家蚕品种‘W-1’己3期雌雄卵可溶性蛋白质表达谱研究

›› 2010, Vol. 22 ›› Issue (3): 0-307.

• 论文 •

家蚕品种‘W-1’己₃期雌雄卵可溶性蛋白质表达谱研究

谢再东^1,3,李兵^1,2,3,王举梅^1,3,许雅香^1,2,3,沈卫德^1,2,3,*

¹现代丝绸国家工程实验室,江苏苏州 215123；²苏州大学蚕桑研究所,江苏苏州 215123；³苏州大学基础医学与生物科学学院,江苏苏州 215123

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-05-25 发布日期:2010-05-25

Protein expression profiling of soluble protein of male and female eggs in the period of ji3 in Bombyx mori variety ‘W-1’

XIE Zai-dong;LI Bing;;WANG Ju-mei;XU Ya-xiang;;SHEN Wei-de;;*

¹The State Engineering Laboratory of Modern Silk, Suzhou 215123, China；²Institute of Sericulture, Soochow University, Suzhou 215123, China；³College of Basic Medicine and Biological Sciences, Soochow University, Suzhou 215123, China

Received:1900-01-01 Revised:1900-01-01 Online:2010-05-25 Published:2010-05-25

摘要/Abstract

摘要： 为探讨家蚕胚胎发育后期雌雄卵可溶性蛋白质组分的差异,用蛋白质双向电泳技术对家蚕卵色限性品种‘W-1’己3期蚕卵可溶性蛋白组成进行了比较分析。结果显示：雄卵(淡黄色)和雌卵(褐色)分别检测到317和335个斑点,能互相匹配的蛋白点273对,其中雄卵特异斑点44个,雌卵特异斑点62个。结果表明：雌雄蚕卵可溶性蛋白质组分存在差异。对特征蛋白进行了基质辅助质量飞行时间质谱(MALDI-TOF MS)分析,已鉴定的蛋白包括未知的角蛋白、原肌球蛋白同源体5、Sqd基因编码的RNA结合蛋白、转胶蛋白、热激蛋白20.4和原肌球蛋白1。进一步说明性别对胚胎器官和组织的形成有重要的影响。

关键词: 家蚕, 己₃, W-1, 可溶性蛋白质, 双向电泳

Abstract: To investigate the differential expression of soluble protein components, the soluble protein components of male and female eggs in the period of ji3 in Bombyx mori variety ‘W-1’ were compared by two-dimensional electrophoresis technology. The results showed that 317 and 335 protein spots were detected in soluble proteins of male and female egg respectively. Comparison analysis showed that 273 of the total protein spots were matched between male and female egg, and 44 and 62 specific protein spots were found in male and female egg respectively. The results indicated that soluble protein components in the period of ji3 in silkworm were different between the male and female. The characteristic protein spots were identified by MALDI-TOF MS. The result showed that proteins identified were putative keratin, tropomyosin isoform 5, RNA binding protein encoded by Sqd gene, transgelin, heat shock protein 20.4 and tropomyosin 1, indicating that gender has an important influence on the formation of embryonic organs and tissues．

Key words: Bombyx mori, Ji3, W-1, soluble protein, 2D-etectrophoresis

谢再东;李兵;王举梅;许雅香;沈卫德;*. 家蚕品种‘W-1’己₃期雌雄卵可溶性蛋白质表达谱研究[J]. , 2010, 22(3): 0-307.

XIE Zai-dong;LI Bing;WANG Ju-mei;XU Ya-xiang;SHEN Wei-de;*. Protein expression profiling of soluble protein of male and female eggs in the period of ji3 in Bombyx mori variety ‘W-1’[J]. , 2010, 22(3): 0-307.

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家蚕品种‘W-1’己₃期雌雄卵可溶性蛋白质表达谱研究

Protein expression profiling of soluble protein of male and female eggs in the period of ji3 in Bombyx mori variety ‘W-1’

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