低温弱光胁迫下辣椒苗期光合相关指标的杂种优势

doi:10.3969/j.issn.1004-1524.2021.03.07

浙江农业学报 ›› 2021, Vol. 33 ›› Issue (3): 429-436.DOI: 10.3969/j.issn.1004-1524.2021.03.07

低温弱光胁迫下辣椒苗期光合相关指标的杂种优势

苟秉调, 段盼盼, 杨楠, 赵淑芳, 王永富, 张高原, 魏兵强^*()

甘肃农业大学园艺学院,甘肃兰州 730070

收稿日期:2020-08-25 出版日期:2021-04-02 发布日期:2021-03-25
通讯作者: 魏兵强
作者简介:*, 魏兵强,E-mail: bqwei@gsau.edu.cn
苟秉调(1993—),女,甘肃定西人,硕士研究生,研究方向为蔬菜遗传与分子育种。E-mail: gsdiao@163.com
基金资助:
国家自然科学基金(31760572);甘肃农业大学青年导师扶持基金(GAU-QDFC-2020-07)

Heterosis analysis of photosynthetic parameters of pepper seedling responding to low temperature and low light stress

GOU Bingdiao, DUAN Panpan, YANG Nan, ZHAO Shufang, WANG Yongfu, ZHANG Gaoyuan, WEI Bingqiang^*()

College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China

Received:2020-08-25 Online:2021-04-02 Published:2021-03-25
Contact: WEI Bingqiang

摘要/Abstract

摘要：

为筛选可用于鉴定和预测辣椒杂种优势的光合指标,利用隶属函数值、灰色关联法和相关性分析法分析了低温弱光胁迫对辣椒亲本及其杂交种F₁苗期叶片净光合速率(P_n)、气孔导度(G_s)、胞间二氧化碳浓度(C_i)、蒸腾速率(T_r)和叶绿素含量的影响。结果表明:预测辣椒杂种优势的光合指标优劣顺序是C_i>G_s>叶绿素含量>T_r>P_n;低温弱光胁迫下,离中优势和超亲优势是评价辣椒杂种优势的优良度量标准; 3种辣椒材料的耐低温弱光能力大小为杂交种F₁>父本>母本。此研究丰富了辣椒杂种优势理论,为合理选用辣椒杂种优势鉴定标准、预测辣椒杂种优势提供了理论依据。

关键词: 辣椒, 低温弱光胁迫, 光合指标, 杂种优势

Abstract:

In order to screen the photosynthetic parameters for identifying and predicting the heterosis of pepper, using the parents and their F₁ hybrid of pepper as materials, effects of low temperature and low light on net photosynthetic rate (P_n), stomatal conductance (G_s), intercellular carbon dioxide concentration (C_i), transpiration rate (T_r) and chlorophyll content of pepper seedlings were comprehensively analyzed by the method of subordinate function values, grey correlation and correlation analysis. The results showed that the order of photosynthetic parameters to predict the heterosis of pepper from high to low was C_i, G_s, chlorophyll content, T_r and P_n. In addition, superiority from mid-parent heterosis and super-parent heterosis were chosen as the most suitable standards for appraising heterosis of pepper under low temperature and low light stress. The order of low temperature and low light resistance of the three materials was F₁ hybrid>male parent>female parent. This study enriched the heterosis theory of pepper, and provided theoretical basis for selecting photosynthetic parameters to predict the heterosis of pepper.

Key words: pepper, low temperature and low light stress, photosynthetic parameters, heterosis

中图分类号:

S641.3

苟秉调, 段盼盼, 杨楠, 赵淑芳, 王永富, 张高原, 魏兵强. 低温弱光胁迫下辣椒苗期光合相关指标的杂种优势[J]. 浙江农业学报, 2021, 33(3): 429-436.

GOU Bingdiao, DUAN Panpan, YANG Nan, ZHAO Shufang, WANG Yongfu, ZHANG Gaoyuan, WEI Bingqiang. Heterosis analysis of photosynthetic parameters of pepper seedling responding to low temperature and low light stress[J]. Acta Agriculturae Zhejiangensis, 2021, 33(3): 429-436.

