Abstract: Nicotiana tabacum geranylgeranyl diphosphate synthase gene （Ntggpps2） was a key enzyme gene for biosynthesis of the terpenoids and played important roles in plant metabolism and development. In order to study the function of Ntggpps2 in tobacco terpenoid synthesis， the plant overexpression vector of Ntggpps2 （pCAMBIA1302\|NT2） was constructed， and Ntggpps2\|overexpressing transgenic tobacco plants were obtained by Agrobacterium\|mediated genetic transformation. Quantitative RT\|PCR was conducted to quantify the transcript accumulation of Ntggpps2 in transgenic plants. The results clearly showed that the expression level of Ntggpps2 was increased by 2.2 fold in the leaves of transgenic line compared with wild type. The transgenic lines would provide a novel potential material and scientific basis for further biological function analysis of Ntggpps genes and molecular mechanism of terpenoids synthesis in tobacco.

Key words: terpenoids, geranylgeranyl diphosphate synthase, overexpression, genetic transformation