Abstract: In order to transfer the clubroot resistance genes on A group chromosome of Brassica campestris L.ssp. Pekinensis（Lour.）Olsson to Brassica Oleracea and Brassica napus， radish cabbage cytoplasmic male sterility （CMS） lines C49\|141 with resistance to clubroot and cabbage inbred lines MWZS were used as female and male parents. The material of C49\|141“1/1”×MWZS“3/1” was successfully obtained on the culture medium of MS+100 mg·L-1 6\|BA+008 mg·L-1 NAA on 12 days after pollination by embryo rescue techniques. We used a series of methods to identify chromosome ploidy of plants， including micropropagation of plants，chromosome doubling， bacteria soil inoculation identification， as well as the morphological observation and identification of stomatal guard cell chloroplast number. Finally，We obtained the materials of Brassica napus ZZCZ13000， which was radish cytoplasmic male sterile and resistant to clubroot disease and had chromosome AACC; and interspecific hybridization materials ZZCZ13001， which had chromosome AC.

Key words: clubroot resistance, Brassica, radish CMS