Abstract: As one of the emerging contaminants, tetrabromobisphenol A (TBBPA) could accumulate in the soil and affect soil materials cycling as well as soil microbes. TBBPAs with different concentrations (4-40 mg·kg^-1) were added in soil to investigate their effects on $NH^{+}_{4}-N$, $NO^{-}_{2}-N$ and $NO^{-}_{3}-N$ transformation in lab. The bacterial community structures, as well as the prediction of the functions related to inorganic nitrogen transformation influenced by TBBPA, were analyzed with Illumina sequencing. It was shown that 40 mg·kg^-1 TBBPA had a significant (P<0.05) effect on soil inorganic nitrogen transformation. Addition of 40 mg·kg^-1 TBBPA increased the abundance of nitrifying bacteria and denitrifying bacteria and promoted $NH^{+}_{4}-N$ and $NO^{-}_{3}-N$ transformation in the aerobic condition, while decreased the abundance of denitrifying bacteria and inhibited $NO^{-}_{3}-N$ transformation in anaerobic condition. In addition, 40 mg·kg^-1 TBBPA also had remarkable effect on microbial community structure, including a positive effect on the relative abundances of Proteobacteria and a negative effect on the relative abundances of Chloroflexi and Actinobacteria. By predicting the microbial functions related to nitrogen transformation, it could be inferred that 40 mg·kg^-1 TBBPA induced the expression of narG and nirK and promoted $NO^{-}_{3}-N$ transformation in the aerobic condition, yet reduced the expression of nasB and inhibited $NO^{-}_{3}-N$ transformation in anaerobic condition.

Key words: soil microbes, inorganic nitrogen transformation, high-throughput sequencing, tetrabromobisphenol A