Effect of SlCHRC gene on tomato floral thermotolerance under high-temperature environment

doi:10.3969/j.issn.1004-1524.20250056

Acta Agriculturae Zhejiangensis ›› 2026, Vol. 38 ›› Issue (1): 67-75.DOI: 10.3969/j.issn.1004-1524.20250056

• Horticultural Science • Previous Articles Next Articles

Effect of SlCHRC gene on tomato floral thermotolerance under high-temperature environment

ZHU Changsong(), NA Qiting, ZHANG Mengzhuo, CAO Hui, LIU Shiying, ZHANG Zhengke, MENG Lanhuan^*()

School of Food Science and Engineering, Hainan University, Haikou 570228, China

Received:2025-01-20 Online:2026-01-25 Published:2026-02-11

Abstract

Abstract:

To investigate the role of the chromoplast-associated carotenoid-binding protein (CHRC) gene in the fibrillin (FBN) family of tomato (Solanum lycopersicum) in response to high-temperature stress, electrolyte leakage rate, malondialdehyde (MDA) content, antioxidant enzyme activities, ascorbic acid content, and the expression of heat-responsive genes were compared among flowers of wild-type tomato Micro Tom, SlCHRC gene-edited lines, and SlCHRC overexpression lines under high-temperature stress. The results showed that, compared with wild-type plants, under high-temperature stress, SlCHRC gene-edited lines exhibited a significant decrease in electrolyte leakage rate and MDA content, while the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), as well as ascorbic acid content, were significantly increased. Transcriptional levels of heat-responsive genes SlsHSP, SlHsfA2, SlHSP20, SlHSP70, and SlHSP90 were also significantly increased. In contrast, SlCHRC overexpression lines showed significantly reduced thermotolerance compared with wild-type plants. These results indicate that the tomato SlCHRC gene may be involved in regulating the response to high-temperature stress.

Key words: tomato, SlCHRC gene, CRISPR/Cas9, overexpression, flower, high-temperature stress

CLC Number:

S641.2

ZHU Changsong, NA Qiting, ZHANG Mengzhuo, CAO Hui, LIU Shiying, ZHANG Zhengke, MENG Lanhuan. Effect of SlCHRC gene on tomato floral thermotolerance under high-temperature environment[J]. Acta Agriculturae Zhejiangensis, 2026, 38(1): 67-75.

Figures/Tables 5

Table 1 Primers used for quantitative real time polymerase chain reaction

基因 Gene	基因编号 Gene code	正向引物序列(5'→3') Forward primer sequence(5'→3')	反向引物序列(5'→3') Reverse primer sequence (5'→3')
SlsHSP	Solyc11g020330	CTGGAAAGAGACGGCGAAGGG	CTCTCCGCTCACTCTCAACACTC
SlHSP20	Solyc09g015020	GGTGGTCGGAGGAGCAATATCTTC	CAGGGGCAGAGTATGGAGTGTTG
SlHSP70	Solyc04g009320	CAAGCTGAAAGAGCTCAAGG	CTGTCCCAGCTGCATTACTT
SlHSP90	Solyc05g010670	TCAGCAATTCTTCCGATGCTCT	TCCTTGGTTCCTGACCTTGC
SlHsfA2	Solyc08g062960	TTCCAGTTTCATTCGGCAGCTTAAC	CTCTGACCAACATTCCTCCTCCTC
ACTIN	Solyc03g078400	CAGCAGATGTGGATCTCAAA	CTGTGGACAATGGAAGGAC

Fig.1 Phenotypes, electrolyte leakage and malondialdehyde content of flowers in wild-type and SlCHRC gene-edited and SlCHRC overexpressing plants under high-temperature stress WT, Wild-type; CR-CHRC#12 and CR-CHRC#17, SlCHRC gene-edited lines; OE-CHRC#2 and OE-CHRC#6, SlCHRC gene overexpression lines; The bars marked without the same lowercase letter indicated significant differences at p<0.05. The same as below.

Fig.2 Flowering rate, fruit setting rate and flower drop rate of wild-type, SlCHRC gene-edited lines and overexpression lines after high-temperature treatment

Fig.3 Antioxidant enzyme activities and antioxidant substance contents in tomato flowers under high-temperature stress

Fig.4 Expression of heat-responsive related genes in tomato flowers under high-temperature stress

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Effect of SlCHRC gene on tomato floral thermotolerance under high-temperature environment

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