Effects on photochemical fluorescence properties under salt-alkaline stresses about Sinocalycanthus chinensis

doi:10.3969/j.issn.1004-1524.2021.08.09

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (8): 1416-1425.DOI: 10.3969/j.issn.1004-1524.2021.08.09

• Horticultural Science • Previous Articles Next Articles

Effects on photochemical fluorescence properties under salt-alkaline stresses about Sinocalycanthus chinensis

ZHOU Beining(), MAO Lian, HUA Zhuangzhuang, LU Jianguo^*()

College of Landscape Architecture, Nanjing Forestry University, Nanjing 210037, China

Received:2020-12-15 Online:2021-08-25 Published:2021-08-27
Contact: LU Jianguo

Abstract

Abstract:

Three-year-old seedlings of Sinocalycanthus chinensis were used as experimental materials and set up eightalkaline salt NaHCO₃ treatment levels (CK, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%) to explore the effects of different alkaline salt stresses on the photosynthetic fluorescence characteristics of Sinocalycanthus chinensis, whichprovided theoretical guidance for the production practice and cultivation of Sinocalycanthus chinensis. The results showed that under NaHCO₃ stress, photosynthetic indices such as net photosynthetic rate (P_n), transpiration rate (T_r), stomatal conductance (G_s), water use efficiency (WUE), maximum net photosynthetic rate (P_nmax) and light saturation point (LSP) all showed downward trends, and the higher the concentration was, the more significant the decline. The intercellular CO₂ concentration (C_i) exhibited changes of decreasing followed by increase, while the light compensation point (LCP) showed an upward trend. With the increase of NaHCO₃ concentration, the OJIP curve of Sinocalycanthus chinensis leaves gradually showed an obvious K point, and F_o exhibited changes of decreasing followed by increase, while F_m, F_v, F_v/F_m and F_v/F_o exhibited changes of increasing followed by decrease. The light energy absorbed by the unit reaction center ABS/RC, the energy dissipated by heat DI_o/RC and the energy captured for Q_A reduction TR_o/RC all exhibited changes of decreasing followed by increase, while the energy used for electron transfer in the unit reaction center ET_o/RC gradually decreased. It indicated that the alkaline salt stress caused damage to the photosynthetic system of Sinocalycanthus chinensis, and the higher the concentration was, the greater the damage degree was. Low concentration of NaHCO₃ stress caused the decrease of net photosynthetic rate P_nof Sinocalycanthus chinensis due to stomatal limitation and had a weak inhibitory effect on the photochemical fluorescence performance of leaves, which properly improved the activity of PSⅡreaction center. The reason for the decrease of P_n caused by high concentration (>0.3%) stress might be non-stomatal restriction, seriously damaging the donor side and receptor side of PSⅡreaction center and leading to the inactivation of most PSⅡreaction centers.

Key words: Sinocalycanthus chinensis, saline-alkali stress, photosynthetic characteristic, chlorophyll fluorescence characteristic

CLC Number:

S685.17

ZHOU Beining, MAO Lian, HUA Zhuangzhuang, LU Jianguo. Effects on photochemical fluorescence properties under salt-alkaline stresses about Sinocalycanthus chinensis[J]. Acta Agriculturae Zhejiangensis, 2021, 33(8): 1416-1425.

