Population genetic structure of different resistance levels of Chilo suppressalis based on mitochondrial COⅠ and Cytb sequences in Zhejiang, China

doi:10.3969/j.issn.1004-1524.2022.11.15

Acta Agriculturae Zhejiangensis ›› 2022, Vol. 34 ›› Issue (11): 2462-2470.DOI: 10.3969/j.issn.1004-1524.2022.11.15

• Plant Protection • Previous Articles Next Articles

Population genetic structure of different resistance levels of Chilo suppressalis based on mitochondrial COⅠ and Cytb sequences in Zhejiang, China

LU Yanhui(), GUO Jiawen, TIAN Junce, XUE Zhaohong, ZHENG Xusong, LYU Zhongxian()

State Key Laboratory of Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2021-03-05 Online:2022-11-25 Published:2022-11-29
Contact: LYU Zhongxian

Abstract

Abstract:

In this study, genetic diversity and genetic structure of eight geographical populations with different resistance levels of Chilo suppressalis in Zhejiang Province were analyzed by using genetic methods based on mitochondrial cytochrome oxidase subunit Ⅰ (COⅠ) and cytochrome b (Cytb) genes. The analysis results of population genetic diversity showed that the COⅠ gene fragment with length of 627 bp and the Cytb gene fragment with length of 455 bp were obtained by PCR amplification and sequencing. Among them, 355 homologous COⅠ sequences detected 68 polymorphic sites, including 22 single mutation sites and 46 reduced mutation sites. A total of 85 haplotypes were defined, with an average of 18.25 haplotypes in each population, of which the Ruian (RA) population had the most haplotypes, with 27 haplotypes. Meanwhile, 326 Cytb homologous sequences detected 45 polymorphic sites, including 19 single mutation sites and 26 simple mutation sites. A total of 64 haplotypes were defined. The average haplotype of each population was 14.375, among which the most haplotypes were 25 in Yueqing (YQ) population. The highest haplotype diversity h of the population was 0.896 3 and 0.934 4, respectively. The analysis of population genetic structure showed that the majority of genetic variation existed among different geographical populations of C. suppressalis from individuals within the population, accounting for 80.30% (COⅠ gene) and 78.16% (Cytb gene). Only 19.43% (COⅠ gene) and 21.22% (Cytb gene) of the genetic differences were found among groups. In addition, no clear geographical differentiation was revealed in the haplotype network and on the neighbor-joining tree. Mantel correlation test showed that there was no significant correlation between genetic distance and geographical distance. These results provided basic data for different resistance level population exchange and control of C. suppressalis in Zhejiang Province.

Key words: Chilo suppressalis, mitochondrial cytochrome oxidase subunit Ⅰ, Cytb, genetic diversity, genetic structure, resistance

CLC Number:

S435.112.1

LU Yanhui, GUO Jiawen, TIAN Junce, XUE Zhaohong, ZHENG Xusong, LYU Zhongxian. Population genetic structure of different resistance levels of Chilo suppressalis based on mitochondrial COⅠ and Cytb sequences in Zhejiang, China[J]. Acta Agriculturae Zhejiangensis, 2022, 34(11): 2462-2470.

Figures/Tables 11

References 23

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种群 Popula- tions	采集地点 Collection locations	经纬度 Latitude and longitude	采集时间 Collection time
PH	平湖Pinghu	30°70'N, 121°02'E	2018年3月Mar. 2018
XiaoS	萧山Xiaoshan	30°17'N, 120°27'E	2018年4月Apr. 2018
XS	象山Xiangshan	29°48'N, 121°87'E	2018年4月Apr. 2018
NH	宁海Ninghai	29°28'N, 121°43'E	2018年3月Mar. 2018
YK	永康Yongkang	28°90'N, 120°03'E	2018年4月Apr. 2018
WL	温岭Wenling	28°37'N, 121°37'E	2018年4月Apr. 2018
YQ	乐清Yueqing	28°13'N, 120°95'E	2018年3月Mar. 2018
RA	瑞安Rui’an	27°78'N, 120°63'E	2018年4月Apr. 2018

种群 Popula- tions	采集地点 Collection locations	经纬度 Latitude and longitude	采集时间 Collection time
PH	平湖Pinghu	30°70'N, 121°02'E	2018年3月Mar. 2018
XiaoS	萧山Xiaoshan	30°17'N, 120°27'E	2018年4月Apr. 2018
XS	象山Xiangshan	29°48'N, 121°87'E	2018年4月Apr. 2018
NH	宁海Ninghai	29°28'N, 121°43'E	2018年3月Mar. 2018
YK	永康Yongkang	28°90'N, 120°03'E	2018年4月Apr. 2018
WL	温岭Wenling	28°37'N, 121°37'E	2018年4月Apr. 2018
YQ	乐清Yueqing	28°13'N, 120°95'E	2018年3月Mar. 2018
RA	瑞安Rui’an	27°78'N, 120°63'E	2018年4月Apr. 2018

