Effect of growth-promoting bacteria on the degradation of thiamethoxam in Brassica rapa subsp. chinensis

doi:10.3969/j.issn.1004-1524.20240093

Acta Agriculturae Zhejiangensis ›› 2025, Vol. 37 ›› Issue (2): 394-404.DOI: 10.3969/j.issn.1004-1524.20240093

• Plant Protection • Previous Articles Next Articles

Effect of growth-promoting bacteria on the degradation of thiamethoxam in Brassica rapa subsp. chinensis

HU Rui¹^,²(), MA Liya², WAN Qun², WANG Ya², CAO Yaoyao², SHAO Sicheng¹, GE Jing², WU Xiangwei¹^,^*(), YU Xiangyang²^,^*()

1. College of Resources and Environment, Anhui Agricultural University, Hefei 230031, China
2. Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology, Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China

Received:2024-01-28 Online:2025-02-25 Published:2025-03-20
Contact: WU Xiangwei,YU Xiangyang

Abstract

Abstract:

To explore the impact of plant growth-promoting bacteria and their combined consortia on the degradation of thiamethoxam in Brassica rapa subsp. chinensis, this study used Pseudomonas sp. (NS6), Enterobacter sp. (NS54 and NS62), and Aeromonas hydrophila(NS69) as materials. Antagonistic tests were conducted to determine the compatibility between the strains, and based on plant growth-promoting characteristics experiments, the strains for constructing the composite microbial community were screened. Through pot experiments, the effects of single plant growth-promoting bacterium and the best-performing microbial consortium on the growth, quality, and thiamethoxam degradation in B. rapa subsp. chinensis were studied. The results showed that there was no antagonistic relationship among the strains, and the NS54+NS62+NS69 microbial consortium exhibited the best plant growth-promoting performance. The pot experiment results revealed that both the single strain treatments and the optimal microbial consortium treatment could improve the plant height, root length, fresh weight, dry weight, and chlorophyll content of B. rapa subsp. chinensis. They effectively reduced the malondialdehyde (MDA) content after thiamethoxam treatment and increased the contents of soluble sugars, soluble proteins, and vitamin C in B. rapa subsp. chinensis, with the microbial consortium treatment being superior to that of the single strain treatments. Additionally, the NS54+NS62+NS69 microbial consortium upregulated the expression of stress-responsive plant hormones (salicylic acid and jasmonic acid) related genes in B. rapa var. chinensis, thereby promoting the degradation of thiamethoxam. In summary, the NS54+NS62+NS69 microbial consortium not only promoted the growth and improved the quality of B. rapa subsp. Chinensis, but also effectively reduced the residual amount of thiamethoxam in the plant. This study result could provide an important theoretical foundation for ensuring the quality and safety of agricultural products and developing functional microbial agents

Key words: growth-promoting bacterium, microbial consortium, thiamethtoxam, Brassica rapa subsp. chinensis, growth-promotion, degradation

CLC Number:

S481.8

HU Rui, MA Liya, WAN Qun, WANG Ya, CAO Yaoyao, SHAO Sicheng, GE Jing, WU Xiangwei, YU Xiangyang. Effect of growth-promoting bacteria on the degradation of thiamethoxam in Brassica rapa subsp. chinensis[J]. Acta Agriculturae Zhejiangensis, 2025, 37(2): 394-404.

