Effects of pH on physiological characters in somatic embryo induction stage of Agapanthus praecox

doi:10.3969/j.issn.1004-1524.2020.08.10

›› 2020, Vol. 32 ›› Issue (8): 1405-1414.DOI: 10.3969/j.issn.1004-1524.2020.08.10

• Horticultural Science • Previous Articles Next Articles

Effects of pH on physiological characters in somatic embryo induction stage of Agapanthus praecox

YUE Jianhua^1,2, DONG Yan³, LI Wenyang³, LI Meng^1,2, ZHANG Yan^1,2,*

1. College of Horticulture, Xinyang Agriculture and Forestry University, Xinyang 464100, China;
2. Key Laboratory of Horticultural Plants Genetic Improvement of Dabie Mountain, Xinyang 464100, China;
3. College of Forestry, Xinyang Agriculture and Forestry University, Xinyang 464100, China

Received:2020-01-09 Online:2020-08-25 Published:2020-08-28

Abstract

Abstract: Suitable medium pH maintains a suitable pH environment in the process of in vitro culture, which ensures the normal physiological metabolism of cells. To improve somatic embryogenesis efficiency in Agapanthus praecox, the medium pH parameter was optimized, and the regulatory effects of pH on plant hormones, carbon sources and oxidative stress during somatic embryogenesis were investigated. Pedicel originated embryogenic callus were used as materials, the induction efficiency of young somatic embryos (YSE) and mature somatic embryos (MSE) with gradient medium pH (5.6, 5.8, 6.0 and 6.2) in somatic embryo induction stage were revealed, respectively. Furthermore, physiological differences were detected by the ELISA technique, and correlation analyses among pH, embryo number and physiological indices were conducted. The results showed that the optimal pH values in YSE and MSE induction stages were 6.0 and 6.2, respectively. In YSE induction stage, content of endogenous IAA was positively correlated with YSE number (P<0.01), while content of GA₁ was negatively correlated with YSE number (P<0.05). In MSE induction stage, pH was positively correlated with MSE quantity, IAA oxidase activity, and sucrose content (P<0.05), respectively. Then IAA oxidase activity was positively correlated with MSE quantity (P<0.01), and the correlation between GA₄ content, sucrose content and MSE quantity were also strong. H₂O₂ content and YSE numbers showed a positive correlation, while the correlation between H₂O₂ and MSE numbers was negative. POD activity was negatively correlated with YSE numbers (P<0.05). pH in the process of YSE and MSE induction stages were optimized in this study, the results showed that endogenous IAA might be the crucial factor for somatic embryo induction under different pH conditions, GA₄ and sucrose contents played important roles in MSE induction.

Key words: Agapanthus praecox, pH, somatic embryogenesis, plant hormone, sugar metabolism, oxidative stress

CLC Number:

S682.1⁺9

YUE Jianhua, DONG Yan, LI Wenyang, LI Meng, ZHANG Yan. Effects of pH on physiological characters in somatic embryo induction stage of Agapanthus praecox[J]. , 2020, 32(8): 1405-1414.

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Effects of pH on physiological characters in somatic embryo induction stage of Agapanthus praecox

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