›› 2017, Vol. 29 ›› Issue (9): 1445-1450.DOI: 10.3969/j.issn.1004-1524.2017.09.04

• Animal Science • Previous Articles     Next Articles

Antimicrobial resistance of Aeromonas hydrophila isolated from soft-shelled turtle Pelodiscus sinensis

ZHU Ningyu, ZHENG Xiaoye, CAO Feifei, LI Yangyang, ZHENG Tianlun*   

  1. Zhejiang Fisheries Technical Extension Center, Hangzhou 310023, China
  • Received:2017-02-16 Online:2017-09-20 Published:2017-09-27

Abstract: The antimicrobial resistance of 10 Aeromonas hydrophila strains collected from diseased soft-shelled turtle Pelodiscus sinensis was studied in this paper. Drug sensitive tests showed that all the 10 strains were sensitive to Amikacin, Neomycin, Cefoperazone, Aztreonam, Deoxytetracycline, Norfloxacin, Enrofloxacin and were risistent to Penicillin G, Lincomycin, Vancomycin, Sulfamethoxydiazine, but they showed different susceptibility to Florfenicol, Tetracycline, SMZ-TMP. At the same time, 17 antimicrobial resistant genes in the 10 strains were detected by PCR. The results showed that all these 10 strains carried 2-11 resistance genes. The highest detection rate of resistant gene qnrA and cat 1 reached 100% , while qepA, aph(3'), sul Ⅱ, sul Ⅲ, OXA were not detected. Different Aeromonas hydrophila strains showed different antimicrobial resistance, suggesting that it was necessary to carry out drug sensitivity tests before treating and preventing.

Key words: Pelodiscus sinensis, Aeromonas hydrophila, drug sensitive tests, antimicrobial resistant gene

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