Effect of cryptochlorogenic acid on immunomodulatory and antioxidant in mice of lung injury induced by H9N2-AIV

doi:10.3969/j.issn.1004-1524.2022.01.05

Acta Agriculturae Zhejiangensis ›› 2022, Vol. 34 ›› Issue (1): 33-40.DOI: 10.3969/j.issn.1004-1524.2022.01.05

• Animal Science • Previous Articles Next Articles

Effect of cryptochlorogenic acid on immunomodulatory and antioxidant in mice of lung injury induced by H9N2-AIV

ZHANG Xin¹(), ZHANG Ming¹, HE Hongxuan²^,^*(), HU Yanchun¹^,^*()

1. College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
2. Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China

Received:2020-09-14 Online:2022-01-25 Published:2022-02-05
Contact: HE Hongxuan,HU Yanchun

Abstract

Abstract:

To investigate the effect of cryptochlorogenic acid(4-CQA)on lung injury and recovery in mice infected with avian influenza H9N2 virus, ninety SPF KM mice(6-8 weeks old)were randomly divided into H9N2 group,H9N2+4-CQA group, control group and 4-CQA group. Then H9N2-AIV was used to induce lung injury and received 4-CQA intragastric administration respectively in mice. The lung histopathlogy changes, the lung index and spleen index, and the contents of malondialdehyde (MDA),total superoxide dismutase (T-SOD) and myeloperoxidase (MPO) in the lung tissue homogenate, and the ratio of T cell subgroups (CD4⁺、CD8⁺、CD4⁺/CD8⁺) were observed. The results showed that the group of H9N2 presented depression and shortness of breath; lung index increased and spleen index decreased; histopathological manifestations of lung were alveolar atrophy, alveolar septal thickening and local lung consolidation; CD4⁺/CD8⁺ ratio of T lymphocyte subsets decreased significantly (P<0.05), activity of T-SOD decreased significantly, content of MDA increased significantly, and activity of MPO increased significantly (P<0.05). After 4-CQA treatment, lung index decreased and spleen index increased; lung histopathological damage was alleviated; CD4⁺/CD8⁺ ratio of T cell subgroups was significantly increased (P<0.05); T-SOD and MPO activity in lung were significantly increased and the content of MDA was significantly decreased (P<0.05). It was concluded that 4-CQA can alleviate the lung injury induced by avian influenza H9N2 virus by maintaining immune balance and reducing oxidative damage.

Key words: H9N2-AIV, 4-CQA, lung injury, immunomodulation, antioxidant

CLC Number:

S855.3

ZHANG Xin, ZHANG Ming, HE Hongxuan, HU Yanchun. Effect of cryptochlorogenic acid on immunomodulatory and antioxidant in mice of lung injury induced by H9N2-AIV[J]. Acta Agriculturae Zhejiangensis, 2022, 34(1): 33-40.

Figures/Tables 6

References 33

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组别 Group	第3天 3rd day		第5天 5th day		第7天7th day
组别 Group	肺指数 Lung index	脾指数 Spleen index	肺指数 Lung index	脾指数 Spleen index	肺指数 Lung index	脾指数 Spleen index
H9N2	0.80±0.04 a	0.30±0.02 a	0.89±0.02 a	0.33±0.01 c	1.03±0.01 a	0.38±0.01 b
H9N2+4-CQA	0.76±0.05 a	0.32±0.03 a	0.79±0.03 b	0.36±0.01 b	0.80±0.02 b	0.44±0.04 ab
对照组Control	0.66±0.03 b	0.35±0.04 a	0.65±0.05 c	0.41±0.01 a	0.66±0.05 c	0.47±0.03 a
4-CQA	0.68±0.02 b	0.35±0.04 a	0.68±0.04 c	0.41±0.01 a	0.64±0.03 c	0.47±0.03 a

组别 Group	第3天 3rd day		第5天 5th day		第7天7th day
组别 Group	肺指数 Lung index	脾指数 Spleen index	肺指数 Lung index	脾指数 Spleen index	肺指数 Lung index	脾指数 Spleen index
H9N2	0.80±0.04 a	0.30±0.02 a	0.89±0.02 a	0.33±0.01 c	1.03±0.01 a	0.38±0.01 b
H9N2+4-CQA	0.76±0.05 a	0.32±0.03 a	0.79±0.03 b	0.36±0.01 b	0.80±0.02 b	0.44±0.04 ab
对照组Control	0.66±0.03 b	0.35±0.04 a	0.65±0.05 c	0.41±0.01 a	0.66±0.05 c	0.47±0.03 a
4-CQA	0.68±0.02 b	0.35±0.04 a	0.68±0.04 c	0.41±0.01 a	0.64±0.03 c	0.47±0.03 a

组别Group	CD4⁺/%	CD8⁺/%	CD4⁺/CD8⁺
H9N2	17.61±3.92 a	10.37±2.61 a	1.71±0.06 b
H9N2+4-CQA	19.20±3.73 a	8.75±2.03 a	2.21±0.08 a
对照组Control	19.74±4.77 a	9.52±2.16 a	2.07±0.03 a
4-CQA	16.34±3.54 a	7.51±1.51 a	2.20±0.15 a

组别Group	CD4⁺/%	CD8⁺/%	CD4⁺/CD8⁺
H9N2	17.61±3.92 a	10.37±2.61 a	1.71±0.06 b
H9N2+4-CQA	19.20±3.73 a	8.75±2.03 a	2.21±0.08 a
对照组Control	19.74±4.77 a	9.52±2.16 a	2.07±0.03 a
4-CQA	16.34±3.54 a	7.51±1.51 a	2.20±0.15 a

Effect of cryptochlorogenic acid on immunomodulatory and antioxidant in mice of lung injury induced by H9N2-AIV

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