基因名称 Gene name	引物 Primer sequence(5'-3')	产物长度 Product length/bp
COⅠ	F: TCTATGTGTGATGTTTATTG	700
	R: GATCAGGAATATTTGGGAAC
Cytb	F: TATGTACTACCATGAGGACAAATATC	490
	R: ATTACACCTCCTAATTTATTAGGAAT

基因名称 Gene name	引物 Primer sequence(5'-3')	产物长度 Product length/bp
COⅠ	F: TCTATGTGTGATGTTTATTG	700
	R: GATCAGGAATATTTGGGAAC
Cytb	F: TATGTACTACCATGAGGACAAATATC	490
	R: ATTACACCTCCTAATTTATTAGGAAT

药剂 Insecticides	种群 Populations	斜率±标准误 Slope±SE	卡方(自由度) χ² (df)	致死中浓度(95%置信限) LC₅₀ (95% FL)/(mg·L^-1)	抗性倍数 Resistance ratio
氯虫苯甲酰胺	PH	2.73±0.28	0.97(3)	80.58(67.16~98.32)	57.84
Chlorantraniliprole	XiaoS	2.11±0.36	0.87(3)	205.258(188.45~230.98)	147.35
	XS	2.87±0.41	3.93(3)	136.93 (118.09~162.22)	98.30
	NH	2.45±0.43	4.89(3)	178.403(155.42~210.04)	128.07
	YK	2.21±0.23	2.72(3)	196.68(171.15~215.16)	141.19
	WL	2.44±0.28	3.88(3)	301.96(256.53~347.25)	216.77
	YQ	2.59±0.26	5.67(3)	194.94(119.89~246.76)	139.94
	RA	1.75±0.21	4.07(3)	94.47(62.64~137.58)	67.82
阿维菌素	PH	2.51±0.39	2.60 (3)	17.84(15.37~21.98)	19.16
Abamectin	XiaoS	1.97±0.25	1.96(3)	13.92(11.36~17.42)	14.95
	XS	2.14±0.27	1.38(3)	9.11(7.32~12.04)	9.79
	NH	2.45±0.29	2.87(3)	13.53(11.70~16.39)	14.53
	YK	2.24±0.34	3.51(3)	12.88(9.52~18.06)	13.83
	WL	2.49±0.24	2.65(3)	8.57(6.38~10.83)	9.21
	YQ	2.61±0.36	1.94(3)	15.66(12.82~18.28)	16.82
	RA	2.28±0.25	1.83(3)	8.90(6.47~9.64)	9.56
甲氧虫酰肼	PH	2.11±0.28	2.32(3)	81.77(68.41~99.04)	112.17
Methoxyfenozide	XiaoS	1.74±0.21	2.15(3)	99.59(78.46~125.21)	136.61
	XS	2.35±0.25	1.89(3)	67.84(56.93~80.98)	93.06
	NH	2.74±0.27	1.54(3)	41.88(34.18~49.97)	57.45
	YK	2.23±0.24	3.09(3)	79.79(67.28~93.05)	109.45
	WL	2.43±0.21	2.92(3)	36.91(29.45~42.27)	50.47
	YQ	2.67±0.28	3.60(3)	73.13(58.94~91.63)	100.32
	RA	1.71±0.22	1.82(3)	88.76(69.63~112.35)	121.76

Population genetic structure of different resistance levels of Chilo suppressalis based on mitochondrial COⅠ and Cytb sequences in Zhejiang, China

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基因 Genes	种群 Populations	样本数量 N	单倍型数 H	多态位点数 s	单倍型多样度 h	平均核苷酸差异数 k	核苷酸多样性指数 π
COⅠ	PH	34	15	24	0.887 7	5.841 4	0.009 3
	XiaoS	46	13	28	0.861 8	5.963 3	0.009 5
	XS	34	20	28	0.930 5	5.260 3	0.008 5
	NH	49	9	15	0.511 1	1.821 4	0.002 9
	YK	49	15	20	0.682 0	3.649 7	0.005 9
	WL	46	19	22	0.858 9	4.637 7	0.007 4
	YQ	49	26	26	0.897 1	4.850 3	0.007 7
	RA	48	27	24	0.896 3	4.681 7	0.007 5
Cytb	PH	36	15	19	0.888 9	4.819 1	0.010 7
	XiaoS	40	9	16	0.669 2	3.152 6	0.006 9
	XS	32	16	19	0.907 3	4.300 4	0.009 5
	NH	49	6	12	0.266 2	1.821 4	0.003 4
	YK	49	18	17	0.651 4	3.331 6	0.007 3
	WL	52	17	21	0.865 0	4.070 1	0.009 0
	YQ	48	25	25	0.934 4	4.103 7	0.009 0
	RA	20	9	10	0.794 7	3.200 0	0.007 0

