Effects of different processed products of Terminalia chebula Retz. on 5-HT of small intestine in mice

doi:10.3969/j.issn.1004-1524.2016.10.06

›› 2016, Vol. 28 ›› Issue (10): 1663-1670.DOI: 10.3969/j.issn.1004-1524.2016.10.06

• Animal Science • Previous Articles Next Articles

Effects of different processed products of Terminalia chebula Retz. on 5-HT of small intestine in mice

ZHAO Si-qi^{1, 2}, HE Min^{1, 2, *}, LIANG Xiao-xia^{1, 2}, LI Xue^{1, 2}, LIAO Li^{1, 2}

1. College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China;
2. Key Laboratory of Animal Diseases and Human Health of Sichuan Province, Chengdu 611130, China

Received:2016-03-07 Online:2016-10-15 Published:2016-11-20

Abstract

Abstract: This study investigated the effects of Terminalia chebula Retz. and its 2 processed products of Tibetan medicine, Rubia cordifolia L. per Terminalia chebula and Euphorbia fischeriana Steud per Terminalia chebula on the secretory activity of 5-hydroxytryptamine immunoreactive (5-HT-IR) cells in small intestine of mice. Cellular localization and protein expression of 5-HT-IR cells in small intestine were revealed by immunohistochemistry. It was shown that 5-HT cells were widely distributed in small intestine, and the protein expression gradually reduced from duodenum to ileum. After drug delivery for 28 d, the protein expression of 5-HT of small intestine in mice was significantly lower in Rubia cordifolia L. per Terminalia chebula groups (in the dosage of 1.0 and 4.0 g·kg^-1), Euphorbia fischeriana Steud per Terminalia chebula groups (in the dosage of 1.0 and 4.0 g·kg^-1) and Terminalia chebula group (in the dosage of 4.0 g·kg^-1). The efficacy decreased as Rubia cordifolia L. per Terminalia chebula>Euphorbia fischeriana Steud per Terminalia chebula> Terminalia chebula. The efficacies of Terminalia chebula and its processed products in dosage of 4.0 g·kg^-1 were better than those in dosage of 1.0 g·kg^-1. The shape of 5-HT cells varied as cone, ellipse, and spindle. A large number of 5-HT-IR cells were found in the epithelial of intestinal gland and intestinal lamina propria in control group, while these cells were widely distributed in the epithelial cells of intestinal mucosal and epithelial of intestinal gland in experimental groups. This study indicated that Terminalia chebula and its processed products played an important role in adjusting physiological function of small intestine by inhibiting the secretion of 5-HT, and the effect of Rubia cordifolia L. per Terminalia chebula in the dosage of 4.0 g·kg^-1 was the best.

Key words: Terminalia chebula Retz., small intestine, 5-hydroxytryptamine(5-HT), immunohistochemistry

CLC Number:

S567.219

ZHAO Si-qi, HE Min, LIANG Xiao-xia, LI Xue, LIAO Li. Effects of different processed products of Terminalia chebula Retz. on 5-HT of small intestine in mice[J]. , 2016, 28(10): 1663-1670.

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Effects of different processed products of Terminalia chebula Retz. on 5-HT of small intestine in mice

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