Effects of fermentation metabolite produced by probiotics on intestinal morphology and intestinal barrier function in chicken

doi:10.3969/j.issn.1004-1524.2016.10.05

›› 2016, Vol. 28 ›› Issue (10): 1657-1662.DOI: 10.3969/j.issn.1004-1524.2016.10.05

• Animal Science • Previous Articles Next Articles

Effects of fermentation metabolite produced by probiotics on intestinal morphology and intestinal barrier function in chicken

ZHANG Jun¹, ZHU Jian-jin¹, LIU Jiang-ying¹, JIANG Rong², CHENG Xiang-rong¹

1. School of Food Science and Technology, Jiangnan University, Wuxi 214122, China;
2. Shanghai Sanzhi Biotechnology Co. Ltd., Shanghai 201706, China

Received:2016-02-19 Online:2016-10-15 Published:2016-11-20

Abstract

Abstract: In order to evaluate the effects of fermentation metabolite produced by probiotics on intestinal morphology and intestinal barrier function in chicken, 495 1-d-old broilers were randomly allotted into 5 dietary treatments, namely: Treatment Ⅰ, positive control, basal diet + bacitracin zinc 80 mg·kg^-1 feed + colistin sulfate 20 mg·kg^-1 feed + 2% unfermented feed; Treatment Ⅱ, negative control, basal diet + 2% unfermented feed; Treatment Ⅲ, basal diet + 1.0% fermented feed + 1% unfermented feed; Treatment Ⅳ, basal diet + 2.0% fermented feed; Treatment Ⅴ, basal diet + 2.0% sterilized fermented feed. The serum was collected for lipopolysaccharide (LPS) test, ileum was removed for observation of intestinal morphology by scanning electron microscopy (SEM), and the intestinal mucosa was scraped from the ileum by glass slide to determine the expression of intestinal tight junction. It was shown that the levels of serum lipopolysaccharide in treatment Ⅰ, Ⅳ, Ⅴ tended to be decreased significantly. The treatment Ⅴ and Ⅲ had higher and smoother intestinal villus in ileum than treatment Ⅱ, and the length of microvilli in these 2 treatments were significantly higher than that in treatment Ⅰ and Ⅳ, but villi density was less than that of treatment Ⅰ and Ⅳ. The expression of intestinal tight junction protein in treatment Ⅴ and Ⅳ were up-regulated significantly. These results suggested that not only probiotics but also fermentation metabolite played role in intestinal barrier function.

Key words: fermentation metabolite, broilers, intestinal morphology, tight junction protein

CLC Number:

S831

ZHANG Jun, ZHU Jian-jin, LIU Jiang-ying, JIANG Rong, CHENG Xiang-rong. Effects of fermentation metabolite produced by probiotics on intestinal morphology and intestinal barrier function in chicken[J]. , 2016, 28(10): 1657-1662.

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Effects of fermentation metabolite produced by probiotics on intestinal morphology and intestinal barrier function in chicken

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