Figures/Tables 9

References 30

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处理 Treatment/%	最大净光合速率 P_nmax/(μmol· m^-2·s^-1)	光饱和点 LSP/(μmol· m^-2·s^-1)	光补偿点 LCP/(μmol· m^-2·s^-1)	暗呼吸速率 R_d/(μmol· m^-2·s^-1)	表观量子效率 AQE/(mol· mol^-1)
CK	2.231 9±0.017 6 Aa	912.84±1.87 Aa	5.65±0.10 Eg	0.212 6±0.000 1 CcD	0.040 7±0.002 9 Aa
0.1	2.043 2±0.039 9 Bb	842.69±7.37 Bb	6.78±0.34 Ef	0.157 7±0.005 2 Ed	0.024 7±0.000 9 Bb
0.2	1.356 3±0.007 3 Cc	796.85±16.33 Cc	13.43±1.03 De	0.263 9±0.004 0 Aa	0.022 7±0.000 6 BbCc
0.3	0.991 6±0.000 5 Dd	797.73±14.46 Cc	13.94±0.44 De	0.231 1±0.005 3 BbC	0.019 8±0.000 7 BCcDd
0.4	0.940 4±0.010 9 De	658.89±7.91 Dd	16.94±1.22 Cd	0.242 2±0.005 5 Bb	0.017 1±0.000 1 Dd
0.5	0.303 4±0.002 1 Ef	578.94±3.58 Ee	17.93±0.61 Cc	0.070 1±0.006 2 Gf	0.004 5±0.000 2 Ee
0.6	0.196 9±0.000 3 Fg	446.95 ±0.73 Ff	19.20±0.73 Bb	0.201 1±0.005 4 cD	0.019 4±0.000 1 CcDd
0.7	0.160 5±0.006 4 Fg	430.98±4.28 Ff	21.34±0.08 Aa	0.092 7±0.001 6 Fe	0.006 2±0.000 5 Ee

处理 Treatment/%	最大净光合速率 P_nmax/(μmol· m^-2·s^-1)	光饱和点 LSP/(μmol· m^-2·s^-1)	光补偿点 LCP/(μmol· m^-2·s^-1)	暗呼吸速率 R_d/(μmol· m^-2·s^-1)	表观量子效率 AQE/(mol· mol^-1)
CK	2.231 9±0.017 6 Aa	912.84±1.87 Aa	5.65±0.10 Eg	0.212 6±0.000 1 CcD	0.040 7±0.002 9 Aa
0.1	2.043 2±0.039 9 Bb	842.69±7.37 Bb	6.78±0.34 Ef	0.157 7±0.005 2 Ed	0.024 7±0.000 9 Bb
0.2	1.356 3±0.007 3 Cc	796.85±16.33 Cc	13.43±1.03 De	0.263 9±0.004 0 Aa	0.022 7±0.000 6 BbCc
0.3	0.991 6±0.000 5 Dd	797.73±14.46 Cc	13.94±0.44 De	0.231 1±0.005 3 BbC	0.019 8±0.000 7 BCcDd
0.4	0.940 4±0.010 9 De	658.89±7.91 Dd	16.94±1.22 Cd	0.242 2±0.005 5 Bb	0.017 1±0.000 1 Dd
0.5	0.303 4±0.002 1 Ef	578.94±3.58 Ee	17.93±0.61 Cc	0.070 1±0.006 2 Gf	0.004 5±0.000 2 Ee
0.6	0.196 9±0.000 3 Fg	446.95 ±0.73 Ff	19.20±0.73 Bb	0.201 1±0.005 4 cD	0.019 4±0.000 1 CcDd
0.7	0.160 5±0.006 4 Fg	430.98±4.28 Ff	21.34±0.08 Aa	0.092 7±0.001 6 Fe	0.006 2±0.000 5 Ee

处理 Treatment/%	初始荧光 Initialfluorescence (F_o)	最大荧光 Maximum fluorescence (F_m)	可变荧光 Variable fluorescence (F_v)	最大光化学效率 Maximum photochemical efficiency(F_v/F_m)	潜在光化学效率 Potential photochemical efficiency(F_v/F_o)
CK	442.00±8.50 Bc	2 346.33±203.48 Aab	1 916.33±183.15 AaBb	0.815±0.008 Aab	4.32±0.34 Aab
0.1	439.33±7.54 Bc	2 489.00±44.47 Aab	2 036.33 ±31.75 AaB	0.818±0.006 Aab	4.64±0.10 Aab
0.2	423.00±5.86 Bc	2 391.33±32.05 Aab	1 980.33±21.53 AaBb	0.828±0.002 Aa	4.68±0.05 Aab
0.3	416.33±2.91 Bc	2 592.33±85.53 Aa	2 147.33±73.78 Aa	0.828±0.002 Aa	5.16±0.14 Aa
0.4	445.00±12.53 Bbc	2 522.67±88.38 Aab	2 083.33±79.74 AaB	0.826±0.003 Aab	4.69±0.24 Aab
0.5	470.00±3.00 Bbc	2 494.67±147.38 Aab	2 024.67±146.78 AaB	0.810±0.011 Aab	4.31±0.31 Aab
0.6	503.33±49.08 AaBb	2 376.00±105.13 Aab	1 872.67±145.33 AaBb	0.786±0.030 ABb	3.84±0.62 ABbc
0.7	552.00±10.97 Aa	2 189.00±45.21 Ab	1 637.00±56.15 Bb	0.747±0.010 Bc	2.97±0.16 Bc