Figures/Tables 9

References 38

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基因 Gene	正向引物序列 Forward primer sequence(5'→3')	反向引物序列 Reverse primer sequence(5'→3')
BraA05g023030.3C	GGCTGGACCTACGCTATA	CGCAACACCTTCTTACGA
BraA03g024150.3C	ACCAATCCGCCATCTACT	TCACCGTCCACCATTCT
BraA03g042180.3C	TCGGAGGAACAAGTAGAGG	CCATCAGGCAACAGAACTC
BraA03g021140.3C	ACTTCCGCCTCGTCTTACTC	AGCCGCCTTGTCTGAGATAG
BraA09g044270.3C	CGCTATGGCTTCGTATTGC	GTTCTTCACCGTCTGTCTC
BraA05g036420.3C	CCTAAGCAGCCTCACTAAC	CACTCGGTCCACACTCTT
BraA07g016780.3C	GAGAGGAAGAGACTCATTGC	GCTCACCAACCGTAACTC
BraA07g028130.3C	GTTCTTCCGTGACCTTGAC	GAGTAGCAGCCAACATTGAG
BraA08g009510.3C	GGCTCTGGAATCATTGGAC	CCGACACAACCGCATTAG
BraA10g033710.3C	AGCGGAGGAGGCAATAGT	CGAATACGGCAGTGTCAAC
BraA05g007500.3C	ATGGAGAAGAGGCGATAGTC	CTGAACGAGTAAGGCAACG
BraA07g025310.3C	GGATGGTGGTGAGTGATG	CAAGTGGAGGCGATAGCA
BraA07g029730.3C	GCTCTGTAAGGTGGTGGA	GGCGTATGGATTGTTGGT
BraA04g019480.3C	CGTAGCCTCTTCTCTTCAG	CCTTAACCATCTCACCTCTC
BraA05g032610.3C	TCGGAGATTGGTCGGTGGT	ATGGAGAGCAGGATGAGTG

基因 Gene	正向引物序列 Forward primer sequence(5'→3')	反向引物序列 Reverse primer sequence(5'→3')
BraA05g023030.3C	GGCTGGACCTACGCTATA	CGCAACACCTTCTTACGA
BraA03g024150.3C	ACCAATCCGCCATCTACT	TCACCGTCCACCATTCT
BraA03g042180.3C	TCGGAGGAACAAGTAGAGG	CCATCAGGCAACAGAACTC
BraA03g021140.3C	ACTTCCGCCTCGTCTTACTC	AGCCGCCTTGTCTGAGATAG
BraA09g044270.3C	CGCTATGGCTTCGTATTGC	GTTCTTCACCGTCTGTCTC
BraA05g036420.3C	CCTAAGCAGCCTCACTAAC	CACTCGGTCCACACTCTT
BraA07g016780.3C	GAGAGGAAGAGACTCATTGC	GCTCACCAACCGTAACTC
BraA07g028130.3C	GTTCTTCCGTGACCTTGAC	GAGTAGCAGCCAACATTGAG
BraA08g009510.3C	GGCTCTGGAATCATTGGAC	CCGACACAACCGCATTAG
BraA10g033710.3C	AGCGGAGGAGGCAATAGT	CGAATACGGCAGTGTCAAC
BraA05g007500.3C	ATGGAGAAGAGGCGATAGTC	CTGAACGAGTAAGGCAACG
BraA07g025310.3C	GGATGGTGGTGAGTGATG	CAAGTGGAGGCGATAGCA
BraA07g029730.3C	GCTCTGTAAGGTGGTGGA	GGCGTATGGATTGTTGGT
BraA04g019480.3C	CGTAGCCTCTTCTCTTCAG	CCTTAACCATCTCACCTCTC
BraA05g032610.3C	TCGGAGATTGGTCGGTGGT	ATGGAGAGCAGGATGAGTG

