Optimization of chlorophylls extraction from Cinnamomum camphora (L.) Presl leaf and its inhibitory effect against Staphylococcus aureus in liquid food

doi:10.3969/j.issn.1004-1524.2016.12.21

›› 2016, Vol. 28 ›› Issue (12): 2109-2115.DOI: 10.3969/j.issn.1004-1524.2016.12.21

• Food Science • Previous Articles Next Articles

Optimization of chlorophylls extraction from Cinnamomum camphora (L.) Presl leaf and its inhibitory effect against Staphylococcus aureus in liquid food

QUAN Qin-guo¹, DUAN Li-ping², HE Chu-xian¹, ZHOU Nong¹, LI Cheng-yong^1,*

1. College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China;
2. Department of Biochemistry, Hunan University of Science and Engineering, Yongzhou 425199, China

Received:2016-04-18 Online:2016-12-15 Published:2017-01-05

Abstract

Abstract: Chlorophyll was extracted from Cinnamomum camphora(L.) Presl leaves in Yongzhou via ultrasonic-assisted organic solvent, and the sterilization effect of the extracted chlorophylls against Staphylococcus aureus in liquid food was explored. It was shown that 4.667 mg·g^-1 chlorophyll could be obtained under the following optical conditions: solid/liquid ratio of 1: 20 g·mL^-1; ultrasonic treatment time of 8.8 min; extraction temperature of 50 ℃; extraction time of 3.9 h, and acetone-ethanol (2:1. V/V) as the extraction agent. The best inhibition effects of the extracted chlorophyll against S. aureus was achieved at the concentration of 10 mmol·L^-1 in NaCl-water system.

Key words: Cinnamomum camphora(L.) Presl, chlorophyll, liquid food, inhibitory effect

CLC Number:

R284.2

QUAN Qin-guo, DUAN Li-ping, HE Chu-xian, ZHOU Nong, LI Cheng-yong. Optimization of chlorophylls extraction from Cinnamomum camphora (L.) Presl leaf and its inhibitory effect against Staphylococcus aureus in liquid food[J]. , 2016, 28(12): 2109-2115.

References

[1] YEH R Y, SHIU Y L, SHEI S C, et al. Evaluation of the antibacterial activity of leaf and twig extracts of stout camphor tree, Cinnamomum kanehirae , and the effects on immunity and disease resistance of white shrimp, Litopenaeus vannamei [J]. Fish & Shellfish Immunology , 2008, 27(1):26-32.
[2] 陈铁壁, 全沁果, 段丽萍,等. 永州香樟叶精油的提取及其季节含量变化[J]. 天然产物研究与开发, 2016, 28(1):83-89.
CHEN T B, QUAN Q G, DUAN L P, et al. Essential oils extracting and content changes of Cinnamomum camphora (L.) leaf in Yongzhou[J]. Natural Product Research and Development , 2016, 28(1): 83-89. (in Chinese with English abstract)
[3] CORBETT B F, LEISER S C, LING H P, et al. Sodium channel cleavage is associated with aberrant neuronal activity and cognitive deficits in a mouse model of Alzheimer's disease[J]. Journal of Neuroscience , 2013, 33(16):7020-7026.
[4] 郑光耀, 何玲, 周涛涛,等. 松针叶绿素-胡萝卜素软膏对S180荷瘤小鼠的抗肿瘤作用及其对免疫功能的影响[J]. 肿瘤防治研究, 2012, 39(11):1324-1327.
ZHENG G Y, HE L, ZHOU T T,et al. Anti-tumor effects of chlorophyll-carotene paste from pine needles and immune function in sarcoma S180 tumor-bearing mice[J]. Cancer Research on Prevention and Treatment , 2012, 39(11): 1324-1327. (in Chinese with English abstract)
[5] CUMMINGS P L, KUO T, GASE L N, et al. Integrating sodium reduction strategies in the procurement process and contracting of food venues in the County of Los Angeles government, 2010-2012[J]. Journal of Public Health Management & Practice , 2014, 20(Supp. 1):S16-S22.
[6] 吴学文, 熊艳, 游奎一. 樟树叶挥发性成分研究[J]. 广西植物, 2011, 31(1):139-142.
WU X W, XIONG Y, YOU K Y. Essential oils from the leaves of Cinnamomum camphora [J]. Guihaia , 2011, 31(1): 139-142. (in Chinese with English abstract)
[7] 孙崇鲁. 香樟叶叶绿素的提取及稳定性研究[J]. 食品研究与开发, 2012, 33(11):61-64.
SUN C L. Extraction and stability of chlorophyll from Cinnamomum camphora leaves[J]. Food Research and Development , 2012, 33(11): 61-64. (in Chinese with English abstract)
[8] ARNON D I. Copper enzymes in isolated chloroplasts: Polyphenoloxidase in Beta vulgaris [J]. Plant Physiology , 1949, 24(1):1-15.
[9] 陈绍瑗, 吕贞儿, 董峰丽,等. 响应面分析法优化桑叶叶绿素提取工艺[J]. 浙江大学学报(农业与生命科学版), 2012, 38(6):725-731.
CHEN S Y, LYU Z E, DONG F L, et al. Optimization of chlorophyll extraction from mulberry leaves using response surface methodology[J]. Journal of Zhejiang University ( Agriculture & Life Sciences ), 2012, 38(6): 725-731.(in Chinese with English abstract)
[10] QIU X, SHARMA S, TUHELA L, et al. An integrated PEF pilot plant for continuous nonthermal pasteurization of fresh orange juice[J]. Transactions of the ASAE , 1998, 41(4):1069-1074.

