Effects of soaking seeds with 2, 4-epbrassinolide on starch netabolism during germination of barley seeds under drought stress

doi:10.3969/j.issn.1004-1524.2022.10.05

Acta Agriculturae Zhejiangensis ›› 2022, Vol. 34 ›› Issue (10): 2112-2120.DOI: 10.3969/j.issn.1004-1524.2022.10.05

• Crop Science • Previous Articles Next Articles

Effects of soaking seeds with 2, 4-epbrassinolide on starch netabolism during germination of barley seeds under drought stress

FENG Caijun(), SONG Ruijiao, SONG Lingyu, ZHANG Song, QI Juncang()

The Key Laboratory of Oasis Eco-Agriculture, Xinjiang Production and Construction Corps, College of Agriculture, Shihezi University, Shihezi 832003, Xinjiang, China

Received:2022-01-18 Online:2022-10-25 Published:2022-10-26
Contact: QI Juncang

Abstract

Abstract:

To study the effects of 2, 4-epbrassinolide on starch metabolism of barley seeds during germination under drought stress, XinPi No.6 barley seeds were soaked in brassinosteroid solution with different concentrations and cultured in 23% polyethylene glycol 6000 solution. The germination rate and seedling growth indexes were measured 72 h after germination, amylase activities and contents of starch, soluble total sugar, sucrose, fructose and glucose in barley seeds were determined at 12, 24, 36, 48, 60 and 72 h after germination. The results showed that drought stress significantly inhibited seed germination and amylase activities, increased starch contents and decreased soluble sugar contents. Compared with drought stress alone, application with suitable concentrations of brassinosteroid (5, 25 μg·L^-1) significantly increased germination rate, root length, bud length, coleoptile length, increased amylase activities and decreased starch contents in germinated seeds. The contents of soluble total sugar, glucose, sucrose and fructose were significantly increased under 25 μg·L^-1 brassinosteroid treatment, while higher concentration (200 μg·L^-1) inhibited the above indexes. These results suggested that suitable concentrations of brassinosteroid could enhance seed germination and seedling growth through promoting the rate of starch metabolism under drought stress.

Key words: barley (Hordeum vulgare L.), drought stress, brassinosteroid, germination, amylase, starch, soluble sugar

CLC Number:

S512.3

FENG Caijun, SONG Ruijiao, SONG Lingyu, ZHANG Song, QI Juncang. Effects of soaking seeds with 2, 4-epbrassinolide on starch netabolism during germination of barley seeds under drought stress[J]. Acta Agriculturae Zhejiangensis, 2022, 34(10): 2112-2120.

Figures/Tables 4

Table 1 Effects of brassinosteroid on seed germination and seedling growth of barley under drought stress

处理 Treatment	发芽率 Germination rate/%	胚芽长 Length of bud/cm	胚芽鞘长 Length of coleoptile/cm	胚根长 Length of root/cm	根数 Number of root
CK	99.33±1.15 a	5.69±0.17 a	3.06±0.25 a	6.85±0.28 a	5.80±0.20 a
D	30.67±6.11 d	1.22±0.07 c	1.45±0.11 de	1.95±0.14 de	5.67±0.23 a
E₅	44.00±5.29 c	1.41±0.10 b	1.87±0.31 bc	2.76±0.51 b	5.47±0.12 a
E₂₅	53.33±3.06 b	1.44±0.04 b	1.96±0.21 b	2.42±0.27 bc	5.53±0.12 a
E₅₀	32.67±5.03 d	1.24±0.02 c	1.61±0.17 cd	2.19±0.05 cd	5.67±0.31 a
E₁₀₀	26.67±5.03 d	1.15±0.06 c	1.35±0.10 de	1.82±0.06 de	5.60±0.20 a
E₂₀₀	14.67±3.06 e	1.15±0.07 c	1.17±0.04 e	1.68±0.05 e	5.57±0.38 a

Fig.1 Effects of brassinosteroid on activities of α amylase (A), β amylase (B) and total amylase (C) of barley seeds under drought stress Data was detected based on fresh weight. Data marked without the same lowercase letter in the same period indicated significant differences at P<0.05.The same as below.

Fig.2 Effects of brassinosteroid on contents of amylopectin (A), amylose (B) and total starch(C) in barley seeds under drought stress

Fig.3 Effects of brassinosteroid on the contents of soluble total sugar (A), glucose (B), sucrose (C) and fructose (D) in barley seeds under drought stress

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Effects of soaking seeds with 2, 4-epbrassinolide on starch netabolism during germination of barley seeds under drought stress

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