Effect of crude polysaccharides from Pleurotus eryngii on the mycelial growth of Pleurotus tuoliensis under high-temperature stress

doi:10.3969/j.issn.1004-1524.20250575

Acta Agriculturae Zhejiangensis ›› 2026, Vol. 38 ›› Issue (7): 1378-1387.DOI: 10.3969/j.issn.1004-1524.20250575

• Horticultural Science • Previous Articles Next Articles

Effect of crude polysaccharides from Pleurotus eryngii on the mycelial growth of Pleurotus tuoliensis under high-temperature stress

GAO Qingyun¹(), ZHANG Jing¹, XIONG Yichen¹, GUO Yuxiu¹, QIAN Lei¹, BAN Litong¹, LI Yiting¹^,^*(), QI Xin²^,^*()

¹ College of Agronomy and Resources Environment, Tianjin Agricultural University, Tianjin 300384, China
² Institute of Forestry and Fruit Tree Research, Tianjin Academy of Agricultural Sciences, Tianjin 300380, China

Received:2025-09-07 Online:2026-07-25 Published:2026-08-20

Abstract

Abstract:

Using Pleurotus eryngii fruiting bodies as raw material, crude polysaccharides were prepared via hot water extraction and fractionated alcohol precipitation, yielding three crude polysaccharide fractions, 56% PECP, 68% PECP, and 80% PECP. The in vitro antioxidant activity of these three PECP fractions, along with their effects on Pleurotus tuoliensis mycelial growth and mycelial recovery after high-temperature stress, were investigated. The results showed that all three PECP fractions exhibited excellent in vitro antioxidant activity, with 80% PECP demonstrating the highest activity. At a mass concentration of 10 mg·mL^-1, 80% PECP achieved a hydroxyl radical scavenging rate of 98.85% and a total reductive of 1.25. At a mass concentration of 2.5 mg·mL^-1, 80% PECP most significantly(p<0.05) promoted P. tuoliensis mycelial growth, with a growth rate of 5.37 mm·d^-1. Furthermore, adding 80% PECP accelerated the recovery of P. tuoliensis mycelia after high-temperature stress. Following 10 hours of 37 ℃ stress, mycelium treated with 80% PECP exhibited a recovery growth rate of 4.23 mm·d^-1. Simultaneously, compared with CK-HS, 80% PECP significantly reduced malondialdehyde (MDA) content by 18.55% and hydrogen peroxide (H₂O₂) content by 43.24% in P. tuoliensis mycelium after high-temperature stress, while significantly enhanced catalase (CAT) and peroxidase (POD) activities by 5.28% and 475.00%, respectively. Additionally, the protein and proline contents in mycelium treated with 80% PECP after high-temperature stress showed significant increased compared with CK-HS, rising by 9.44% and 16.55%, respectively. In summary, crude polysaccharides from P. eryngii exhibit excellent in vitro antioxidant activity and significantly promote mycelial growth, enhance antioxidant enzyme activity, and alleviate high-temperature damage in P. tuoliensis.

Key words: crude polysaccharides from Pleurotus eryngii, antioxidant activity, Pleurotus tuoliensis, mycelial growth, high-temperature stress

CLC Number:

S646

GAO Qingyun, ZHANG Jing, XIONG Yichen, GUO Yuxiu, QIAN Lei, BAN Litong, LI Yiting, QI Xin. Effect of crude polysaccharides from Pleurotus eryngii on the mycelial growth of Pleurotus tuoliensis under high-temperature stress[J]. Acta Agriculturae Zhejiangensis, 2026, 38(7): 1378-1387.

Figures/Tables 10

References 37

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组别 Group	质量浓度/ (mg·mL^-1) Mass concentration /(mg·mL^-1)	密度 Density	色泽 Color	萌发时间/h Germination time/h	满皿时间/d Full plate time/d	生长速度/ (mm·d^-1 Growth rate/ (mm·d^-1)
80% PECP	0.5	较浓密Relatively dense	浓白Dense white	12	7	3.12±0.47 cd
	1.0	较浓密Relatively dense	浓白Dense white	12	5	4.93±0.43 ab
	1.5	较浓密Relatively dense	浓白Dense white	14	6	3.85±0.23 c
	2.0	浓密Dense	浓白Dense white	10	6	4.18±0.33 bc
	2.5	浓密Dense	白色White	8	4	5.37±0.54 a
68% PECP	0.5	稀疏Sparse	白色White	14	6	4.02±0.50 bc
	1.0	较稀疏Relatively sparse	白色White	13	8	2.87±0.65 d
	1.5	浓密Dense	浓白Dense white	12	5	4.91±0.68 ab
	2.0	稀疏Sparse	白色White	12	6	3.85±0.03 c
	2.5	浓密Dense	白色White	10	4	5.31±0.16 a
56%	0.5	较浓密Relatively dense	浓白Dense white	16	7	3.22±0.34 cd
PECP	1.0	较稀疏Relatively sparse	浓白Dense white	16	8	2.52±0.95 d
	1.5	较浓密Relatively dense	浓白Dense white	16	7	3.50±0.28 cd
	2.0	较浓密Relatively dense	白色White	14	7	3.54±0.95 cd
	2.5	较浓密Relatively dense	浓白Dense white	12	5	4.52±0.34 ab
CK	—	较稀疏Relatively sparse	白色White	16	7	3.31±0.49 cd