基因 Genes	种群 Populations	NH	PH	RA	WL	XiaoS	XS	YK	YQ
COⅠ	NH		0.009	0.009	0.008	0.008	0.008	0.005	0.009
	PH	0.354 39		0.010	0.009	0.011	0.010	0.008	0.009
	RA	0.451 48	0.112 08		0.008	0.011	0.008	0.008	0.008
	WL	0.334 68	0.089 83	0.015 68		0.010	0.008	0.007	0.008
	XiaoS	0.195 27	0.126 09	0.249 14	0.165 40		0.011	0.008	0.011
	XS	0.333 75	0.080 46	0.009 94	0.010 91	0.160 04		0.008	0.008
	YK	0.201 08	0.104 97	0.182 93	0.076 34	0.074 51	0.085 20		0.008
	YQ	0.407 02	0.086 50	0.011 48	0.007 13	0.217 52	-0.002 90	0.143 95
Cytb	NH		0.009	0.012	0.01	0.006	0.011	0.006	0.011
	PH	0.207 71		0.011	0.011	0.010	0.011	0.009	0.011
	RA	0.586 93	0.152 12		0.008	0.012	0.008	0.011	0.008
	WL	0.360 78	0.073 16	0.037 60		0.011	0.009	0.010	0.009
	XiaoS	0.075 21	0.131 28	0.439 96	0.276 78		0.012	0.008	0.012
	XS	0.418 74	0.065 35	0.005 43	0.004 03	0.320 59		0.010	0.009
	YK	0.062 62	0.042 35	0.309 33	0.153 98	0.065 49	0.181 29		0.010
	YQ	0.414 33	0.071 82	0.000 76	0.001 64	0.317 15	-0.015 99	0.193 50

基因 Genes	分组 Grouping	变异来源 Variation source	自由度 DF	方差总和 Variance sum	方差组分 Variance components	变异百分比 Variation percentage/%	F_st	P
COⅠ	(NH)&(PH)&(XiaoS)&	组间Between groups	4	144.863	0.550 85 Va	19.43	0.194 40	0.010 8
	(YK)&(RA,WL,XS,YQ)	组内群体间	3	7.828	0.007 58 Vb	0.27	0.003 30	0.338 2
		Intra population and inter group
		群体内个体间	347	789.757	2.275 96 Vc	80.30	0.197 00	0.000 1
		Between individuals within the population
		总和Total	354	942.448	2.834 39
Cytb	(NH)&(PH)&(XiaoS)&	组间Between groups	4	142.151	0.565 62 Va	21.22	0.212 20	0.014 06
	(YK)&(RA,WL,XS,YQ)	组内群体间	3	8.069	0.016 57 Vb	0.62	0.007 90	0.308 22
		Intra population and inter group
		群体内个体间	318	662.626	2.083 73 Vc	78.16	0.218 38	0.000 01
		Between individuals within the population
		总和Total	325	812.845	2.665 92

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药剂 Insecticides		单倍型多样度 h		核苷酸多样性指数 π
药剂 Insecticides		COⅠ	Cytb	COⅠ	Cytb
氯虫苯甲酰胺Chlorantraniliprole	R	-0.184	0.098	-0.198	-0.136
	P	0.663	0.818	0.638	0.748
阿维菌素Abamectin	R	-0.146	0.089	0.095	0.106
	P	0.730	0.835	0.823	0.803
甲氧虫酰肼Methoxyfenozide	R	0.431	0.270	0.604	0.207
	P	0.286	0.518	0.113	0.623

药剂 Insecticides		单倍型多样度 h		核苷酸多样性指数 π
药剂 Insecticides		COⅠ	Cytb	COⅠ	Cytb
氯虫苯甲酰胺Chlorantraniliprole	R	-0.184	0.098	-0.198	-0.136
	P	0.663	0.818	0.638	0.748
阿维菌素Abamectin	R	-0.146	0.089	0.095	0.106
	P	0.730	0.835	0.823	0.803
甲氧虫酰肼Methoxyfenozide	R	0.431	0.270	0.604	0.207
	P	0.286	0.518	0.113	0.623