Biological characteristization of a new pest attacking Agrocybe aegerita: Bradysia peraffinis Tuomikoski 1960 (Diptera, Sciaridae)

doi:10.3969/j.issn.1004-1524.2023.04.15

Acta Agriculturae Zhejiangensis ›› 2023, Vol. 35 ›› Issue (4): 884-892.DOI: 10.3969/j.issn.1004-1524.2023.04.15

• Plant Protection • Previous Articles Next Articles

Biological characteristization of a new pest attacking Agrocybe aegerita: Bradysia peraffinis Tuomikoski 1960 (Diptera, Sciaridae)

WANG Yunping¹(), HE Peishan², MA Jiping¹, ZHONG Guoxiang¹, WANG Suzhen¹, XIAO Yinrun¹, XIONG Xiaowen¹, ZHANG Cheng¹^,^*()

1. Institute of Applied Agricultural Micro-Organisms,Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China
2. Jiangxi Agricultural Engineering College, Yichun 331200, Jiangxi, China

Received:2022-01-24 Online:2023-04-25 Published:2023-05-05

Abstract

Abstract:

In recent years, we discovered a species of insects that harmed the Agrocybe aegerita during its cultivation in Jiangxi Academy of Agricultural Sciences. Samples of adults were collected from the culture materials of Agrocybe aegerita, and molecular identification was carried out by amplifying the mitochondrial COⅠ gene and sequence comparison. The neighbor-joining method was used to construct phylogenetic trees. At the same time, we filmed and photographed the insect and described the main morphological characteristics of its eggs, larvae and adults. In addition, their biological characteristics were observed and recorded by raising them in an incubator. The results showed that the insect that harmed Agrocybe aegerita was Bradysia peraffinis Tuomikoski 1960, a new record species in Jiangxi, the similarity with Bradysia peraffinis COⅠ sequence (accession number: JQ613799) reached 99.85%. The larvae of this insect harm hyphae and fruit bodies. And we found the mature larvae crawled to the mouth of the culture bag and pupated, and flew out of the bag after eclosion. Adults were good at flying, with phototaxis. In laboratory tests, their generation period was about 20-29 days in the incubator. It had sevaral generations a year and the generations overlap. This article laid the foundation for the in-depth study of its occurrence and prevention and control techniques in the future.

Key words: Bradysia peraffinis, molecular identification, morphological characteristics, biology, Jiangxi

CLC Number:

S436.462

WANG Yunping, HE Peishan, MA Jiping, ZHONG Guoxiang, WANG Suzhen, XIAO Yinrun, XIONG Xiaowen, ZHANG Cheng. Biological characteristization of a new pest attacking Agrocybe aegerita: Bradysia peraffinis Tuomikoski 1960 (Diptera, Sciaridae)[J]. Acta Agriculturae Zhejiangensis, 2023, 35(4): 884-892.

Figures/Tables 10

References 18

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编号 Number	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15
1
2	0.002
3	0.003	0.002
4	0.191	0.189	0.191
5	0.179	0.182	0.184	0.176
6	0.191	0.189	0.191	0.156	0.162
7	0.179	0.177	0.179	0.164	0.188	0.148
8	0.184	0.181	0.184	0.160	0.175	0.177	0.148
9	0.162	0.160	0.162	0.156	0.183	0.145	0.156	0.176
10	0.189	0.187	0.189	0.166	0.200	0.142	0.176	0.160	0.174
11	0.191	0.189	0.191	0.188	0.199	0.175	0.177	0.201	0.194	0.174
12	0.198	0.196	0.198	0.156	0.190	0.146	0.142	0.162	0.164	0.156	0.162
13	0.168	0.166	0.168	0.142	0.162	0.158	0.162	0.158	0.164	0.146	0.169	0.158
14	0.195	0.193	0.195	0.208	0.191	0.199	0.195	0.201	0.176	0.216	0.199	0.183	0.190
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虫态 Stage	样本量 Number	体长 Body length/mm	平均体长 The average body length/mm
雌虫Female	108	2.08~3.02	2.54
雄虫Male	98	1.70~2.54	2.08
卵Egg	207	0.18~0.22	0.2
老熟幼虫Larva	153	5.03~5.54	5.43
蛹Pupa	92	2.01~2.74	2.29

虫态 Stage	样本量 Number	体长 Body length/mm	平均体长 The average body length/mm
雌虫Female	108	2.08~3.02	2.54
雄虫Male	98	1.70~2.54	2.08
卵Egg	207	0.18~0.22	0.2
老熟幼虫Larva	153	5.03~5.54	5.43
蛹Pupa	92	2.01~2.74	2.29

虫态 Stage	样本量 Number	历期 Duration/d	平均历期 The average duration/d
卵Egg	233	3~5	3.9
幼虫Larva	136	10~17	13.63
蛹Pupa	65	3~7	4.52
成虫Adult	109	1~5	3.86

Biological characteristization of a new pest attacking Agrocybe aegerita: Bradysia peraffinis Tuomikoski 1960 (Diptera, Sciaridae)

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