组别 Group	质量浓度/ (mg·mL^-1) Mass concentration /(mg·mL^-1)	密度 Density	色泽 Color	萌发时间/h Germination time/h	满皿时间/d Full plate time/d	生长速度/ (mm·d^-1 Growth rate/ (mm·d^-1)
80% PECP	0.5	较浓密Relatively dense	浓白Dense white	12	7	3.12±0.47 cd
	1.0	较浓密Relatively dense	浓白Dense white	12	5	4.93±0.43 ab
	1.5	较浓密Relatively dense	浓白Dense white	14	6	3.85±0.23 c
	2.0	浓密Dense	浓白Dense white	10	6	4.18±0.33 bc
	2.5	浓密Dense	白色White	8	4	5.37±0.54 a
68% PECP	0.5	稀疏Sparse	白色White	14	6	4.02±0.50 bc
	1.0	较稀疏Relatively sparse	白色White	13	8	2.87±0.65 d
	1.5	浓密Dense	浓白Dense white	12	5	4.91±0.68 ab
	2.0	稀疏Sparse	白色White	12	6	3.85±0.03 c
	2.5	浓密Dense	白色White	10	4	5.31±0.16 a
56%	0.5	较浓密Relatively dense	浓白Dense white	16	7	3.22±0.34 cd
PECP	1.0	较稀疏Relatively sparse	浓白Dense white	16	8	2.52±0.95 d
	1.5	较浓密Relatively dense	浓白Dense white	16	7	3.50±0.28 cd
	2.0	较浓密Relatively dense	白色White	14	7	3.54±0.95 cd
	2.5	较浓密Relatively dense	浓白Dense white	12	5	4.52±0.34 ab
CK	—	较稀疏Relatively sparse	白色White	16	7	3.31±0.49 cd

组别 Group	胁迫时间/h Stress time/h	密度 Density	色泽 Color	满皿时间/d Full plate time/d	生长速度/(mm·d^-1) Growth rate/(mm·d^-1)
PECP-HS	2	浓密Dense	浓白Dense white	4	5.46±0.31 a
	4	浓密Dense	浓白Dense white	5	4.13±0.66 bc
	6	浓密Dense	浓白Dense white	5	4.39±0.63 b
	8	浓密Dense	浓白Dense white	7	3.38±0.26 cd
	10	较稀疏Relatively sparse	白色White	5	4.23±0.22 bc
CK-HS	2	浓密Dense	浓白Dense white	5	3.71±0.79 c
	4	较稀疏Relatively sparse	白色White	6	3.63±0.43 c
	6	浓密Dense	浓白Dense white	7	3.25±0.55 cd
	8	稀疏Sparse	白色White	7	3.00±0.13 d
	10	稀疏Sparse	白色White	8	2.82±0.29 d
CK	—	浓密Dense	浓白Dense white	6	3.61±0.82 c

组别 Group	胁迫时间/h Stress time/h	密度 Density	色泽 Color	满皿时间/d Full plate time/d	生长速度/(mm·d^-1) Growth rate/(mm·d^-1)
PECP-HS	2	浓密Dense	浓白Dense white	4	5.46±0.31 a
	4	浓密Dense	浓白Dense white	5	4.13±0.66 bc
	6	浓密Dense	浓白Dense white	5	4.39±0.63 b
	8	浓密Dense	浓白Dense white	7	3.38±0.26 cd
	10	较稀疏Relatively sparse	白色White	5	4.23±0.22 bc
CK-HS	2	浓密Dense	浓白Dense white	5	3.71±0.79 c
	4	较稀疏Relatively sparse	白色White	6	3.63±0.43 c
	6	浓密Dense	浓白Dense white	7	3.25±0.55 cd
	8	稀疏Sparse	白色White	7	3.00±0.13 d
	10	稀疏Sparse	白色White	8	2.82±0.29 d
CK	—	浓密Dense	浓白Dense white	6	3.61±0.82 c

Effect of crude polysaccharides from Pleurotus eryngii on the mycelial growth of Pleurotus tuoliensis under high-temperature stress

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组别 Group	用量Dosage
组别 Group	马铃薯 Potato	葡萄糖 Dextrose	琼脂 Agar	杏鲍菇粗多糖 PECP
CK	200	20	20	0
1	200	20	20	0.5
2	200	20	20	1.0
3	200	20	20	1.5
4	200	20	20	2.0
5	200	20	20	2.5

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