组合编号 Combination number	产IAA量 IAA production/(mg·L^-1)	有效磷含量 Vailable phosphorus/(mg·L^-1)	铁载体活性 Iron carrier activity/%	固氮 Nitrogen fixation
NS6	83.16±0.01 a	133.9±0.1 j	5.16±0.14 k	-
NS54	71.09±0.10 bcd	167.3±2.1 e	22.73±0.79 h	-
NS62	70.96±0.08 bcd	170.1±1.0 d	25.69±0.73 f	-
NS69	59.37±0.05 e	53.3±0.1 m	41.40±0.31 a	+
C1:NS6+NS54	74.32±0.02 abc	155.7±0.3 h	18.42±0.45 j	-
C2:NS6+NS62	72.18±0.02 bcd	162.1±0.1 f	20.44±0.10 i	+
C3:NS6+NS69	69.41±0.03 bcd	94.3±0.1 l	19.64±0.28 ij	-
C4:NS54+NS62	79.10±0.05 a	183.9±0.2 a	31.35±0.49 c	-
C5:NS54+NS69	68.06±0.06 cde	151.0±0.6 h	26.42±0.32 e	+
C6:NS62+NS69	65.06±0.04 de	143.5±0.3 i	23.49±0.45 fg	+
C7:NS6+NS54+NS62	78.27±0.06 ab	174.5±0.5 c	28.52±0.29 d	-
C8:NS6+NS54+NS69	66.37±0.04 de	125.3±0.2 k	26.46±0.35 e	+
C9:NS54+NS62+NS69	76.43±0.05 abc	180.9±0.1 b	33.47±0.42 b	+
C10:NS6+NS54+NS62+NS69	75.29±0.01 abc	156.3±0.3 g	22.48±0.42 gh	+

组合编号 Combination number	产IAA量 IAA production/(mg·L^-1)	有效磷含量 Vailable phosphorus/(mg·L^-1)	铁载体活性 Iron carrier activity/%	固氮 Nitrogen fixation
NS6	83.16±0.01 a	133.9±0.1 j	5.16±0.14 k	-
NS54	71.09±0.10 bcd	167.3±2.1 e	22.73±0.79 h	-
NS62	70.96±0.08 bcd	170.1±1.0 d	25.69±0.73 f	-
NS69	59.37±0.05 e	53.3±0.1 m	41.40±0.31 a	+
C1:NS6+NS54	74.32±0.02 abc	155.7±0.3 h	18.42±0.45 j	-
C2:NS6+NS62	72.18±0.02 bcd	162.1±0.1 f	20.44±0.10 i	+
C3:NS6+NS69	69.41±0.03 bcd	94.3±0.1 l	19.64±0.28 ij	-
C4:NS54+NS62	79.10±0.05 a	183.9±0.2 a	31.35±0.49 c	-
C5:NS54+NS69	68.06±0.06 cde	151.0±0.6 h	26.42±0.32 e	+
C6:NS62+NS69	65.06±0.04 de	143.5±0.3 i	23.49±0.45 fg	+
C7:NS6+NS54+NS62	78.27±0.06 ab	174.5±0.5 c	28.52±0.29 d	-
C8:NS6+NS54+NS69	66.37±0.04 de	125.3±0.2 k	26.46±0.35 e	+
C9:NS54+NS62+NS69	76.43±0.05 abc	180.9±0.1 b	33.47±0.42 b	+
C10:NS6+NS54+NS62+NS69	75.29±0.01 abc	156.3±0.3 g	22.48±0.42 gh	+

处理 Treatment	丙二醛含量 MDA content/ (μmol·L^-1)	可溶性糖含量 Soluble sugar content/(g·kg^-1)	可溶性蛋白含量 Soluble protein content/(g·kg^-1)	维生素C含量 Vitamin C content/(mg·kg^-1)
TMX	3.86±0.25 a	9.35±0.51 c	11.17±0.73 c	79.3±6.3 c
NS54+TMX	2.78±0.15 bc	12.63±0.88 ab	13.57±0.30 a	106.6±9.3 ab
NS62+TMX	3.18±0.22 b	11.39±0.93 b	12.42±0.45 b	90.8±5.9 bc
NS69+TMX	2.65±0.42 cd	12.70±0.40 ab	13.03±0.37 ab	105.4±5.1 ab
C9+TMX	2.25±0.11 d	13.30±0.50 a	13.01±0.46 ab	113.7±6.1 a

Effect of growth-promoting bacteria on the degradation of thiamethoxam in Brassica rapa subsp. chinensis

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