Phylogenetic relationships among 38 cultivars of Lagerstroemia indica based on ISSR molecular markers

doi:10.3969/j.issn.1004-1524.2019.04.08

›› 2019, Vol. 31 ›› Issue (4): 565-571.DOI: 10.3969/j.issn.1004-1524.2019.04.08

• Horticultural Science • Previous Articles Next Articles

Phylogenetic relationships among 38 cultivars of Lagerstroemia indica based on ISSR molecular markers

QIAO Zhongquan^1,2, WANG Xiaoming^1,2,*, LI Yongxin^1,2, ZENG Huijie^1,2, CAI Neng^1,2, LIU Sisi^1,2, CHEN Yi^1,2

1. Hunan Academy of Forestry, Changsha 410004, China;
2. Hunan Key Laboratory for Breeding of Clonally Propagated Forest Trees, Changsha 410004, China

Received:2018-09-20 Online:2019-04-25 Published:2019-04-19

Abstract

Abstract: Lagerstroemia indica is one of the world-famous ornamental plants in summer with lots of visitors, but the sources of most L. indica cultivars are poorly known, resulting in some difficulties in cultivars classification. This study analyzed the genetic relationships among 38 cultivars of L. indica by inter-simple sequence repeat (ISSR) molecular-marked technique, and screened 10 ISSR primers to assess the genetic relationship of 30 L. indica cultivars from America and 8 L. indica cultivars from China. The result showed that a total of 81 DNA bands were amplified and 70 of which (86.42%) were polymorphic. The genetic similar coefficient of 38 cultivars ranged from 0.543 2 to 0.988 7 with the average of 0.788 2, suggesting that the genetic relationships among these 38 cultivars were relatively close. When the genetic similarity of 0.736 was used as the threshold value, 38 cultivars were divided into three major groups by unweighted pair group method with arithmetic mean (UPGMA) method cluster analysis, and these cultivars could be divided into seven groups at similarity coefficient of 0.76. ISSR technique was efficient in generating accurate information of genetic diversity and relationship of L. indica cultivars.

Key words: Lagerstroemia indica, inter-simple sequence repeat, genetic relationship

CLC Number:

S436.661

QIAO Zhongquan, WANG Xiaoming, LI Yongxin, ZENG Huijie, CAI Neng, LIU Sisi, CHEN Yi. Phylogenetic relationships among 38 cultivars of Lagerstroemia indica based on ISSR molecular markers[J]. , 2019, 31(4): 565-571.

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Phylogenetic relationships among 38 cultivars of Lagerstroemia indica based on ISSR molecular markers

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