Acta Agriculturae Zhejiangensis ›› 2022, Vol. 34 ›› Issue (6): 1316-1325.DOI: 10.3969/j.issn.1004-1524.2022.06.22

• Biosystms Engineening • Previous Articles     Next Articles

Effects of over expression of phosphopantetheinyl transferases gene on fatty acid synthesis in Synechocystis sp. PCC6803

LI Yanle1(), ZHONG Huairong2, XUAN Ning2, ZHANG Yan2, CHEN Gao2,*(), JI Xiang1,*()   

  1. 1. Inner Mongolia Key Laboratory of Biomass-Energy Conversion, School of Life and Technology, Inner Mongolia University of Science and Technology, Baotou 014010, Inner Mongolia, China
    2. Shandong Key Laboratory of Crop Genetics and Breeding and Ecological Physiology, Biotechnology Research Center, Shandong Academy of Agricultural Sciences, Jinan 250100, China
  • Received:2021-01-29 Online:2022-06-25 Published:2022-06-30
  • Contact: CHEN Gao,JI Xiang

Abstract:

Phosphopantothenyl transferase (PPTase) is a key enzyme in fatty acid synthesis in bacteria. In this study, the homologous recombinant plasmid slr0495(+) was successfully constructed for overexpressing the PPTase (slr0495) gene and transformed into wild-type Synechocystis sp. PCC6803. The results showed that under the culture conditions of 30 ℃, 50 μmol·m-2·s-1, the contents of C12:0, C16:0 and C18: 0 in wild type Synechocystis sp. were 1.06 mg·g-1, 6.44 mg·g-1, 1.19 mg·g-1,while the C12:0, C16:0and C18:0 contents of mutant strains were 23.58%, 25.31% and 13.45% higher than those of wild type strains, respectively. Under the culture conditions of 20 ℃, 50% NaNO3 and 50 μmol·m-2·s-1 light intensity, compared with the wild type, the contents of C16:0 and C18:0 in mutant strains overexpressing slr0495 gene increased by 44.71% and 41.51%, respectively. These above results indicated that overexpression of slr0495 gene increased the contents of saturated long-chain fatty acids in Synechocystis sp. PCC6803, and the contents of medium-long-chain saturated fatty acids were further improved under the conditions of low temperature (20 ℃) and nitrogen deficiency (50% NaNO3). This study not only provides a theoretical basis for further exploring the function of slr0495 and the stress response of this gene in adversity but also lays the foundation for further exploring the high-yield polyunsaturated fatty acids of microalgae.

Key words: Synechocystis sp. PCC6803, phosphopantetheinyl transferases, polyunsaturated fatty acids, homologous recombination

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