorophyll content and photosynthetic rate of greenhouse cucumber[J]. Agricultural Research in the Arid Areas, 2016, 34(5): 198-204. (in Chinese with English abstract)
[17]	张中典, 张大龙, 李建明, 等. 黄瓜气孔导度、水力导度的环境响应及其调控蒸腾效应[J]. 农业机械学报, 2016, 47(6): 139-147.
	ZHANG Z D, ZHANG D L, LI J M, et al. Environmental response of stomatal and hydraulic conductances and their effects on regulating transpiration of cucumber[J]. Transactions of the Chinese Society for Agricultural Machinery, 2016, 47(6): 139-147. (in Chinese with English abstract)
[18]	BOZKURT S, KESKIN M. Effect of deficit irrigation on the estimation of cucumber leaf area under greenhouse conditions[J]. Journal of Agriculture and Biology, 2018, 20(4): 877-882.
[19]	YASUTAKE D, YU X, ASANO T, et al. Control of greenhouse humidity and airflow with fogging and circulation systems and its effect on leaf conductance in cucumber plants[J]. Environmental Control in Biology, 2014, 52(2): 101-105. DOI URL
[20]	SHANAZ Y, FARAHNAAZ R, SHUBANA B. Status and evaluation of systemic acquired resistance activators for the management of alternaria leaf spot of cucumber[J]. Journal of Advance Research in Science and Engineering, 2018, 7(4):2119-2132.
[21]	孙梅, 田昆, 张贇, 等. 植物叶片功能性状及其环境适应研究[J]. 植物科学学报, 2017, 35(6): 940-949.
	SUN M, TIAN K, ZHANG Y, et al. Research on leaf functional traits and their environmental adaptation[J]. Plant Science Journal, 2017, 35(6): 940-949. (in Chinese with English abstract)
[22]	张红梅, 余纪柱, 金海军. 低温弱光对黄瓜植株生长、光合特性的影响[J]. 沈阳农业大学学报, 2006, 37(3): 339-342.
	ZHANG H M, YU J Z, JIN H J. Effects of low temperature and poor light on growth, photosynthesis characters of cucumber[J]. Journal of Shenyang Agricultural University, 2006, 37(3): 339-342. (in Chinese with English abstract)
[23]	NOLAN T M, VUKAŠINOVIš N, LIU D R, et al. Brassinosteroids: multidimensional regulators of plant growth, development, and stress responses[J]. The Plant Cell, 2020, 32(2): 295-318.
[24]	DING H Y, ALI A, CHENG Z H. An allelopathic role for garlic root exudates in the regulation of carbohydrate metabolism in cucumber in a hydroponic co-culture system[J]. Plants, 2019, 9(1): 45. DOI URL
[25]	艾民, 刘振奎, 杨延杰, 等. 温度、光照强度和CO₂浓度对黄瓜叶片净光合速率的影响[J]. 沈阳农业大学学报, 2005, 36(4): 414-418.
	AI M, LIU Z K, YANG Y J, et al. Effects of temperature, light intensity and CO₂ concentration on cucumber net photosynthesis[J]. Journal of Shenyang Agricultural University, 2005, 36(4): 414-418. (in Chinese with English abstract)
[26]	SÄRKKÄ L E, JOKINEN K, OTTOSEN C O, et al. Effects of HPS and LED lighting on cucumber leaf photosynthesis, light quality penetration and temperature in the canopy, plant morphology and yield[J]. Agricultural and Food Science, 2017, 26(2): 101.
[27]	HUANG J Y, WANG C, LI X, et al. Conservation and divergence in the meiocyte sRNAomes of Arabidopsis, soybean, and cucumber[J]. Plant Physiology, 2020, 182(1): 301-317. DOI URL
[28]	WU H R, ZHU H J, HAN X, et al. Layout optimization for greenhouse WSN based on path loss analysis[J]. Computer Systems Science and Engineering, 2021, 37(1): 89-104. DOI URL

低温弱光胁迫对设施黄瓜叶片面积与干物质量的影响

Effects of low temperature and weak light stress on leaf area and dry matter of cucumber in greenhouse

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