Isolation and culture of mesenchymal stem cells from duck embryo umbilical cord and its biological characteristics

Isolation and culture of mesenchymal stem cells from duck embryo umbilical cord and its biological characteristics

（Institute of Animal Science， Chinese Academy of Agricultural Sciences， Beijing 100193， China）

Online:2015-11-25 Published:2015-12-05

Abstract

Abstract: In order to provide experimental evidence for the clinical application of umbilical cord mesenchymal stem cells （UCMSCs） in the future， UCMSCs of duck were isolated in the present study by collagenase Ⅳ digesting method and were identified by adhesion molecules， such as CD29，CD44，CD71，CD73. A culture system of duck embryo umbilical cord derived mesenchymal stem cells was established. It was shown that UCMSCs isolated and cultured by this method could be differentiated into adipocytes and osteoblasts， which proved their multi-differentiation ability.

Key words: duck embryo, umbilical cord mesenchymal stem cells （UCMSCs）, biological characteristic, differentiation

WANG Wen\|jie， GUAN Wei\|jun*， MA Yue\|hui*， LI Xiang\|chen. Isolation and culture of mesenchymal stem cells from duck embryo umbilical cord and its biological characteristics[J]. .

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Isolation and culture of mesenchymal stem cells from duck embryo umbilical cord and its biological characteristics

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