Isolation and phylogenetic analysis of Tremella sanguinea and its fungal host

doi:10.3969/j.issn.1004-1524.2021.03.09

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (3): 447-453.DOI: 10.3969/j.issn.1004-1524.2021.03.09

• Horticultrual Science • Previous Articles Next Articles

Isolation and phylogenetic analysis of Tremella sanguinea and its fungal host

CHEN Suo, HE Beibei, CHEN Qiwu, LI Li^*()

College of Life Science, Yangtze University, Jingzhou 434025, China

Received:2020-06-09 Online:2021-04-02 Published:2021-03-25
Contact: LI Li

Abstract

Abstract:

In order to study the taxonomic status of Tremella sanguinea Y.B. Peng and its fungal host, pure cultures, named as XE01 and XE02, were respectively obtained from basidiospores and basidiocarp tissues of Tremella sanguinea cultivated on Tilia sp. logs. The colony and microscopic morphology, as well as the molecular phylogeny based on rDNA-ITS of the two strains were then analyzed. It was found that the colonies of XE01 on PDA medium were white-creamy, smooth and moist; the cells were oval, budding and yeast-like. In the phylogenetic tree based on rDNA-ITS, XE01 was nested within in the clade of the genus Phaeotremella, and showed closer phylogenetic relationship to P. neofoliacea and P. eugeniae according to the genetic distances. The colonies of XE02 on PDA medium were fluffy and white; the hyphae were septate, and rarely branched; spherical spores and clamp connections were present; the inoculated agar medium was reddish brown due to the production of diffusible pigments. In the phylogenetic tree based on rDNA-ITS, XE02 was nested within the clade of the genus Stereum and showed closer phylogenetic relationship to S. rugosum according to the genetic distances. Based on these results, XE01 might be the free-living unicellular yeast state of T. sanguinea, and XE02 might be its fungal host. The results would contribute towards the classification of T. sanguinea and its fungal host, as well as the identification of their germplasm resources.

Key words: Tremella sanguinea, fungal host, morphology, molecular phylogeny

CLC Number:

S646

CHEN Suo, HE Beibei, CHEN Qiwu, LI Li. Isolation and phylogenetic analysis of Tremella sanguinea and its fungal host[J]. Acta Agriculturae Zhejiangensis, 2021, 33(3): 447-453.

Figures/Tables 6

References 26

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菌株 Strain	GenBank登录号 GenBank accession number
XE01	MN894289 (本研究This study)
XE02	MN894290 (本研究This study)
Phaeotremella foliacea DSM5493	MF580586^[16]
P. eugeniae LE303429	NR_158846^[16]
P. fimbriata Niemela 7897	MF076910^[16]
P. neofoliacea CBS8481	KY104488^[17]
P. skinneri CBS5029	NR_073211^[18]
P. frondosa LE206897	MF076907^[16]
P. mycophaga DSM104603	MF580589^[16]
P. pseudofoliacea CBS8227	KY104493^[17]
P. fagi CBS9964	NR_119558^[18]
P. mycetophiloides DSM5728	MF580587^[16]
Tremella globispora CBS6972	NR_155889^[19]
T. flava CBS8471	NR_155935^[17]
T. cinnabarina CBS8234	NR_155933^[17]
T.brasiliensis CBS8231	KY105675^[17]
T. fuciformis CBS6970	NR_155936^[17]
Stereum gausapatum CBS349.39	MH856041^[20]
S. sanguinolentum CBS529.50	MH856746^[20]
S. hirsutum FBCC1149	EU673087^[20]
S. armeniacum CBS944.96	MH862626^[20]
S. rugosum CBS108843	MH862811^[20]
Annulohypoxylon stygium XH1	FJ848850^[21]
香灰菌Hypoxylon sp.	N^[22]
Holtermannia corniformis CBS6979	AF410472^[8]
Rhizopus oryzae CBS112.07	AB097334^[23]

菌株 Strain	GenBank登录号 GenBank accession number
XE01	MN894289 (本研究This study)
XE02	MN894290 (本研究This study)
Phaeotremella foliacea DSM5493	MF580586^[16]
P. eugeniae LE303429	NR_158846^[16]
P. fimbriata Niemela 7897	MF076910^[16]
P. neofoliacea CBS8481	KY104488^[17]
P. skinneri CBS5029	NR_073211^[18]
P. frondosa LE206897	MF076907^[16]
P. mycophaga DSM104603	MF580589^[16]
P. pseudofoliacea CBS8227	KY104493^[17]
P. fagi CBS9964	NR_119558^[18]
P. mycetophiloides DSM5728	MF580587^[16]
Tremella globispora CBS6972	NR_155889^[19]
T. flava CBS8471	NR_155935^[17]
T. cinnabarina CBS8234	NR_155933^[17]
T.brasiliensis CBS8231	KY105675^[17]
T. fuciformis CBS6970	NR_155936^[17]
Stereum gausapatum CBS349.39	MH856041^[20]
S. sanguinolentum CBS529.50	MH856746^[20]
S. hirsutum FBCC1149	EU673087^[20]
S. armeniacum CBS944.96	MH862626^[20]
S. rugosum CBS108843	MH862811^[20]
Annulohypoxylon stygium XH1	FJ848850^[21]
香灰菌Hypoxylon sp.	N^[22]
Holtermannia corniformis CBS6979	AF410472^[8]
Rhizopus oryzae CBS112.07	AB097334^[23]

Isolation and phylogenetic analysis of Tremella sanguinea and its fungal host

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