Effects of exogenous melatonin on active components content and antioxidase activity of Anoectochilus roxburghii

doi:10.3969/j.issn.1004-1524.2023.01.07

Acta Agriculturae Zhejiangensis ›› 2023, Vol. 35 ›› Issue (1): 58-66.DOI: 10.3969/j.issn.1004-1524.2023.01.07

• Horticultural Science • Previous Articles Next Articles

Effects of exogenous melatonin on active components content and antioxidase activity of Anoectochilus roxburghii

HE Xiuli(), WANG Renmin()

Seed Science and Technology Center, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China

Received:2021-09-06 Online:2023-01-25 Published:2023-02-21

Abstract

Abstract:

Anoectochilus roxburghii (Wall.) Lindl. is a rare perennial monocot herb in the family Orchidaceae, of important medicinal value. Melatonin (MT) is a class of amine hormones with various physiological functions such as regulating body day and night and antioxidant. In order to investigate the role of melatonin in the process of tissue culture seedling transplanting to the substrate, melatonin with 5 μmol·L^-1, 500 μmol·L^-1 and 5 mmol·L^-1 was used to study the effects of root irrigation on seedling growth and effective component content. The results showed that 5 μmol·L^-1 melatonin root irrigation treatment could significantly increase the total flavonoid by 27.27% and total amino acids content by 21.79%, whereas 500 μmol·L^-1 and 5 mmol·L^-1 melatonin significantly increased the polysaccharide content by 20.79% and 23.88%. Under the three concentrations of melatonin, the total chlorophyll content was 41.56%-55.84% significantly higher than that of the control. Under 5 μmol·L^-1 melatonin treatment, the activities of SOD, POD and CAT in leaves were significantly increased by 23.72%, 180.00% and 33.65%. In conclusion, melatonin could enhance the stress resistance of A.roxburghii by affecting chlorophyll content, metabolite accumulation and antioxidant enzyme activity, thus enhancing the vitality of A.roxburghii after transplanting.

Key words: Anoectochilus roxburghii, melatonin, active components, antioxidant enzyme, activity

CLC Number:

S682.31

HE Xiuli, WANG Renmin. Effects of exogenous melatonin on active components content and antioxidase activity of Anoectochilus roxburghii[J]. Acta Agriculturae Zhejiangensis, 2023, 35(1): 58-66.

Figures/Tables 10

References 32

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处理	株高	茎粗	叶长	叶宽	根长	根粗	单株鲜重	单株干重	折干率
Treatment	Plant height/cm	Stem diameter/mm	Leaf length/cm	Leaf width/cm	Root length/cm	Root diameter/mm	Fresh weight per plant/g	Dry weight per plant/g	Drying rate/%
CK	4.99±0.28 a	2.99±0.20 a	3.38±0.02 bc	2.73±0.05 a	3.40±0.35 a	1.18±0.07 b	1.17±0.14 a	0.11±0.01 a	9.59±1.50 a
M1	5.05±0.28 a	3.01±0.22 a	3.48±0.04 ab	2.81±0.07 a	3.40±0.19 a	1.37±0.02 a	1.18±0.03 a	0.11±0.01 a	9.52±0.23 a
M2	5.07±0.10 a	3.32±0.19 a	3.44±0.09 ab	2.75±0.04 a	2.81±0.15 ab	1.36±0.11 a	1.22±0.05 a	0.11±0.01 a	9.36±0.59 a
M3	5.08±0.18 a	3.09±0.36 a	3.29±0.04 c	2.65±0.13 a	2.69±0.18 b	1.26±0.06 ab	1.05±0.12 b	0.10±0.01 a	9.46±0.55 a

处理	株高	茎粗	叶长	叶宽	根长	根粗	单株鲜重	单株干重	折干率
Treatment	Plant height/cm	Stem diameter/mm	Leaf length/cm	Leaf width/cm	Root length/cm	Root diameter/mm	Fresh weight per plant/g	Dry weight per plant/g	Drying rate/%
CK	4.99±0.28 a	2.99±0.20 a	3.38±0.02 bc	2.73±0.05 a	3.40±0.35 a	1.18±0.07 b	1.17±0.14 a	0.11±0.01 a	9.59±1.50 a
M1	5.05±0.28 a	3.01±0.22 a	3.48±0.04 ab	2.81±0.07 a	3.40±0.19 a	1.37±0.02 a	1.18±0.03 a	0.11±0.01 a	9.52±0.23 a
M2	5.07±0.10 a	3.32±0.19 a	3.44±0.09 ab	2.75±0.04 a	2.81±0.15 ab	1.36±0.11 a	1.22±0.05 a	0.11±0.01 a	9.36±0.59 a
M3	5.08±0.18 a	3.09±0.36 a	3.29±0.04 c	2.65±0.13 a	2.69±0.18 b	1.26±0.06 ab	1.05±0.12 b	0.10±0.01 a	9.46±0.55 a