图/表 9

表1 三种辣椒材料在低温弱光胁迫下的隶属函数值与各项光合指标值

Table 1 Subordinative function value and photosynthetic parameter values of three pepper accessions under low temperature and low light stress

样品 Sample	隶属函数值(X₀) Subordinative function value	P_n(X₁)/(μmol· m^-2·s^-1)	G_s (X₂)/(μmol· m^-2·s^-1)	C_i (X₃)/(μmol· mol^-1)	T_r(X₄)/(mmol· m^-2·s^-1)	C (X₅)
MP	0.724	5.003	0.035	326.629	1.682	41.433
FP	0.706	2.765	0.016	340.754	0.500	39.467
F₁	1.049	6.295	0.051	369.194	2.699	46.567

表2 不同辣椒材料在低温弱光胁迫下光合指标标准化处理的结果

Table 2 Standardized results of grey correlation analysis of photosynthetic parameter values of different pepper materials under low temperature and low light stress

样品 Sample	X₀'	X₁'	X₂'	X₃'	X₄'	X₅'
MP	0.724	0.916	0.663	-2.446	0.387	-0.589
FP	0.706	-93.064	-7.187	-1.145	-26.752	-7.832
F₁	1.049	1.594	4.325	3.378	7.541	8.174

表3 不同辣椒材料对低温弱光的抗性与各指标的关联系数、关联度和关联序

Table 3 Correlative modulus, correlative degree and correlative order between low temperature and low light resistance of different pepper materials and photosynthetic parameter values

样品 Sample	ξ₁	ξ₂	ξ₃	ξ₄	ξ₅
MP	1.000	0.996	0.933	1.001	0.989
FP	0.334	0.854	0.97	0.63	0.853
F₁	0.989	0.935	0.974	0.879	0.856
关联度	0.774	0.928	0.959	0.837	0.899
Correlative degree
关联序	5	2	1	4	3
Correlative order

图1 低温弱光胁迫对辣椒苗期叶片净光合速率及其杂种优势的影响 CK,对照;T1,低温弱光处理。柱状图上无相同小写字母的表示各处理间差异显著(P<0.05)。下同。

Fig.1 Effects of low temperature and low light stress on net photosynthetic rate and heterosis of pepper leaves at seeding stage CK, Control; T1, Low temperature and low light treatment. Data on the bars marked without the same lowercase letter indicated significant differences at P<0.05. The same as below.

图2 低温弱光胁迫对辣椒苗期叶片气孔导度及其杂种优势的影响

Fig.2 Effects of low temperature and low light stress on stomatal conductance and heterosis of pepper leaves at seeding stage

图3 低温弱光胁迫对辣椒苗期叶片胞间二氧化碳浓度及其杂种优势的影响

Fig.3 Effects of low temperature and low light stress on Ci and heterosis of pepper leaves at seeding stage

图4 低温弱光胁迫对辣椒苗期叶片蒸腾速率及其杂种优势的影响

Fig.4 Effects of low temperature and low light stress on transpiration rate and heterosis of pepper leaves at seeding stage

图5 低温弱光胁迫对辣椒苗期叶片叶绿素含量及其杂种优势的影响

Fig.5 Effects of low temperature and low light stress on chlorophyll content and heterosis of pepper leaves at seeding stage

表4 低温弱光胁迫下辣椒苗期光合相关指标与杂种优势的相关性分析

Table 4 Correlation analysis between photosynthetic relative indexes and heterosis of pepper at seedling stage under low temperature and low light stress

指标 Index	P_n	G_s	C_i	T_r	C	超中优势 Mid-parent heterosis	超亲优势 Super-parent heterosis
G_s	0.889^**
C_i	0.489	0.626
T_r	0.891^**	0.987^**	0.587
C	0.757^*	0.867^**	0.676^*	0.913^**
超中优势 Mid-parent heterosis	-0.355	-0.252	-0.006	-0.133	0.213
超亲优势 Super-parent heterosis	0.034	0.18	-0.071	0.251	0.466	0.866
离中优势 Superiority from mid-parent	0.707	0.573	-0.604	0.552	0.022	-0.113	-0.065

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低温弱光胁迫下辣椒苗期光合相关指标的杂种优势

Heterosis analysis of photosynthetic parameters of pepper seedling responding to low temperature and low light stress

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