Design and testing of key components of tissue culture container cleaning devices

doi:10.3969/j.issn.1004-1524.20240351

Acta Agriculturae Zhejiangensis ›› 2025, Vol. 37 ›› Issue (2): 480-492.DOI: 10.3969/j.issn.1004-1524.20240351

• Biosystems Engineering • Previous Articles Next Articles

Design and testing of key components of tissue culture container cleaning devices

YU Guohong¹^,²(), ZHENG Hang¹^,², CHEN Bing¹, XUE Xianglei¹^,², XIE Ping³, YE Yunxiang¹^,²

1. Institute of Agricultural Equipment, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
2. Key Laboratory of Agricultural Equipment for Hilly and Mountainous Areas in Southeastern China(Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Hangzhou 310021, China
3. Changsha Jinggong Packaging Machinery Co., Ltd., Changsha 410006, China

Received:2024-04-12 Online:2025-02-25 Published:2025-03-20

Abstract

Abstract:

The industrialized production of tissue culture is the future development direction of seedling industry. At present, the tissue culture production in China is mainly carried out manually, and the production process includes a series of steps such as cleaning and filling of tissue culture containers, seed inoculation, cultivation, and transplantation. The cleaning of tissue culture containers is the key links for tissue culture industry. Manual cleaning is time-consuming laborious, with high operating costs and ineffective cleaning quality assurance. In this article, a small horizontal combination container cleaning equipment was designed on the basis of analyzing the requirements for cleaning tissue culture containers. The working principle of the bottle washing machine was emphasized, and the key components and control system modules were analyzed and designed. The orthogonal test was used to obtain the optimal combination of test conditions: the rotational speed of the scraper was 210 r·min^-1, the rotational time of the scraper was 2.5 s, the scraper in/out mode was forward-reverse rotation, the effect of grease clearing of the container in the histoculture reached more than 97%. Finally, a container cleaning test was conducted. The experimental results showed that the bottle washing machine ran smoothly, with an average cleaning qualification rate of over 98%, and the cleaning efficiency was 22 bottles per minute, which could meet the requirements of tissue culture factory production for bottle washing.

Key words: tissue culture container, industrialization, bottle washing machine, control system, test

CLC Number:

S225.92

YU Guohong, ZHENG Hang, CHEN Bing, XUE Xianglei, XIE Ping, YE Yunxiang. Design and testing of key components of tissue culture container cleaning devices[J]. Acta Agriculturae Zhejiangensis, 2025, 37(2): 480-492.

Figures/Tables 19

Fig.1 Structure diagram of tissue culture container cleaning production line

Fig.2 Schematic diagram of tissue culture container 1, Agar; 2, Bottle cap; 3, Bottle body.

Fig.3 Schematic diagram of the feeding mechanism structure 1. Rack; 2. Tissue culture containers; 3. Wheel 1; 4. Wheel 2; 5. Motor; 6. Synchronous belt; 7. Gears.

Fig.4 Dimensional drawing of feeding wheel

Fig.5 Schematic diagram of the flipping mechanism 1, Horizontal drum; 2, Tissue culture containers; 3, Driving motor.

Fig.6 Schematic diagram of degreasing mechanism structure 1, Lift the driving cylinder; 2, Guiding axis; 3, Bottom plate; 4, Driving cylinder for fixed blade component; 5, Installation plate for moving blade components; 6, Transmission bearing components; 7, Fixed scraper component; 8, Moving blade assembly; 9, Driving cylinder with the moving blade component; 10, Scraper blade rotation driving motor.

Fig.7 Schematic diagram of scraper mechanism structure 71, Scraper installation shaft; 72, Fixed scraper blade; 6, Transmission bearing components; 81, Moving scraper installation shaft; 82, Lift connecting rod 1; 83, Connecting rod 2; 84, Moving scraper blade; 111, Synchronous pulley.

Fig.8 Schematic diagram of the degreasing action process of the dynamic scraper mechanism

Fig.9 Schematic diagram of the motion of the moving scraper mechanism

Fig.10 Schematic diagram of the external washing components of the container 1, Rack; 2, Tissue culture containers; 41, External washing brush; 42, Driving motor.

Fig.11 Schematic diagram of the washing components inside the container 1, Brush; 2, Rack; 3, Feed cylinder; 4, Rotating driving motor; 5, Synchronous belt.

Fig.12 Pneumatic circuit

Fig.13 Flowchart of the culture medium cleaning control system

Fig.14 Grease cleaning test for tissue culture container

Table 1 Orthogonal test factors and levels

水平 Level	因素Factor
水平 Level	A刮片转速 Scraper speed/ (r·min^-1)	B旋转时间 Rotation time/s	C进出形式 Entry and exit forms
1	150	2.5	正转-正转
			Forward rotation- forward rotation
2	180	3.0	正转-停止
			Forward rotation-stop
3	210	3.5	正转-反转
			Forward rotation- reverse rotation

Table 2 Experimental results and analysis of variance

序号 No.	因素Factor				清脂率 Grease clearing rate(M)/%
序号 No.	A	B	C	空列Blank	清脂率 Grease clearing rate(M)/%
1	1	1	1	1	94.34
2	1	2	2	2	89.77
3	1	3	3	3	95.34
4	2	1	2	3	91.16
5	2	2	3	1	97.80
6	2	3	1	2	98.74
7	3	1	3	2	97.61
8	3	2	1	3	99.61
9	3	3	2	1	92.56
k₁	93.15	94.37	97.53	94.90	A₃
k₂	95.90	95.73	91.16	95.37	B₁
k₃	96.59	95.55	96.92	95.37	C₃
R	3.44	1.36	6.40	0.47
最优组合Optimal combination	A₃B₁C₃

Fig.15 Comparison of effects before and after grease cleaning for tissue culture container

Table 3 Validation test results of container cleaning rate

序号 No.	容器清洗合格率 Container cleaning qualification rate/%	容器清洗效率 Container cleaning efficiency/min^-1
1	98	22
2	100	22
3	98	22

Fig.16 Cleaning effect of tissue culture containers

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Design and testing of key components of tissue culture container cleaning devices

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