Characterization and fixation analysis of three atrazine-degrading strains

doi:10.3969/j.issn.1004-1524.2021.06.13

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (6): 1078-1087.DOI: 10.3969/j.issn.1004-1524.2021.06.13

• Environmental Science • Previous Articles Next Articles

Characterization and fixation analysis of three atrazine-degrading strains

LIU Dandan(), SUN Wanyu, WANG He

College of Environmental and Safety Engineering, Shenyang University of Chemical Technology, Shenyang 110142, China

Received:2020-12-03 Online:2021-06-25 Published:2021-06-25

Abstract

Abstract:

In this study, 3 strains with high-efficiency degradable bacteria were isolated from atrazine contaminated soil by soil dilution method and plate purification method, which were identified by morphologyical observation, physiological and biochemical analysis and 16S rDNA identification. The 3 strains were embeddedin four materials: sodium alginate, polyvinyl alcohol, gelatin, and biochar, and the physical properties of these fixed strains were compared after 24 hours. Meanwhile, the degradation rate and growth of the strains and immobilized strains were monitored by gas chromatography and spectrophotometry. The taxonomy of three degrading bacterias were found and identified by 16S rDNA sequencing and the phylogenetic tree constructed, respectively Shinella, Herbaspirillum, and Pseudomonas. All the strains reac-hed the maximum D₆₀₀ at 14 days and the degradation efficiency was higher than 85 percent. After comprehensively comparing the 4 kinds of fixing materials, sodium alginate is the most ideal material for embedding strains with its great formability, mechanical strength, and mass transfer performance. Besides, it has little effect on the degradation of atrazine. The microbial immobilization technology canimprove the toelrance of the strains which provides a theoretical basis for microbial remediation of conta-minated soil.

Key words: atrazine, atrazine-degrading strains, degradation rate, microbial immobilization

CLC Number:

S182
S154.3

LIU Dandan, SUN Wanyu, WANG He. Characterization and fixation analysis of three atrazine-degrading strains[J]. Acta Agriculturae Zhejiangensis, 2021, 33(6): 1078-1087.

Figures/Tables 10

Fig.1 Morphology of strain No. 8, 9 and 10 on LB medium

Fig.2 Morphology of 3 strains under scanning electron microscope (×20 000) A-C reprent No. 8-10 respectively. The picture shows the strain ×20 000.

Table 1 Physiological and biochemical identification results of 3 degrading bacteria

生理生化实验 Physiochemical test	8号 No. 8	9号 No. 9	10号 No. 10
革兰氏染色Gram staining	+	+	+
葡萄糖产酸Glucose produces acid	+	+	+
葡萄糖产气Glucose produce gas	-	-	-
蔗糖产酸Sucrose produces acid	+	+	+
蔗糖产气Sucrose produces gas	-	-	-
乳糖产酸Lactose produces acid	+	+	+
乳糖产气Lactose produces gas	-	-	-
淀粉水解Starch hydrolysis	+	+	+
接触酶Contact enzyme	+	+	+
柠檬酸盐利用Utilization of citrate	-	+	-
甲基红实验Methyl red experiment	-	-	-
V-P测定V-P determination	-	-	-
产氨Ammonia production	+	+	+
吲哚Indole	-	-	-

Fig.3 Phylogenetic tree based on 16S rDNA The accession number of the strain is shown in brackets.

Fig.6 Gas chromatography of atrazine degradation by strain No. 8-10 In the figure,A-C are gas chromatography of atrazine degradation by strain No. 8-10.

Fig.4 Degradation of atrazine by 3 strains In the figure, A-C are the degradation rates of atrazine by strain No. 8-10 on 5 days to 20 days,respectively.

Fig.5 The growth of the 3 strains from 5 days to 20 days

Table 2 Determination of physical properties of bacteria after embedding in different carriers

性状Trait	海藻酸钠Sodium alginate	聚乙烯醇Polyvinyl alcohol	明胶Gelatin	生物炭Biochar
机械强度Mechanical strength	0.82	0.80	0.60	0.93
传质能力Mass transfer performance	++	-	+	+
成形性 Formability	+	--	++	++

Fig.7 Forms of four immobilized bacterial strain agents A, Sodium alginate; B, Polyvinyl alcohol; C, Gelatin; D, Biochar.

Fig.8 Atrazine degradation rate of 3 strains immobilized on 4 carriers for 14 days

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Characterization and fixation analysis of three atrazine-degrading strains

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