Composition, classification and correlation analysis of bacteria in digestive tract of Opsariichthys bidens under sand filter mode

doi:10.3969/j.issn.1004-1524.20221283

Acta Agriculturae Zhejiangensis ›› 2023, Vol. 35 ›› Issue (10): 2286-2298.DOI: 10.3969/j.issn.1004-1524.20221283

• Animal Science • Previous Articles Next Articles

Composition, classification and correlation analysis of bacteria in digestive tract of Opsariichthys bidens under sand filter mode

HAN Mingming¹(), ZHAN Wei¹, HUANG Fuyong¹, LI Fangxing², LOU Bao¹^,^*()

1. Institude of Hydrobiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
2. Wuyi Xinyi Koi Family Farm, Wuyi 321200, Zhejiang, China

Received:2022-09-01 Online:2023-10-25 Published:2023-10-31

Abstract

Abstract:

Being an important stream fish, Opsariichthys bidens is widely distributed in all freshwater basins in China and occupies an important economic position.Microorganisms are closely related to the growth and health of fish. In order to improve the efficient and healthy aquaculture of O. bidens, the PCR amplification of the V3-V4 region of the total DNA in the digestive tract of O. bidens was performed to determine the composition, classification and correlations of the dominant bacteria. The results showed that among the dominant bacteria in the digestive tract of O. bidens, Bacillota, Bacteroidota, Fusobacteriota, Proteobacteria accounted for 79.32%, 4.75%, 2.55%, 1.69%, respectively. At the genus level, the relative abundance of Mycoplasma was the highest, which accounted for 77.50% of the total bacterial genera. The relative abundance of Mycoplasma was significantly (P<0.05) negatively correlated with Flavobacterium and Haliangium. Juged by the abunce of dominant bacteria in feed, aquaculture water and the surface of fliter sand, the main source of bacteria in the digestive tract was feed, which was followed by aquaculture water and filter sand. The sand filter played a certain role in filtering water, which basically met the requirements of healthy aquaculture of O. bidens in this system.

Key words: Opsariichthys bidens, microorganisms in digestive tract, dominant bacteria, sand filter system

CLC Number:

S917.1

HAN Mingming, ZHAN Wei, HUANG Fuyong, LI Fangxing, LOU Bao. Composition, classification and correlation analysis of bacteria in digestive tract of Opsariichthys bidens under sand filter mode[J]. Acta Agriculturae Zhejiangensis, 2023, 35(10): 2286-2298.

Figures/Tables 7

References 31

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属名 Genus	消化道中的相对丰度 Relative abundance in digestive tract	饲料中的相对丰度 Relative abundance in feed	水体中的相对丰度 Relative abundance in water	滤砂表面的相对丰度 Relative abundance on surface of filter sand
支原体属Mycoplasma	77.50	0.07	0.23	0.08
鲸杆菌属Cetobacterium	2.47	0	1.69	0.13
Muribaculaceae	1.97	7.95	1.50	1.08
普雷沃氏菌属Prevotella	1.35	14.15	1.00	0.56
拟杆菌属Bacteroides	1.10	2.95	0.59	1.44
奈瑟菌属Neisseria	0.89	10.39	1.22	0.29
链球菌属Streptococcus	0.64	7.07	0.57	0.14
乳杆菌属Lactobacillus	0.46	1.41	0.35	0.12
拟普雷沃菌属Alloprevotella	0.31	2.20	0.08	0.08
毛螺菌科NK4A136组	0.30	0.59	0.55	0.16
Lachnospiraceae_NK4A136_group
鞘氨醇单胞菌属Sphingomonas	0.30	0.70	0.16	0.29
粪杆菌属Faecalibacterium	0.29	1.07	0.62	0.08
埃希氏-志贺氏菌属Escherichia-Shigella	0.24	1.05	0.09	0.25
嗜血杆菌属Haemophilus	0.14	4.03	0.05	0.23
严格梭菌属Clostridium sensu_stricto_1	0.13	1.55	0.03	0.06
密螺旋体属Treponema	0.13	1.35	0.02	0.04
梭杆菌属Fusobacterium	0.08	2.89	0.05	0.09
硝化螺旋菌属Nitrospira	0.07	0.33	7.10	1.04
劳特普雷氏菌属Lautropia	0.07	1.10	0.05	0.17
Haliangium	0.04	0.13	0.97	1.62
放线菌属Actinomyces	0.03	1.54	0	0.01
新鞘氨醇菌属Novosphingobium	0.03	0.03	2.20	2.56
黄杆菌属Flavobacterium	0.02	1.03	2.55	2.00
红杆菌属Rhodobacter	0.01	0.02	3.27	1.29
SWB02	0.01	0.02	2.80	0.77

属名 Genus	消化道中的相对丰度 Relative abundance in digestive tract	饲料中的相对丰度 Relative abundance in feed	水体中的相对丰度 Relative abundance in water	滤砂表面的相对丰度 Relative abundance on surface of filter sand
支原体属Mycoplasma	77.50	0.07	0.23	0.08
鲸杆菌属Cetobacterium	2.47	0	1.69	0.13
Muribaculaceae	1.97	7.95	1.50	1.08
普雷沃氏菌属Prevotella	1.35	14.15	1.00	0.56
拟杆菌属Bacteroides	1.10	2.95	0.59	1.44
奈瑟菌属Neisseria	0.89	10.39	1.22	0.29
链球菌属Streptococcus	0.64	7.07	0.57	0.14
乳杆菌属Lactobacillus	0.46	1.41	0.35	0.12
拟普雷沃菌属Alloprevotella	0.31	2.20	0.08	0.08
毛螺菌科NK4A136组	0.30	0.59	0.55	0.16
Lachnospiraceae_NK4A136_group
鞘氨醇单胞菌属Sphingomonas	0.30	0.70	0.16	0.29
粪杆菌属Faecalibacterium	0.29	1.07	0.62	0.08
埃希氏-志贺氏菌属Escherichia-Shigella	0.24	1.05	0.09	0.25
嗜血杆菌属Haemophilus	0.14	4.03	0.05	0.23
严格梭菌属Clostridium sensu_stricto_1	0.13	1.55	0.03	0.06
密螺旋体属Treponema	0.13	1.35	0.02	0.04
梭杆菌属Fusobacterium	0.08	2.89	0.05	0.09
硝化螺旋菌属Nitrospira	0.07	0.33	7.10	1.04
劳特普雷氏菌属Lautropia	0.07	1.10	0.05	0.17
Haliangium	0.04	0.13	0.97	1.62
放线菌属Actinomyces	0.03	1.54	0	0.01
新鞘氨醇菌属Novosphingobium	0.03	0.03	2.20	2.56
黄杆菌属Flavobacterium	0.02	1.03	2.55	2.00
红杆菌属Rhodobacter	0.01	0.02	3.27	1.29
SWB02	0.01	0.02	2.80	0.77

Composition, classification and correlation analysis of bacteria in digestive tract of Opsariichthys bidens under sand filter mode

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