[1]	SONG Xindan, CHEN Binbin, MA Zengling, XU Lili, LIN Lidong, WU Mingjiang. Effects of salinity level on photosynthetic characteristics of Sargassum fusiforme seedlings [J]. , 2020, 32(9): 1634-1644.
[2]	LIU Yiping, SU Shaowen, ZHANG Lin, LIU Ying, HUANG Zhiyuan, HE Dan, KONG Dezheng. Effect of exogenous calcium on lotus adaptation to salt stress [J]. , 2020, 32(2): 243-252.
[3]	SHI Zhaoyong, LI Ke, WANG Fayuan, WANG Xugang, XU Xiaofeng. Effects of nano-silver and exotic arbuscular mycorrhizal fungi on chlorophyll fluorescence kinetics of sweet sorghum [J]. , 2020, 32(2): 283-290.
[4]	WANG Nianyi, YU Fenghua, XU Tongyu, DU Wen, GUO Zhonghui, ZHANG Guosheng. Hyperspectral retrieval modelling for chlorophyll contents of japonica-rice leaves based on machine learning [J]. , 2020, 32(2): 359-366.
[5]	BIAN Jianwen, CUI Yan, YANG Songqi, LUO Guanghong, MENG Xiangang. Effects of Chlamydomonas debaryana Gor. and Anabaena azotica Ley. on wheat seedling growth under salt stress [J]. , 2020, 32(10): 1748-1756.
[6]	SUN Dezhi, YANG Hengshan, ZHANG Qingguo, FAN Fu, Suyale qiqige, PENG Jing, HAN Xiaori. Alleviating effect of exogenous nitric oxide donor sodium nitroprusside on tomato seedlings under salt stress [J]. , 2019, 31(8): 1286-1294.
[7]	FANG Fang, HE Xuchen, ZHANG Zhihao, ZHANG Qin, GUAN Yajing, HU Jin, HU Weimin. Response mechanism and stress resistance of maize inbred lines to high temperature stress at seedling stage [J]. , 2019, 31(7): 1045-1056.
[8]	LI Li, TIAN Shilin, JIANG Jun, SONG Li. Effects of nitric oxide on pepper growth and resistance indexes at seedling stage and fruit setting initial stage under weak light stress [J]. , 2019, 31(12): 2036-2042.
[9]	WU Degong, FANG Wenhao, DU Junli, YU Haibing, WANG Changjin, LI Jinpeng, CHU Lina, TANG Li. Effects of different aphid density stress on physiological substances in resistant and susceptible maize varieties at seedling stage [J]. , 2018, 30(4): 528-536.
[10]	ZHENG Xiang, JIANG Liangbo, DENG Bangliang, LIU Qian, LIU Xishuai, ZHENG Liya, GUO Xiaomin, LIU Yuanqiu, ZHANG Ling. Effects of enhanced UV-B radiation and nitrogen deposition on chlorophyll fluorescence parameters of invasive plant Triadica sebifera [J]. , 2018, 30(2): 248-254.
[11]	WU Yiting, YAO Chuanwei, DENG Boxia, ZHANG Xia, LI Shenghua, ZOU Juan, FU Ming. Identification of isolated fungus from root rot of Polygonatum sibiricum and its antagonistic endophytic bacteria [J]. , 2018, 30(12): 2087-2093.
[12]	ZHANG Ling, WANG Hua, ZHOU Jing, XU Qiang. Effects of NaCl stress on chlorophyll fluorescence characteristics and physiological characteristics in seedlings of two pepper cultivars [J]. , 2017, 29(4): 597-604.
[13]	YANG Fuqin, FENG Haikuan, LI Zhenhai, YANG Guijun, DAI Huayang. Estimation of apple leaf chlorophyll content based on hyperspectral data [J]. , 2017, 29(10): 1742-1748.
[14]	ZHANG Wanwan,YANG Keming， WANG Guoping， LIU Erxiong， LIU Cong. Study on GABP inversing modeling method of corn leaf chlorophyll content based on EMD and spectral derivative method [J]. , 2016, 28(8): 1297-.
[15]	GONG Shasha1， JIANG Hong1，2，*， MA Jinli1， SHU Haiyan3， CHEN Xiaofeng1. Variations of net ecosystem carbon exchange and chlorophyll fluorescence parameters of Phyllostachys edulis forest in Anji [J]. , 2016, 28(6): 1003-.

Optimization of chlorophylls extraction from Cinnamomum camphora (L.) Presl leaf and its inhibitory effect against Staphylococcus aureus in liquid food

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments