Abstract:

In oder to clarify the phylogenetic relationship between Phyllagathis cavaleriei and its related species and provide data basis for its cross breeding,46 samples were collected from China, including P. cavaleriei and its related species P. cavaleriei var. tankahkeei and P. cavaleriei var. wilsoniana in China. Sequence of the internal transcribed spacer (ITS) of the ribosomal gene (nrDNA) of the tested samples was analyzed, and phylogenetic tree was constructed to study the phylogenetic relationship. The results showed that length of ITS1, ITS2, and 5.8S sequences of 46 materials were 246, 230, and 160-161 bp, respectively. Total 426 variable sites were found in ITS1 and ITS2 sequences with 232 informative sites. There were 202 mutation sites and 84 informative sites in 5.8S sequence. Based on ITS sequence, an evolutionary tree was constructed, materials could be divided into two branches. P. cavaleriei and P. cavaleriei var. tankahkeei were clustered into one branch in the cluster tree, and the branches were embedded into each other, while P. cavaleriei var. wilsoniana was clustered into a single branch. The above results showed that P. cavaleriei was closely related to P. cavaleriei var. tankahkeei, while P. cavaleriei var. wilsoniana was far related. The results supported Flora of China to deal with P. cavaleriei var. tankahkeei as a different name of P. cavaleriei, P. cavaleriei var. wilsoniana was a separate species.

Key words: Phyllagathis cavaleriei, Phyllagathis cavaleriei var. tankahkeei, Phyllagathis cavaleriei var. wilsoniana, internal transcribed spacer(ITS), phylogenetic relationship