处理 Treatment/%	初始荧光 Initialfluorescence (F_o)	最大荧光 Maximum fluorescence (F_m)	可变荧光 Variable fluorescence (F_v)	最大光化学效率 Maximum photochemical efficiency(F_v/F_m)	潜在光化学效率 Potential photochemical efficiency(F_v/F_o)
CK	442.00±8.50 Bc	2 346.33±203.48 Aab	1 916.33±183.15 AaBb	0.815±0.008 Aab	4.32±0.34 Aab
0.1	439.33±7.54 Bc	2 489.00±44.47 Aab	2 036.33 ±31.75 AaB	0.818±0.006 Aab	4.64±0.10 Aab
0.2	423.00±5.86 Bc	2 391.33±32.05 Aab	1 980.33±21.53 AaBb	0.828±0.002 Aa	4.68±0.05 Aab
0.3	416.33±2.91 Bc	2 592.33±85.53 Aa	2 147.33±73.78 Aa	0.828±0.002 Aa	5.16±0.14 Aa
0.4	445.00±12.53 Bbc	2 522.67±88.38 Aab	2 083.33±79.74 AaB	0.826±0.003 Aab	4.69±0.24 Aab
0.5	470.00±3.00 Bbc	2 494.67±147.38 Aab	2 024.67±146.78 AaB	0.810±0.011 Aab	4.31±0.31 Aab
0.6	503.33±49.08 AaBb	2 376.00±105.13 Aab	1 872.67±145.33 AaBb	0.786±0.030 ABb	3.84±0.62 ABbc
0.7	552.00±10.97 Aa	2 189.00±45.21 Ab	1 637.00±56.15 Bb	0.747±0.010 Bc	2.97±0.16 Bc

处理 Treatment/%	单位反应中心吸收的光能ABS/RC	单位反应中心热耗散的能量DI_o/RC	单位反应中心还原Q_A的能量TR_o/RC	单位反应中心电子传递的能量ET_o/RC
CK	2.81±0.22 Aab	0.52±0.06 Bbc	2.29±0.16 Aab	0.807±0.033 Aa
0.1	2.62±0.12 Ab	0.48±0.04 Bbc	2.15±0.08 Aab	0.783±0.005 Aa
0.2	2.67±0.13 Ab	0.46±0.03 Bbc	2.21±0.10 Aab	0.736±0.033 AaB
0.3	2.46±0.09 Ab	0.42±0.02 Bc	2.04±0.07 Ab	0.708±0.004 AaB
0.4	2.59±0.05 Ab	0.45±0.01 Bbc	2.14±0.04 Aab	0.718±0.017 AaB
0.5	2.71±0.19 Aab	0.52±0.07 Bbc	2.19±0.13 Aab	0.718±0.032 AaB
0.6	2.95±0.28 Aab	0.65±0.15 AaBb	2.30±0.12 Aab	0.681±0.057 AaB
0.7	3.24±0.17 Aa	0.82±0.01 Aa	2.43±0.16 Aa	0.496±0.133 Bb

Effects on photochemical fluorescence properties under salt-alkaline stresses about Sinocalycanthus chinensis

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