Previous Articles     Next Articles

The expression analysis of GHR and IGF1 mRNA in embryonic skeletal muscle myoblasts of different duck breeds

  

  1. (Jiangsu Provincial Key Laboratory of Poultry Heredity & Breeding, Jiangsu Institute of Poultry Science, Yangzhou 225003, China)
  • Online:2016-03-25 Published:2016-04-07

Abstract: To investigate the expression patterns of growth hormone receptor (GHR) and insulinlike growth factor1 (IGF1) in duck skeletal muscle myoblasts, fluorescent realtime quantitative PCR was used to detect the distribution profile of GHR and IGFI mRNA in skeletal muscle myoblasts of Jinding and Gaoyou ducks at the embryonic day 13, 15, 17, 19, 21 and 23. The results showed that the expressions of IGF1 of Gaoyou ducks in breast and leg muscle myoblasts were significantly higher than that of Jinding ducks at embryonic day 13 (P<0.05); the embryonic day 17 was the common expression peak of GHR and IGF1 both in Gaoyou ducks and Jinding ducks; and then, significantly decreased. But at the embryonic day 19 and 21, the expressions of GHR and IGF1 in Gaoyou ducks breast muscle myoblasts were significantly higher than those of Jinding ducks (P<0.05), while there was no significant difference between varieties in the leg muscle myoblasts (P>0.05). The expression of GHR had a significantly linear positive correlation with the expression of IGF1 in breast and leg muscle myoblasts(P< 0.01). The results showed that the expression pattern of GHR was consistent with the expression of IGF1 between breeds; the expressions of GHR and IGF1 mRNA in the skeletal muscle myoblasts of duck embryo showed tissue specificity, and there might be a positive regulation mechanism between them.

Key words: duck, embryo, myoblasts, GHR, IGF1