处理	Δ株高	Δ茎粗	Δ叶长	Δ叶宽	Δ根长	Δ根粗	Δ单株鲜重	Δ单株干重	Δ折干率
Treatment	ΔPlant height/cm	ΔStem diameter/mm	ΔLeaf length/cm	ΔLeaf width/cm	ΔRoot length/cm	ΔRoot diameter/mm	ΔFresh weight per plant/g	ΔDry weight per plant/g	ΔDrying rate/%
CK	0.66± 0.28 a	-0.08± 0.20a	0.25± 0.02 ab	0.27± 0.05 a	1.00± 0.35 a	-0.02± 0.07b	0.26± 0.14 a	0.03± 0.01 a	0.86± 1.50 a
M1	0.72± 0.28 a	-0.06± 0.22a	0.35± 0.04 a	0.35± 0.07 a	1.00± 0.19 a	0.17± 0.02 a	0.27± 0.03 a	0.03± 0.01 a	0.79± 0.24 a
M2	0.74± 0.10 a	0.25± 0.19 a	0.31± 0.09 a	0.29± 0.04 a	0.40± 0.15 b	0.16± 0.11 a	0.31± 0.05 a	0.04± 0.01 a	0.63± 0.59 a
M3	0.75± 0.18 a	0.02± 0.36 a	0.16± 0.04 b	0.19± 0.13 a	0.29± 0.18 b	0.06± 0.06ab	0.14± 0.12 a	0.02± 0.01 a	0.73± 0.55 a

处理	Δ株高	Δ茎粗	Δ叶长	Δ叶宽	Δ根长	Δ根粗	Δ单株鲜重	Δ单株干重	Δ折干率
Treatment	ΔPlant height/cm	ΔStem diameter/mm	ΔLeaf length/cm	ΔLeaf width/cm	ΔRoot length/cm	ΔRoot diameter/mm	ΔFresh weight per plant/g	ΔDry weight per plant/g	ΔDrying rate/%
CK	0.66± 0.28 a	-0.08± 0.20a	0.25± 0.02 ab	0.27± 0.05 a	1.00± 0.35 a	-0.02± 0.07b	0.26± 0.14 a	0.03± 0.01 a	0.86± 1.50 a
M1	0.72± 0.28 a	-0.06± 0.22a	0.35± 0.04 a	0.35± 0.07 a	1.00± 0.19 a	0.17± 0.02 a	0.27± 0.03 a	0.03± 0.01 a	0.79± 0.24 a
M2	0.74± 0.10 a	0.25± 0.19 a	0.31± 0.09 a	0.29± 0.04 a	0.40± 0.15 b	0.16± 0.11 a	0.31± 0.05 a	0.04± 0.01 a	0.63± 0.59 a
M3	0.75± 0.18 a	0.02± 0.36 a	0.16± 0.04 b	0.19± 0.13 a	0.29± 0.18 b	0.06± 0.06ab	0.14± 0.12 a	0.02± 0.01 a	0.73± 0.55 a

处理	Δ总黄酮含量	Δ多糖含量	Δ总生物碱含量	Δ总氨基酸含量
Treatment	ΔTotal content of flavonoid/%	ΔThe content of polysaccharide/%	ΔThe content of alkaloid/%	ΔTotal content of amino acid/%
CK	-0.28±0.01 b	-0.61±0.27 b	-0.29±0.08 a	4.01±0.44 b
M1	0.05±0.12 a	-0.40±0.08 b	-0.25±0.03 a	5.83±0.27 a
M2	-0.35±0.16 b	1.63±0.17 a	-0.17±0.22 a	3.32±0.14 c
M3	0.12±0.14 a	2.42±0.61 a	-0.25±0.11 a	1.96±0.02 d

Effects of exogenous melatonin on active components content and antioxidase activity of Anoectochilus roxburghii

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处理	Δ叶绿素a	Δ叶绿素b	Δ叶绿素a+b	Δ类胡萝卜素a
Treatment	ΔChlorophyll a	ΔChlorophyll b	ΔChlorophyll a+b	ΔCarotenoid
CK	0.14±0.09 b	-0.08±0.03 b	0.06±0.11 b	0.39±0.07 b
M1	0.37±0.04 a	0.01±0.04 a	0.38±0.08 a	0.54±0.05 a
M2	0.45±0.08 a	0.04±0.01 a	0.49±0.08 a	0.59±0.03 a
M3	0.37±0.12 a	0.00±0.05 a	0.38±0.17 a	0.53±0.08 ab

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