关键词: ‘红阳’猕猴桃, 愈伤组织, 不定芽, 生根

Abstract: A stable and efficient rapid propagation system was established from the female Actinidia chinensis cv.Hongyang young leaves and stem segments with axillary buds in vitro. Leaves were cultured for the initiating of callus, proliferation of adventitious buds; and axillary buds were cultured for sprouting, multiplication and rooting. The results indicated that callus formation was easy and proliferation efficiency achieved 100% when explants from leaves were cultured on MS supplemented with 1.0 mg·L^-1 zeatin and 0.1 mg·L^-1 naphthylacetic acid (NAA). The average multiplication coefficient was 5.2,and the highest induction rate was 93.33%. The medium of MS supplemented with 1.0 mg·L^-1 6-benzylaminopurine (6-BA) and 0.1 mg·L^-1 NAA was optimal for sprouting of axillary bud,and the highest induction rate was 8333%. The multiplication coefficient of adventitious bud was 4.5 in the medium of MS supplemented with 2.0 mg·L^-1 6\|BA, 0.2 mg·L^-1 NAA and 0.1 mg·L^-1 gibberellic acid (GA). Moreover, the medium of 1/2 MS with 0.7 mg·L^-1 indolebutyric acid (IBA) was good for rooting.

Key words: Actinidia chinensis cv.Hongyang, callus, adventitious bud, rooting